Autonomous microlasers for profiling extracellular vesicles from cancer spheroids
Self-propelled micro/nanomotors are emergent intelligent sensors for analyzing extracellular biomarkers in circulating biological fluids. Conventional luminescent motors are often masked by a highly dynamic and scattered environment, creating challenges to characterize biomarkers or subtle binding d...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/168948 |
| _version_ | 1811691984725737472 |
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| author | Wang, Ziyihui Fang, Guocheng Gao, Zehang Liao, Yikai Gong, Chaoyang Kim, Munho Chang, Guo-En Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Wang, Ziyihui Fang, Guocheng Gao, Zehang Liao, Yikai Gong, Chaoyang Kim, Munho Chang, Guo-En Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng |
| author_sort | Wang, Ziyihui |
| collection | NTU |
| description | Self-propelled micro/nanomotors are emergent intelligent sensors for analyzing extracellular biomarkers in circulating biological fluids. Conventional luminescent motors are often masked by a highly dynamic and scattered environment, creating challenges to characterize biomarkers or subtle binding dynamics. Here we introduce a strategy to amplify subtle signals by coupling strong light-matter interactions on micromotors. A smart whispering-gallery-mode microlaser that can self-propel and analyze extracellular biomarkers is demonstrated through a liquid crystal microdroplet. Lasing spectral responses induced by cavity energy transfer were employed to reflect the abundance of protein biomarkers, generating exclusive molecular labels for cellular profiling of exosomes derived from 3D multicellular cancer spheroids. Finally, a microfluidic biosystem with different tumor-derived exosomes was employed to elaborate its sensing capability in complex environments. The proposed autonomous microlaser exhibits a promising method for both fundamental biological science and applications in drug screening, phenotyping, and organ-on-chip applications. |
| first_indexed | 2024-10-01T06:28:35Z |
| format | Journal Article |
| id | ntu-10356/168948 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:35Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1689482023-06-23T05:58:16Z Autonomous microlasers for profiling extracellular vesicles from cancer spheroids Wang, Ziyihui Fang, Guocheng Gao, Zehang Liao, Yikai Gong, Chaoyang Kim, Munho Chang, Guo-En Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Self-Propelled Motors Microlaser Self-propelled micro/nanomotors are emergent intelligent sensors for analyzing extracellular biomarkers in circulating biological fluids. Conventional luminescent motors are often masked by a highly dynamic and scattered environment, creating challenges to characterize biomarkers or subtle binding dynamics. Here we introduce a strategy to amplify subtle signals by coupling strong light-matter interactions on micromotors. A smart whispering-gallery-mode microlaser that can self-propel and analyze extracellular biomarkers is demonstrated through a liquid crystal microdroplet. Lasing spectral responses induced by cavity energy transfer were employed to reflect the abundance of protein biomarkers, generating exclusive molecular labels for cellular profiling of exosomes derived from 3D multicellular cancer spheroids. Finally, a microfluidic biosystem with different tumor-derived exosomes was employed to elaborate its sensing capability in complex environments. The proposed autonomous microlaser exhibits a promising method for both fundamental biological science and applications in drug screening, phenotyping, and organ-on-chip applications. Agency for Science, Technology and Research (A*STAR) This research is supported by A*STAR under its AME IRG Grant (Project No. A20E5c0085). Wang Z. would like to thank the support from the China Scholarship Council (Grant No. 202006250152). 2023-06-23T05:58:16Z 2023-06-23T05:58:16Z 2023 Journal Article Wang, Z., Fang, G., Gao, Z., Liao, Y., Gong, C., Kim, M., Chang, G., Feng, S., Xu, T., Liu, T. & Chen, Y. (2023). Autonomous microlasers for profiling extracellular vesicles from cancer spheroids. Nano Letters, 23(7), 2502-2510. https://dx.doi.org/10.1021/acs.nanolett.2c04123 1530-6984 https://hdl.handle.net/10356/168948 10.1021/acs.nanolett.2c04123 36926974 2-s2.0-85150451677 7 23 2502 2510 en A20E5c0085 Nano Letters © 2023 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Electrical and electronic engineering Self-Propelled Motors Microlaser Wang, Ziyihui Fang, Guocheng Gao, Zehang Liao, Yikai Gong, Chaoyang Kim, Munho Chang, Guo-En Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title | Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title_full | Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title_fullStr | Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title_full_unstemmed | Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title_short | Autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| title_sort | autonomous microlasers for profiling extracellular vesicles from cancer spheroids |
| topic | Engineering::Electrical and electronic engineering Self-Propelled Motors Microlaser |
| url | https://hdl.handle.net/10356/168948 |
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