Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes
The analysis of phyllosphere microbiomes traditionally relied on DNA extracted from whole leaves. To investigate the microbial communities on the adaxial (upper) and abaxial (lower) leaf surfaces, swabs were collected from both surfaces of two garden plants, Rhapis excelsa and Cordyline fruticosa. S...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169175 |
| _version_ | 1811677099557126144 |
|---|---|
| author | Lau, Kenny J. X. Gusareva, Elena S. Luhung, Irvan Premkrishnan, Balakrishnan N. V. Wong, Anthony Poh, Tuang Yeow Uchida, Akira Oliveira, Elaine L. Drautz-Moses, Daniela I. Junqueira, Ana Carolina M. Schuster, Stephan Christoph |
| author2 | Asian School of the Environment |
| author_facet | Asian School of the Environment Lau, Kenny J. X. Gusareva, Elena S. Luhung, Irvan Premkrishnan, Balakrishnan N. V. Wong, Anthony Poh, Tuang Yeow Uchida, Akira Oliveira, Elaine L. Drautz-Moses, Daniela I. Junqueira, Ana Carolina M. Schuster, Stephan Christoph |
| author_sort | Lau, Kenny J. X. |
| collection | NTU |
| description | The analysis of phyllosphere microbiomes traditionally relied on DNA extracted from whole leaves. To investigate the microbial communities on the adaxial (upper) and abaxial (lower) leaf surfaces, swabs were collected from both surfaces of two garden plants, Rhapis excelsa and Cordyline fruticosa. Samples were collected at noon and midnight and at five different locations to investigate if the phyllosphere microbial communities change with time and location. The abaxial surface of Rhapis excelsa and Cordyline fruticosa had fewer bacteria in contrast to its adaxial counterpart. This observation was consistent between noon and midnight and across five different locations. Our co-occurrence network analysis further showed that bacteria were found almost exclusively on the adaxial surface while only a small group of leaf blotch fungi thrived on the abaxial surface. There are higher densities of stomata on the abaxial surface and these openings are vulnerable ports of entry into the plant host. While one might argue about the settling of dust particles and microorganisms on the adaxial surface, we detected differences in reactive chemical activities and microstructures between the adaxial and abaxial surfaces. Our results further suggest that both plant species deploy different defence strategies to deter invading pathogens on the abaxial surface. We hypothesize that chemical and mechanical defence strategies evolved independently for harnessing and controlling phyllosphere microbiomes. Our findings have also advanced our understanding that the abaxial leaf surface is distinct from the adaxial surface and that the reduced microbial diversity is likely a consequence of plant-microbe interactions. |
| first_indexed | 2024-10-01T02:31:59Z |
| format | Journal Article |
| id | ntu-10356/169175 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:31:59Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1691752023-07-06T15:30:21Z Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes Lau, Kenny J. X. Gusareva, Elena S. Luhung, Irvan Premkrishnan, Balakrishnan N. V. Wong, Anthony Poh, Tuang Yeow Uchida, Akira Oliveira, Elaine L. Drautz-Moses, Daniela I. Junqueira, Ana Carolina M. Schuster, Stephan Christoph Asian School of the Environment Lee Kong Chian School of Medicine (LKCMedicine) Singapore Centre for Environmental Life Sciences and Engineering Translational Respiratory Research Laboratory Engineering::Bioengineering Microbial Diversity Microbiome The analysis of phyllosphere microbiomes traditionally relied on DNA extracted from whole leaves. To investigate the microbial communities on the adaxial (upper) and abaxial (lower) leaf surfaces, swabs were collected from both surfaces of two garden plants, Rhapis excelsa and Cordyline fruticosa. Samples were collected at noon and midnight and at five different locations to investigate if the phyllosphere microbial communities change with time and location. The abaxial surface of Rhapis excelsa and Cordyline fruticosa had fewer bacteria in contrast to its adaxial counterpart. This observation was consistent between noon and midnight and across five different locations. Our co-occurrence network analysis further showed that bacteria were found almost exclusively on the adaxial surface while only a small group of leaf blotch fungi thrived on the abaxial surface. There are higher densities of stomata on the abaxial surface and these openings are vulnerable ports of entry into the plant host. While one might argue about the settling of dust particles and microorganisms on the adaxial surface, we detected differences in reactive chemical activities and microstructures between the adaxial and abaxial surfaces. Our results further suggest that both plant species deploy different defence strategies to deter invading pathogens on the abaxial surface. We hypothesize that chemical and mechanical defence strategies evolved independently for harnessing and controlling phyllosphere microbiomes. Our findings have also advanced our understanding that the abaxial leaf surface is distinct from the adaxial surface and that the reduced microbial diversity is likely a consequence of plant-microbe interactions. Ministry of Education (MOE) Published version Our work was supported by an Academic Research Fund (AcRF) Tier 3 to SCS from the Singapore Ministry of Education (grant number: MOE2013-T3-1-013). 2023-07-05T00:18:08Z 2023-07-05T00:18:08Z 2023 Journal Article Lau, K. J. X., Gusareva, E. S., Luhung, I., Premkrishnan, B. N. V., Wong, A., Poh, T. Y., Uchida, A., Oliveira, E. L., Drautz-Moses, D. I., Junqueira, A. C. M. & Schuster, S. C. (2023). Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes. PloS One, 18(3), e0275734-. https://dx.doi.org/10.1371/journal.pone.0275734 1932-6203 https://hdl.handle.net/10356/169175 10.1371/journal.pone.0275734 36943839 2-s2.0-85150668644 3 18 e0275734 en MOE2013-T3-1-013 PloS One © 2023 Lau et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
| spellingShingle | Engineering::Bioengineering Microbial Diversity Microbiome Lau, Kenny J. X. Gusareva, Elena S. Luhung, Irvan Premkrishnan, Balakrishnan N. V. Wong, Anthony Poh, Tuang Yeow Uchida, Akira Oliveira, Elaine L. Drautz-Moses, Daniela I. Junqueira, Ana Carolina M. Schuster, Stephan Christoph Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title | Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title_full | Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title_fullStr | Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title_full_unstemmed | Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title_short | Structure vs. chemistry: alternate mechanisms for controlling leaf microbiomes |
| title_sort | structure vs chemistry alternate mechanisms for controlling leaf microbiomes |
| topic | Engineering::Bioengineering Microbial Diversity Microbiome |
| url | https://hdl.handle.net/10356/169175 |
| work_keys_str_mv | AT laukennyjx structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT gusarevaelenas structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT luhungirvan structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT premkrishnanbalakrishnannv structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT wonganthony structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT pohtuangyeow structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT uchidaakira structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT oliveiraelainel structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT drautzmosesdanielai structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT junqueiraanacarolinam structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes AT schusterstephanchristoph structurevschemistryalternatemechanismsforcontrollingleafmicrobiomes |