Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate
Dynamic regulation of wound physiological signals is the basis of wound healing. Conventional biomaterials delivering growth factors to drive wound healing leads to the passive repair of soft tissues because of the mismatch of wound healing stages. Meanwhile, the bioactivity of wound exudate is ofte...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/170308 |
| _version_ | 1826118526419599360 |
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| author | Gao, Liheng Liu, Xingxing Zhao, Wenshuo Li, Chaojin Wang, Fujun Gao, Jing Liao, Xinqin Wei, Lei Wu, Hao Zheng, Yuanjin Wang, Lu |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Gao, Liheng Liu, Xingxing Zhao, Wenshuo Li, Chaojin Wang, Fujun Gao, Jing Liao, Xinqin Wei, Lei Wu, Hao Zheng, Yuanjin Wang, Lu |
| author_sort | Gao, Liheng |
| collection | NTU |
| description | Dynamic regulation of wound physiological signals is the basis of wound healing. Conventional biomaterials delivering growth factors to drive wound healing leads to the passive repair of soft tissues because of the mismatch of wound healing stages. Meanwhile, the bioactivity of wound exudate is often restricted by oxidation and bacterial contamination. Herein, an extracellular matrix mimicked nanofiber/hydrogel interpenetrated network (NFHIN) was constructed with a 3D nanofibrous framework for cell immigration, and interfiled aerogel containing cross-linked hyaluronic acid and hyperbranched polyamidoamine to balance the wound microenvironment. The aerogel can collect wound exudate and transform into a polycationic hydrogel with contact-killing effects even against intracellular pathogens (bactericidal rate > 99.9% in 30 min) and real-time scavenging property of reactive oxygen species. After co-culturing with the NFHIN, the bioactivity of fibroblast in theex vivoblister fluid was improved by 389.69%. The NFHIN showed sustainable exudate management with moisture-vapor transferring rate (6000 g m-2×24 h), equilibrium liquid content (75.3%), Young's modulus (115.1 ± 7 kPa), and anti-tearing behavior similar to human skin. The NFHIN can collect and activate wound exudate, turning it from a clinical problem to an autoimmune-derived wound regulation system, showing potential for wound care in critical skin diseases. |
| first_indexed | 2024-10-01T04:45:12Z |
| format | Journal Article |
| id | ntu-10356/170308 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:45:12Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1703082023-09-06T04:35:15Z Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate Gao, Liheng Liu, Xingxing Zhao, Wenshuo Li, Chaojin Wang, Fujun Gao, Jing Liao, Xinqin Wei, Lei Wu, Hao Zheng, Yuanjin Wang, Lu School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Polycationic Hyperbranched Polymer, Dynamic regulation of wound physiological signals is the basis of wound healing. Conventional biomaterials delivering growth factors to drive wound healing leads to the passive repair of soft tissues because of the mismatch of wound healing stages. Meanwhile, the bioactivity of wound exudate is often restricted by oxidation and bacterial contamination. Herein, an extracellular matrix mimicked nanofiber/hydrogel interpenetrated network (NFHIN) was constructed with a 3D nanofibrous framework for cell immigration, and interfiled aerogel containing cross-linked hyaluronic acid and hyperbranched polyamidoamine to balance the wound microenvironment. The aerogel can collect wound exudate and transform into a polycationic hydrogel with contact-killing effects even against intracellular pathogens (bactericidal rate > 99.9% in 30 min) and real-time scavenging property of reactive oxygen species. After co-culturing with the NFHIN, the bioactivity of fibroblast in theex vivoblister fluid was improved by 389.69%. The NFHIN showed sustainable exudate management with moisture-vapor transferring rate (6000 g m-2×24 h), equilibrium liquid content (75.3%), Young's modulus (115.1 ± 7 kPa), and anti-tearing behavior similar to human skin. The NFHIN can collect and activate wound exudate, turning it from a clinical problem to an autoimmune-derived wound regulation system, showing potential for wound care in critical skin diseases. The authors acknowledge the Natural Science Foundation of Shanghai (General Program, 22ZR1409500), Shanghai Science and Technology Innovation Action Plan (22S31905500), Medical Engineering Fund of Fudan University (yg2021-032), the Fundamental Research Funds for the Central Universities (No. 2232022G-01), the Fundamental Research Project of CNTAC (No. J202104), the Fundamental Research Funds for the Central Universities, and Graduate Student Innovation Fund of Donghua University (No. CUSF-DH-D-2021019), China Scholarship Council (No. 202006630061), 111 Project (No. BP0719035). 2023-09-06T04:35:15Z 2023-09-06T04:35:15Z 2023 Journal Article Gao, L., Liu, X., Zhao, W., Li, C., Wang, F., Gao, J., Liao, X., Wei, L., Wu, H., Zheng, Y. & Wang, L. (2023). Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate. Biofabrication, 15(1), 015021-. https://dx.doi.org/10.1088/1758-5090/acaa01 1758-5082 https://hdl.handle.net/10356/170308 10.1088/1758-5090/acaa01 36579621 2-s2.0-85145069215 1 15 015021 en Biofabrication © 2022 IOP Publishing Ltd. All rights reserved. |
| spellingShingle | Engineering::Electrical and electronic engineering Polycationic Hyperbranched Polymer, Gao, Liheng Liu, Xingxing Zhao, Wenshuo Li, Chaojin Wang, Fujun Gao, Jing Liao, Xinqin Wei, Lei Wu, Hao Zheng, Yuanjin Wang, Lu Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title | Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title_full | Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title_fullStr | Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title_full_unstemmed | Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title_short | Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate |
| title_sort | extracellular matrix mimicked 3d nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune derived healing regulation ability based on wound exudate |
| topic | Engineering::Electrical and electronic engineering Polycationic Hyperbranched Polymer, |
| url | https://hdl.handle.net/10356/170308 |
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