Single-shot isotropic differential interference contrast microscopy
Differential interference contrast (DIC) microscopy allows high-contrast, low-phototoxicity, and label-free imaging of transparent biological objects, and has been applied in the field of cellular morphology, cell segmentation, particle tracking, optical measurement and others. Commercial DIC micros...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169184 |
| _version_ | 1826124492026413056 |
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| author | Wang, Xinwei Wang, Hao Wang, Jinlu Liu, Xingsi Hao, Huijie Tan, You Sin Zhang, Yilei Zhang, He Ding, Xiangyan Zhao, Weisong Wang, Yuhang Lu, Zhengang Liu, Jian Yang, Joel K. W. Tan, Jiubin Li, Haoyu Qiu, Cheng-Wei Hu, Guangwei Ding, Xumin |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Wang, Xinwei Wang, Hao Wang, Jinlu Liu, Xingsi Hao, Huijie Tan, You Sin Zhang, Yilei Zhang, He Ding, Xiangyan Zhao, Weisong Wang, Yuhang Lu, Zhengang Liu, Jian Yang, Joel K. W. Tan, Jiubin Li, Haoyu Qiu, Cheng-Wei Hu, Guangwei Ding, Xumin |
| author_sort | Wang, Xinwei |
| collection | NTU |
| description | Differential interference contrast (DIC) microscopy allows high-contrast, low-phototoxicity, and label-free imaging of transparent biological objects, and has been applied in the field of cellular morphology, cell segmentation, particle tracking, optical measurement and others. Commercial DIC microscopy based on Nomarski or Wollaston prism resorts to the interference of two polarized waves with a lateral differential offset (shear) and axial phase shift (bias). However, the shear generated by these prisms is limited to the rectilinear direction, unfortunately resulting in anisotropic contrast imaging. Here we propose an ultracompact metasurface-assisted isotropic DIC (i-DIC) microscopy based on a grand original pattern of radial shear interferometry, that converts the rectilinear shear into rotationally symmetric along radial direction, enabling single-shot isotropic imaging capabilities. The i-DIC presents a complementary fusion of typical meta-optics, traditional microscopes and integrated optical system, and showcases the promising and synergetic advancements in edge detection, particle motion tracking, and label-free cellular imaging. |
| first_indexed | 2024-10-01T06:21:12Z |
| format | Journal Article |
| id | ntu-10356/169184 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:21:12Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1691842023-07-07T15:39:40Z Single-shot isotropic differential interference contrast microscopy Wang, Xinwei Wang, Hao Wang, Jinlu Liu, Xingsi Hao, Huijie Tan, You Sin Zhang, Yilei Zhang, He Ding, Xiangyan Zhao, Weisong Wang, Yuhang Lu, Zhengang Liu, Jian Yang, Joel K. W. Tan, Jiubin Li, Haoyu Qiu, Cheng-Wei Hu, Guangwei Ding, Xumin School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Fluorescence Imaging Scanning Electron Microscopy Differential interference contrast (DIC) microscopy allows high-contrast, low-phototoxicity, and label-free imaging of transparent biological objects, and has been applied in the field of cellular morphology, cell segmentation, particle tracking, optical measurement and others. Commercial DIC microscopy based on Nomarski or Wollaston prism resorts to the interference of two polarized waves with a lateral differential offset (shear) and axial phase shift (bias). However, the shear generated by these prisms is limited to the rectilinear direction, unfortunately resulting in anisotropic contrast imaging. Here we propose an ultracompact metasurface-assisted isotropic DIC (i-DIC) microscopy based on a grand original pattern of radial shear interferometry, that converts the rectilinear shear into rotationally symmetric along radial direction, enabling single-shot isotropic imaging capabilities. The i-DIC presents a complementary fusion of typical meta-optics, traditional microscopes and integrated optical system, and showcases the promising and synergetic advancements in edge detection, particle motion tracking, and label-free cellular imaging. Agency for Science, Technology and Research (A*STAR) National Research Foundation (NRF) Published version X.M.D. acknowledges funding from National Key R&D Program of China (2021YFF0603500), National Natural Science Foundation of China (62275063). H.W. and J.Y. acknowledge the support from NRF Investigatorship Award NRF-NRFI06-2020-0005. C.W.Q. acknowledges the support by AME Individual Research Grant (IRG) funded by A*STAR, Singapore (Grant No. A2083c0060). G.H. acknowledges the support from Optica Foundation and Startup Grant from Nanyang Technological University. 2023-07-05T01:43:04Z 2023-07-05T01:43:04Z 2023 Journal Article Wang, X., Wang, H., Wang, J., Liu, X., Hao, H., Tan, Y. S., Zhang, Y., Zhang, H., Ding, X., Zhao, W., Wang, Y., Lu, Z., Liu, J., Yang, J. K. W., Tan, J., Li, H., Qiu, C., Hu, G. & Ding, X. (2023). Single-shot isotropic differential interference contrast microscopy. Nature Communications, 14(1), 2063-. https://dx.doi.org/10.1038/s41467-023-37606-6 2041-1723 https://hdl.handle.net/10356/169184 10.1038/s41467-023-37606-6 37045869 2-s2.0-85152349295 1 14 2063 en Nature communications © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. application/pdf |
| spellingShingle | Engineering::Electrical and electronic engineering Fluorescence Imaging Scanning Electron Microscopy Wang, Xinwei Wang, Hao Wang, Jinlu Liu, Xingsi Hao, Huijie Tan, You Sin Zhang, Yilei Zhang, He Ding, Xiangyan Zhao, Weisong Wang, Yuhang Lu, Zhengang Liu, Jian Yang, Joel K. W. Tan, Jiubin Li, Haoyu Qiu, Cheng-Wei Hu, Guangwei Ding, Xumin Single-shot isotropic differential interference contrast microscopy |
| title | Single-shot isotropic differential interference contrast microscopy |
| title_full | Single-shot isotropic differential interference contrast microscopy |
| title_fullStr | Single-shot isotropic differential interference contrast microscopy |
| title_full_unstemmed | Single-shot isotropic differential interference contrast microscopy |
| title_short | Single-shot isotropic differential interference contrast microscopy |
| title_sort | single shot isotropic differential interference contrast microscopy |
| topic | Engineering::Electrical and electronic engineering Fluorescence Imaging Scanning Electron Microscopy |
| url | https://hdl.handle.net/10356/169184 |
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