Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification
A long-standing issue for microfluidic impedance cytometry devices is the accuracy in determining the size of cells during counting and measurements. In this paper, we introduce a novel design that produces a homogeneous electric field in the sensing region and demonstrates higher accuracy than trad...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/169882 |
| _version_ | 1811683459597336576 |
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| author | Warren, Michael A. Shakouri, Amir Pacheco-Peña, Víctor Hallam, Toby |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Warren, Michael A. Shakouri, Amir Pacheco-Peña, Víctor Hallam, Toby |
| author_sort | Warren, Michael A. |
| collection | NTU |
| description | A long-standing issue for microfluidic impedance cytometry devices is the accuracy in determining the size of cells during counting and measurements. In this paper, we introduce a novel design that produces a homogeneous electric field in the sensing region and demonstrates higher accuracy than traditional designs in cell counting and sizing, reducing the reliance on cell focusing and signal postprocessing. The concept is validated, and the increased accuracy of the device over traditional designs is demonstrated through the use of finite element simulations to generate suitable data sets for particle trajectories and model expected signal variations. |
| first_indexed | 2024-10-01T04:13:04Z |
| format | Journal Article |
| id | ntu-10356/169882 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:13:04Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1698822023-08-14T15:34:37Z Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification Warren, Michael A. Shakouri, Amir Pacheco-Peña, Víctor Hallam, Toby School of Physical and Mathematical Sciences Science::Physics Flow Cytometry Optofluidics A long-standing issue for microfluidic impedance cytometry devices is the accuracy in determining the size of cells during counting and measurements. In this paper, we introduce a novel design that produces a homogeneous electric field in the sensing region and demonstrates higher accuracy than traditional designs in cell counting and sizing, reducing the reliance on cell focusing and signal postprocessing. The concept is validated, and the increased accuracy of the device over traditional designs is demonstrated through the use of finite element simulations to generate suitable data sets for particle trajectories and model expected signal variations. Published version 2023-08-11T03:32:48Z 2023-08-11T03:32:48Z 2023 Journal Article Warren, M. A., Shakouri, A., Pacheco-Peña, V. & Hallam, T. (2023). Development of a Novel Design of Microfluidic Impedance Cytometry for Improved Sensitivity and Cell Identification. ACS Omega, 8(21), 18882-18890. https://dx.doi.org/10.1021/acsomega.3c00797 2470-1343 https://hdl.handle.net/10356/169882 10.1021/acsomega.3c00797 37273599 2-s2.0-85160935183 21 8 18882 18890 en ACS Omega © 2023 The Authors. Published by American Chemical Society. This is an open-access article distributed under the terms of the Creative Commons Attribution License. application/pdf |
| spellingShingle | Science::Physics Flow Cytometry Optofluidics Warren, Michael A. Shakouri, Amir Pacheco-Peña, Víctor Hallam, Toby Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title | Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title_full | Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title_fullStr | Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title_full_unstemmed | Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title_short | Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| title_sort | development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification |
| topic | Science::Physics Flow Cytometry Optofluidics |
| url | https://hdl.handle.net/10356/169882 |
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