Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells
The surface-enhanced Raman scattering (SERS) bioprobe's strategy for identifying tumor cells always depended on the intensity difference of the Raman signal compared with that of normal cells. Hence, exploring novel SERS nanostructure with excellent spectra stability, a high enhancement factor...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/168932 |
| _version_ | 1811676654547763200 |
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| author | Lin, Jie Zhang, Dinghu Yu, Jian Pan, Ting Wu, Xiaoxia Chen, Tianxiang Gao, Changyong Chen, Chao Wang, Xiaotian Wu, Aiguo |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Lin, Jie Zhang, Dinghu Yu, Jian Pan, Ting Wu, Xiaoxia Chen, Tianxiang Gao, Changyong Chen, Chao Wang, Xiaotian Wu, Aiguo |
| author_sort | Lin, Jie |
| collection | NTU |
| description | The surface-enhanced Raman scattering (SERS) bioprobe's strategy for identifying tumor cells always depended on the intensity difference of the Raman signal compared with that of normal cells. Hence, exploring novel SERS nanostructure with excellent spectra stability, a high enhancement factor (EF), and good biocompatibility is a primary premise for boosting SERS signal reliability and accuracy of tumor cells. Here, high SERS EF (5.52 × 106) is acquired by developing novel amorphous nitrogen-doped carbon (NDC) nanocages (NCs), whose EF value was in a leading position among carbon-based SERS substrates. In addition, a uniform SERS signal was obtained on NDC NCs due to homogeneous morphology and size. The delocalized carbon-conjugated systems of graphitic-N, pyrrole-N, and pyridine-N with lone pair electrons increase the electronic density of states and reduce the electron localization function of NDC NCs, thereby promoting the charge transfer process. The electron-donor platform of the NDC NCs facilitates the thermodynamic process of charge transfer, resulting in multimode vibrational coupling in the surface complexes, which greatly amplifies the molecular polarizability. Importantly, the good biocompatibility and signal stability endow these NDC NC SERS bioprobes unique superiority in distinguishing tumor cells, and quantitative recognition of two triple-negative breast cancer cells based on SERS detection mode has been successfully realized. |
| first_indexed | 2024-10-01T02:24:55Z |
| format | Journal Article |
| id | ntu-10356/168932 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:24:55Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1689322023-06-23T01:37:02Z Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells Lin, Jie Zhang, Dinghu Yu, Jian Pan, Ting Wu, Xiaoxia Chen, Tianxiang Gao, Changyong Chen, Chao Wang, Xiaotian Wu, Aiguo School of Materials Science and Engineering Engineering::Materials Bioprobe Enhanced Raman Scattering The surface-enhanced Raman scattering (SERS) bioprobe's strategy for identifying tumor cells always depended on the intensity difference of the Raman signal compared with that of normal cells. Hence, exploring novel SERS nanostructure with excellent spectra stability, a high enhancement factor (EF), and good biocompatibility is a primary premise for boosting SERS signal reliability and accuracy of tumor cells. Here, high SERS EF (5.52 × 106) is acquired by developing novel amorphous nitrogen-doped carbon (NDC) nanocages (NCs), whose EF value was in a leading position among carbon-based SERS substrates. In addition, a uniform SERS signal was obtained on NDC NCs due to homogeneous morphology and size. The delocalized carbon-conjugated systems of graphitic-N, pyrrole-N, and pyridine-N with lone pair electrons increase the electronic density of states and reduce the electron localization function of NDC NCs, thereby promoting the charge transfer process. The electron-donor platform of the NDC NCs facilitates the thermodynamic process of charge transfer, resulting in multimode vibrational coupling in the surface complexes, which greatly amplifies the molecular polarizability. Importantly, the good biocompatibility and signal stability endow these NDC NC SERS bioprobes unique superiority in distinguishing tumor cells, and quantitative recognition of two triple-negative breast cancer cells based on SERS detection mode has been successfully realized. This work was supported by the funding from National Natural Science Foundation of China (52002380, 32025021, 31971292, 52022006, 12274018, 21875008 and 32111540257), Ningbo 3315 Innovative Teams Program (2019A-14-C), the Science and Technology Bureau of Ningbo City (2020Z094), Zhejiang Province Key Research Project (2019C03058, 2020C03110), Youth Innovation Promotion Association Foundation of CAS, and Zhejiang Provincial Natural Science Foundation of China (LQ20E020003). 2023-06-23T01:37:02Z 2023-06-23T01:37:02Z 2023 Journal Article Lin, J., Zhang, D., Yu, J., Pan, T., Wu, X., Chen, T., Gao, C., Chen, C., Wang, X. & Wu, A. (2023). Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells. Analytical Chemistry, 95(10), 4671-4681. https://dx.doi.org/10.1021/acs.analchem.2c05272 0003-2700 https://hdl.handle.net/10356/168932 10.1021/acs.analchem.2c05272 36735867 2-s2.0-85147442394 10 95 4671 4681 en Analytical Chemistry © 2023 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Materials Bioprobe Enhanced Raman Scattering Lin, Jie Zhang, Dinghu Yu, Jian Pan, Ting Wu, Xiaoxia Chen, Tianxiang Gao, Changyong Chen, Chao Wang, Xiaotian Wu, Aiguo Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title | Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title_full | Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title_fullStr | Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title_full_unstemmed | Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title_short | Amorphous nitrogen-doped carbon nanocages with excellent SERS sensitivity and stability for accurate identification of tumor cells |
| title_sort | amorphous nitrogen doped carbon nanocages with excellent sers sensitivity and stability for accurate identification of tumor cells |
| topic | Engineering::Materials Bioprobe Enhanced Raman Scattering |
| url | https://hdl.handle.net/10356/168932 |
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