Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galact...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/169795 |
| _version_ | 1826126338393636864 |
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| author | Teng, Rou Yi |
| author2 | Xiao Tianshu |
| author_facet | Xiao Tianshu Teng, Rou Yi |
| author_sort | Teng, Rou Yi |
| collection | NTU |
| description | With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galactosidase chemiluminescence. Although initial results were not the most favourable, adjustments in subsequent rounds have improved results. Experimental parameters will continue to be adjusted to further improve results, with hopes to move onto inhibitor studies. |
| first_indexed | 2024-10-01T06:51:05Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/169795 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:51:05Z |
| publishDate | 2023 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1697952023-09-12T08:22:38Z Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion Teng, Rou Yi Xiao Tianshu School of Biological Sciences tianshu.xiao@ntu.edu.sg Science::Biological sciences With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galactosidase chemiluminescence. Although initial results were not the most favourable, adjustments in subsequent rounds have improved results. Experimental parameters will continue to be adjusted to further improve results, with hopes to move onto inhibitor studies. Bachelor of Science in Biomedical Sciences 2023-08-04T02:18:58Z 2023-08-04T02:18:58Z 2023 Final Year Project (FYP) Teng, R. Y. (2023). Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/169795 https://hdl.handle.net/10356/169795 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Teng, Rou Yi Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title | Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title_full | Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title_fullStr | Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title_full_unstemmed | Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title_short | Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion |
| title_sort | developing a cell based assay to quantify and visualise dengue e protein mediated membrane fusion |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/169795 |
| work_keys_str_mv | AT tengrouyi developingacellbasedassaytoquantifyandvisualisedengueeproteinmediatedmembranefusion |