Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model
Methanococcus maripaludis can utilize CO2 as the sole carbon source. Since N2 is a major component of most flue gases, understanding its impact on the cellular behaviours of M. maripaludis is critical. Here, we studied CO2 conversion by M. maripaludis S2 in minimal media with ammonium or N2 as the s...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/170630 |
| _version_ | 1811678994945277952 |
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| author | Vo, Chi Hung Goyal, Nishu Kraft, Markus Karimi, Iftekhar A. |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Vo, Chi Hung Goyal, Nishu Kraft, Markus Karimi, Iftekhar A. |
| author_sort | Vo, Chi Hung |
| collection | NTU |
| description | Methanococcus maripaludis can utilize CO2 as the sole carbon source. Since N2 is a major component of most flue gases, understanding its impact on the cellular behaviours of M. maripaludis is critical. Here, we studied CO2 conversion by M. maripaludis S2 in minimal media with ammonium or N2 as the sole nitrogen source. N2-grown cultures exhibited a long lag phase, prolonged growth phase, and lower cell density compared to ammonium. At any specific growth rate, the CO2 intake and CH4 evolution fluxes were higher for N2 than ammonium, suggesting that N2 is a better nitrogen source for carbon conversion to CH4. The flux measurements were used to improve a genome-scale metabolic model existing in the literature. The revised model iMR557 has a coverage of 32% open reading frames and includes new pathways. iMR557 was used to illustrate flux distributions under different conditions and successfully replicate the previously reported acetate dissimilation. |
| first_indexed | 2024-10-01T03:02:07Z |
| format | Journal Article |
| id | ntu-10356/170630 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:02:07Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1706302023-09-25T01:27:51Z Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model Vo, Chi Hung Goyal, Nishu Kraft, Markus Karimi, Iftekhar A. School of Chemical and Biomedical Engineering Cambridge Centre for Advanced Research and Education in Singapore Ltd. Engineering::Chemical engineering Diazotrophy Methanogenesis Methanococcus maripaludis can utilize CO2 as the sole carbon source. Since N2 is a major component of most flue gases, understanding its impact on the cellular behaviours of M. maripaludis is critical. Here, we studied CO2 conversion by M. maripaludis S2 in minimal media with ammonium or N2 as the sole nitrogen source. N2-grown cultures exhibited a long lag phase, prolonged growth phase, and lower cell density compared to ammonium. At any specific growth rate, the CO2 intake and CH4 evolution fluxes were higher for N2 than ammonium, suggesting that N2 is a better nitrogen source for carbon conversion to CH4. The flux measurements were used to improve a genome-scale metabolic model existing in the literature. The revised model iMR557 has a coverage of 32% open reading frames and includes new pathways. iMR557 was used to illustrate flux distributions under different conditions and successfully replicate the previously reported acetate dissimilation. National Research Foundation (NRF) This research is supported by the National Research Foundation, Prime Minister’s Office, Singapore under its CREATE programme. Concomitantly, this work is supported by the National University of Singapore through its Graduate Research Scholarship. 2023-09-25T01:27:51Z 2023-09-25T01:27:51Z 2023 Journal Article Vo, C. H., Goyal, N., Kraft, M. & Karimi, I. A. (2023). Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model. Chemical Engineering Science, 278, 118910-. https://dx.doi.org/10.1016/j.ces.2023.118910 0009-2509 https://hdl.handle.net/10356/170630 10.1016/j.ces.2023.118910 2-s2.0-85160564174 278 118910 en Chemical Engineering Science © 2023 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Diazotrophy Methanogenesis Vo, Chi Hung Goyal, Nishu Kraft, Markus Karimi, Iftekhar A. Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title | Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title_full | Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title_fullStr | Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title_full_unstemmed | Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title_short | Carbon conversion by Methanococcus maripaludis S2 under diazotrophy and a revised genome-scale metabolic model |
| title_sort | carbon conversion by methanococcus maripaludis s2 under diazotrophy and a revised genome scale metabolic model |
| topic | Engineering::Chemical engineering Diazotrophy Methanogenesis |
| url | https://hdl.handle.net/10356/170630 |
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