Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles
The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs)...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169424 |
| _version_ | 1811696239753822208 |
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| author | Shin, Gounhanul Hadinoto, Kunn Park, Jin-Won |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Shin, Gounhanul Hadinoto, Kunn Park, Jin-Won |
| author_sort | Shin, Gounhanul |
| collection | NTU |
| description | The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag2O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range. |
| first_indexed | 2024-10-01T07:36:12Z |
| format | Journal Article |
| id | ntu-10356/169424 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:36:12Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1694242023-12-29T06:52:28Z Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles Shin, Gounhanul Hadinoto, Kunn Park, Jin-Won School of Chemical and Biomedical Engineering Engineering::Chemical engineering Dopamine Monoamine Oxidase The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag2O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range. Published version This study was supported by the Research Program funded by the SeoulTech (Seoul National University of Science and Technology). 2023-07-18T05:08:26Z 2023-07-18T05:08:26Z 2023 Journal Article Shin, G., Hadinoto, K. & Park, J. (2023). Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles. Sensors and Materials, 35(5), 1767-1773. https://dx.doi.org/10.18494/SAM4356 0914-4935 https://hdl.handle.net/10356/169424 10.18494/SAM4356 2-s2.0-85162181068 5 35 1767 1773 en Sensors and Materials © MYU K.K. This work is licensed under a Creative Commons Attribution 4.0 International License. application/pdf |
| spellingShingle | Engineering::Chemical engineering Dopamine Monoamine Oxidase Shin, Gounhanul Hadinoto, Kunn Park, Jin-Won Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title | Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title_full | Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title_fullStr | Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title_full_unstemmed | Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title_short | Colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| title_sort | colorimetric dopamine detection based on immobilized monoamine oxidase through silver nanoparticles |
| topic | Engineering::Chemical engineering Dopamine Monoamine Oxidase |
| url | https://hdl.handle.net/10356/169424 |
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