The role of receptor uniformity in multivalent binding
Multivalency is prevalent in various biological systems and applications due to the superselectivity that arises from the cooperativity of multivalent binding. Traditionally, it was thought that weaker individual binding would improve the selectivity in multivalent targeting. Here, using analytical...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169307 |
| _version_ | 1826115483893497856 |
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| author | Xia, Xiuyang Zhang, Ge Ciamarra, Pica Massimo Jiao, Yang Ni, Ran |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Xia, Xiuyang Zhang, Ge Ciamarra, Pica Massimo Jiao, Yang Ni, Ran |
| author_sort | Xia, Xiuyang |
| collection | NTU |
| description | Multivalency is prevalent in various biological systems and applications due to the superselectivity that arises from the cooperativity of multivalent binding. Traditionally, it was thought that weaker individual binding would improve the selectivity in multivalent targeting. Here, using analytical mean field theory and Monte Carlo simulations, we discover that, for receptors that are highly uniformly distributed, the highest selectivity occurs at an intermediate binding energy and can be significantly greater than the weak binding limit. This is caused by an exponential relationship between the bound fraction and receptor concentration, which is influenced by both the strength and combinatorial entropy of binding. Our findings not only provide new guidelines for the rational design of biosensors using multivalent nanoparticles but also introduce a new perspective in understanding biological processes involving multivalency. |
| first_indexed | 2024-10-01T03:56:12Z |
| format | Journal Article |
| id | ntu-10356/169307 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:56:12Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1693072023-07-14T15:31:40Z The role of receptor uniformity in multivalent binding Xia, Xiuyang Zhang, Ge Ciamarra, Pica Massimo Jiao, Yang Ni, Ran School of Chemistry, Chemical Engineering and Biotechnology School of Physical and Mathematical Sciences Engineering::Chemical engineering Engineering::Bioengineering Multivalent Nanoparticle Binding Superselectivity Multivalency is prevalent in various biological systems and applications due to the superselectivity that arises from the cooperativity of multivalent binding. Traditionally, it was thought that weaker individual binding would improve the selectivity in multivalent targeting. Here, using analytical mean field theory and Monte Carlo simulations, we discover that, for receptors that are highly uniformly distributed, the highest selectivity occurs at an intermediate binding energy and can be significantly greater than the weak binding limit. This is caused by an exponential relationship between the bound fraction and receptor concentration, which is influenced by both the strength and combinatorial entropy of binding. Our findings not only provide new guidelines for the rational design of biosensors using multivalent nanoparticles but also introduce a new perspective in understanding biological processes involving multivalency. Ministry of Education (MOE) Published version This work is supported by the Academic Research Fund from Singapore Ministry of Education Tier 1 Gant (RG59/21) and Tier 2 Grant (MOE2019-T2-2-010). 2023-07-12T00:51:22Z 2023-07-12T00:51:22Z 2023 Journal Article Xia, X., Zhang, G., Ciamarra, P. M., Jiao, Y. & Ni, R. (2023). The role of receptor uniformity in multivalent binding. JACS Au, 3(5), 1385-1391. https://dx.doi.org/10.1021/jacsau.3c00052 2691-3704 https://hdl.handle.net/10356/169307 10.1021/jacsau.3c00052 37234107 2-s2.0-85154556771 5 3 1385 1391 en RG59/21 MOE2019-T2-2-010 JACS Au © 2023 The Authors. Published by American Chemical Society. This is an open access article distributed under the terms of the Creative Commons Attribution License. application/pdf |
| spellingShingle | Engineering::Chemical engineering Engineering::Bioengineering Multivalent Nanoparticle Binding Superselectivity Xia, Xiuyang Zhang, Ge Ciamarra, Pica Massimo Jiao, Yang Ni, Ran The role of receptor uniformity in multivalent binding |
| title | The role of receptor uniformity in multivalent binding |
| title_full | The role of receptor uniformity in multivalent binding |
| title_fullStr | The role of receptor uniformity in multivalent binding |
| title_full_unstemmed | The role of receptor uniformity in multivalent binding |
| title_short | The role of receptor uniformity in multivalent binding |
| title_sort | role of receptor uniformity in multivalent binding |
| topic | Engineering::Chemical engineering Engineering::Bioengineering Multivalent Nanoparticle Binding Superselectivity |
| url | https://hdl.handle.net/10356/169307 |
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