| סיכום: | Despite the exploration towards the environmentally friendly manufacturing process, two main challenges remain for lactic acid synthesis by microbial fermentation: resource cost and production efficiency. To alleviate environmental issues, lower raw material costs, and increase lactic acid manufacturing efficiency, this work aimed to evaluate characteristics of jackfruit seed starch (JFSS) extracted from fruit waste and explore the efficiency of lactic acid generation by co-fermentation of L. plantarum and B. subtilis in JFSS. JFSS consisted of a high amount of resistant starch and amylose, which was thermostable and displayed type A x-ray crystallization. Co-fermentation using JFSS as a substrate stimulated the growth of L. plantarum and enhanced lactic acid production over monocultures of L. plantarum and B. subtilis with insignificant by-product output. This study was valuable for improving lactic acid production efficiency and exploring the interaction between L. plantarum and B. subtilis.
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