A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples
Variations of malondialdehyde (MDA) level in biological samples often induce pathological changes, which is associated with various diseases. Here, we developed a combined surface-enhanced Raman spectroscopy (SERS) and colorimetric strategy for MDA quantitation. The methodology is based on the conde...
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/170564 |
| _version_ | 1826117953628667904 |
|---|---|
| author | Luo, Xiaojun Zhang, Shutong Xia, Zhichao Tan, Rui Li, Qiuju Qiao, Ling He, Yi Zhang, Guoqi Xu, Zhihong |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Luo, Xiaojun Zhang, Shutong Xia, Zhichao Tan, Rui Li, Qiuju Qiao, Ling He, Yi Zhang, Guoqi Xu, Zhihong |
| author_sort | Luo, Xiaojun |
| collection | NTU |
| description | Variations of malondialdehyde (MDA) level in biological samples often induce pathological changes, which is associated with various diseases. Here, we developed a combined surface-enhanced Raman spectroscopy (SERS) and colorimetric strategy for MDA quantitation. The methodology is based on the condensation reaction between 4-aminothiophenol (4-ATP)-modified Au nanoflowers (Au NFs) with the aldehyde groups of MDA, which causes the aggregation of the Au NFs and a concomitant change in the solution color from purple to blue and shifts in the local surface plasmon resonance band to longer wavelengths compared with monodisperse NFs. Additionally, after the condensation reaction, a new Raman peak ascribable to the CN vibration appeared at 1630 cm-1. The intensity of this peak was directly related to the concentration of MDA in solution, which allowed establishing the quantitative measurement of MDA based on SERS. The developed SERS assay displayed satisfactory sensitivity and selectivity with a broad linear range from 1.0 × 10-12 to 1.0 × 10-7 M and a low detection limit (∼3.6 × 10-13 M), outperforming other reported optical and electrochemical methods. Furthermore, the use of 4-ATP-modified Au NF probes to monitor MDA in human serum demonstrates the applicability of this combined SERS/colorimetric approach in a real environment. |
| first_indexed | 2024-10-01T04:35:28Z |
| format | Journal Article |
| id | ntu-10356/170564 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:35:28Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1705642023-09-19T06:11:51Z A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples Luo, Xiaojun Zhang, Shutong Xia, Zhichao Tan, Rui Li, Qiuju Qiao, Ling He, Yi Zhang, Guoqi Xu, Zhihong School of Physical and Mathematical Sciences Science::Chemistry Colorimetric Malondialdehyde Variations of malondialdehyde (MDA) level in biological samples often induce pathological changes, which is associated with various diseases. Here, we developed a combined surface-enhanced Raman spectroscopy (SERS) and colorimetric strategy for MDA quantitation. The methodology is based on the condensation reaction between 4-aminothiophenol (4-ATP)-modified Au nanoflowers (Au NFs) with the aldehyde groups of MDA, which causes the aggregation of the Au NFs and a concomitant change in the solution color from purple to blue and shifts in the local surface plasmon resonance band to longer wavelengths compared with monodisperse NFs. Additionally, after the condensation reaction, a new Raman peak ascribable to the CN vibration appeared at 1630 cm-1. The intensity of this peak was directly related to the concentration of MDA in solution, which allowed establishing the quantitative measurement of MDA based on SERS. The developed SERS assay displayed satisfactory sensitivity and selectivity with a broad linear range from 1.0 × 10-12 to 1.0 × 10-7 M and a low detection limit (∼3.6 × 10-13 M), outperforming other reported optical and electrochemical methods. Furthermore, the use of 4-ATP-modified Au NF probes to monitor MDA in human serum demonstrates the applicability of this combined SERS/colorimetric approach in a real environment. We greatly appreciate the financial support from PhD Scientific Research Startup Foundation of Xihua University ( RZ2100002826 ). L. Qiao thanks the China Scholarship Council (CSC) for its support (No. 202006860052). 2023-09-19T06:11:51Z 2023-09-19T06:11:51Z 2023 Journal Article Luo, X., Zhang, S., Xia, Z., Tan, R., Li, Q., Qiao, L., He, Y., Zhang, G. & Xu, Z. (2023). A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples. Analytica Chimica Acta, 1241, 340803-. https://dx.doi.org/10.1016/j.aca.2023.340803 0003-2670 https://hdl.handle.net/10356/170564 10.1016/j.aca.2023.340803 36657875 2-s2.0-85146537716 1241 340803 en Analytica Chimica Acta © 2023 Elsevier B.V. All rights reserved. |
| spellingShingle | Science::Chemistry Colorimetric Malondialdehyde Luo, Xiaojun Zhang, Shutong Xia, Zhichao Tan, Rui Li, Qiuju Qiao, Ling He, Yi Zhang, Guoqi Xu, Zhihong A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title | A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title_full | A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title_fullStr | A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title_full_unstemmed | A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title_short | A combined surface-enhanced Raman spectroscopy (SERS)/colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| title_sort | combined surface enhanced raman spectroscopy sers colorimetric approach for the sensitive detection of malondialdehyde in biological samples |
| topic | Science::Chemistry Colorimetric Malondialdehyde |
| url | https://hdl.handle.net/10356/170564 |
| work_keys_str_mv | AT luoxiaojun acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT zhangshutong acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT xiazhichao acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT tanrui acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT liqiuju acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT qiaoling acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT heyi acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT zhangguoqi acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT xuzhihong acombinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT luoxiaojun combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT zhangshutong combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT xiazhichao combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT tanrui combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT liqiuju combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT qiaoling combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT heyi combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT zhangguoqi combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples AT xuzhihong combinedsurfaceenhancedramanspectroscopyserscolorimetricapproachforthesensitivedetectionofmalondialdehydeinbiologicalsamples |