The FGFR inhibitor PD173074 binds to the C-terminus of oncofetal HMGA2 and modulates its DNA-binding and transcriptional activation functions

The FGFR inhibitor PD173074 binds to the C-terminus of oncofetal HMGA2 and modulates its DNA-binding and transcriptional activation functions

The architectural chromatin factor high-mobility group AT-hook 2 (HMGA2) is causally involved in several human malignancies and pathologies. HMGA2 is not expressed in most normal adult somatic cells, which renders the protein an attractive drug target. An established cell-based compound library scre...

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Bibliographic Details
Main Authors: Ahmed, Syed Moiz, Ragunathan, Priya, Shin, Joon, Peter, Sabrina, Kleissle, Sabrina, Neuenschwander, Martin, Schäfer, Reinhold, Kries, Jens Peter V., Grüber, Gerhard, Dröge, Peter
Other Authors: School of Biological Sciences
Format: Journal Article
Language:English
Published: 2023
Subjects:
Science::Biological sciences
AT-hook Domain
C-terminal Domain
Online Access:https://hdl.handle.net/10356/169924
Description
Summary:The architectural chromatin factor high-mobility group AT-hook 2 (HMGA2) is causally involved in several human malignancies and pathologies. HMGA2 is not expressed in most normal adult somatic cells, which renders the protein an attractive drug target. An established cell-based compound library screen identified the fibroblast growth factor receptor (FGFR) inhibitor PD173074 as an antagonist of HMGA2-mediated transcriptional reporter gene activation. We determined that PD173074 binds the C-terminus of HMGA2 and interferes with functional coordination of the three AT-hook DNA-binding domains mediated by the C-terminus. The HMGA2-antagonistic effect of PD173074 on transcriptional activation may therefore result from an induced altered DNA-binding mode of HMGA2. PD173074 as a novel HMGA2-specific antagonist could trigger the development of derivates with enhanced attributes and clinical potential.

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