Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169014 |
| _version_ | 1826116227133603840 |
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| author | Zhang, Liang Wang, Xiuping Chen, Yun Zhang, Baorui Xu, Hui Li, Chenchen Zhou, Yan |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Zhang, Liang Wang, Xiuping Chen, Yun Zhang, Baorui Xu, Hui Li, Chenchen Zhou, Yan |
| author_sort | Zhang, Liang |
| collection | NTU |
| description | In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic fermentation of mixed sludge without thermal hydrolysis process (THP) pretreatment. THP increased the MCFA production by approximately 128% in the anaerobic fermentation. During 102 days of operation, the fermentation of THP pre-treated mixed sludge stably generated 2.9 g COD/L MCFAs. The self-generated EDs could not maximize MCFA production, and external addition of ethanol improved MCFA yield. Caproiciproducens was the dominant chain-elongating bacteria. PICRUST2 revealed that both fatty acid biosynthesis and reverse β-oxidation pathways could participate in MCFA synthesis, and ethanol addition could enhance the contribution of the reverse β-oxidation pathway. Future studies should focus on the improvement of MCFA production from THP-assisted sludge fermentation. |
| first_indexed | 2024-10-01T04:07:52Z |
| format | Journal Article |
| id | ntu-10356/169014 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:07:52Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1690142023-06-27T02:29:26Z Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation Zhang, Liang Wang, Xiuping Chen, Yun Zhang, Baorui Xu, Hui Li, Chenchen Zhou, Yan School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Engineering::Environmental engineering Anaerobic Fermentation Thermal Hydrolysis Process In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic fermentation of mixed sludge without thermal hydrolysis process (THP) pretreatment. THP increased the MCFA production by approximately 128% in the anaerobic fermentation. During 102 days of operation, the fermentation of THP pre-treated mixed sludge stably generated 2.9 g COD/L MCFAs. The self-generated EDs could not maximize MCFA production, and external addition of ethanol improved MCFA yield. Caproiciproducens was the dominant chain-elongating bacteria. PICRUST2 revealed that both fatty acid biosynthesis and reverse β-oxidation pathways could participate in MCFA synthesis, and ethanol addition could enhance the contribution of the reverse β-oxidation pathway. Future studies should focus on the improvement of MCFA production from THP-assisted sludge fermentation. Nanyang Technological University The authors acknowledge the support from Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University. 2023-06-27T02:29:26Z 2023-06-27T02:29:26Z 2023 Journal Article Zhang, L., Wang, X., Chen, Y., Zhang, B., Xu, H., Li, C. & Zhou, Y. (2023). Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation. Bioresource Technology, 374, 128805-. https://dx.doi.org/10.1016/j.biortech.2023.128805 0960-8524 https://hdl.handle.net/10356/169014 10.1016/j.biortech.2023.128805 36849100 2-s2.0-85149277859 374 128805 en Bioresource Technology © 2023 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Environmental engineering Anaerobic Fermentation Thermal Hydrolysis Process Zhang, Liang Wang, Xiuping Chen, Yun Zhang, Baorui Xu, Hui Li, Chenchen Zhou, Yan Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title | Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title_full | Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title_fullStr | Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title_full_unstemmed | Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title_short | Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| title_sort | medium chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation |
| topic | Engineering::Environmental engineering Anaerobic Fermentation Thermal Hydrolysis Process |
| url | https://hdl.handle.net/10356/169014 |
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