Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques
For the past decade, tissue engineering has emerged rapidly as a promising alternative method to solve health problems, by means of repairing or replacing damaged tissues or organs. There are many progresses made on the methods and biomaterials used, to manufacture scaffolds with porous structure an...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/17075 |
| _version_ | 1811682173052256256 |
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| author | Ng, Zhen Yang. |
| author2 | Chua Chee Kai |
| author_facet | Chua Chee Kai Ng, Zhen Yang. |
| author_sort | Ng, Zhen Yang. |
| collection | NTU |
| description | For the past decade, tissue engineering has emerged rapidly as a promising alternative method to solve health problems, by means of repairing or replacing damaged tissues or organs. There are many progresses made on the methods and biomaterials used, to manufacture scaffolds with porous structure and nutrient rich environment that favour the growth of the tissue within the scaffolds. Due to the limitation of traditional fabrication techniques, which could not control the porous geometry, advanced manufacturing technologies, such as Rapid Prototyping techniques have been developed, to aid in overcoming the limitations.
As scaffold, which provide a nutrient rich environment is essential for cells to lay down new matrix and minimize cell necrosis; there is a need for continuous supply of nutrients throughout the scaffolds. This could be achieved by having interconnected channels within the scaffold to facilitate the delivery of the nutrients and waste in and out. Thus, scaffold with defined interconnected channels will aid in the processes of cell nutrient delivery, waste removal and vascular invasion.
Hence, for this project, gelatin scaffolds were fabricated via indirect rapid prototyping technique. Dissolvable molds that create porous scaffold with interconnected channel were fabricated using the rapid prototyping technique (Objet – Eden350V TM 3D inkjet printing process).
The results obtained showed that after dissolving the dissolvable mold, it was able to produce gelatin scaffold with defined interconnected channels after freeze-drying and cross-linking using the dehydrothermal oven. However, from the SEM images of the gelatin scaffold, they indicated weak interconnectivities between the pores inside the resulted scaffold. Thus, further work need to be done to improve the interconnectivity between the pores. Lastly, from the cytotoxicity assessment of the scaffold using the direct and indirect methods, it clearly showed that the scaffold was free from toxicity.
In conclusion, this project serves as a foundation for the research of tissue scaffold, specifically fabrication of gelatin scaffold using the indirect RP method of dissolvable mold. The project can be extended to a wider scope for practical application, and some suggestions on how the project can be extended were being discussed under future work. |
| first_indexed | 2024-10-01T03:52:37Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/17075 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:52:37Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/170752023-03-04T18:21:39Z Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques Ng, Zhen Yang. Chua Chee Kai Leong Kah Fai School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering::Prototyping DRNTU::Engineering::Bioengineering For the past decade, tissue engineering has emerged rapidly as a promising alternative method to solve health problems, by means of repairing or replacing damaged tissues or organs. There are many progresses made on the methods and biomaterials used, to manufacture scaffolds with porous structure and nutrient rich environment that favour the growth of the tissue within the scaffolds. Due to the limitation of traditional fabrication techniques, which could not control the porous geometry, advanced manufacturing technologies, such as Rapid Prototyping techniques have been developed, to aid in overcoming the limitations. As scaffold, which provide a nutrient rich environment is essential for cells to lay down new matrix and minimize cell necrosis; there is a need for continuous supply of nutrients throughout the scaffolds. This could be achieved by having interconnected channels within the scaffold to facilitate the delivery of the nutrients and waste in and out. Thus, scaffold with defined interconnected channels will aid in the processes of cell nutrient delivery, waste removal and vascular invasion. Hence, for this project, gelatin scaffolds were fabricated via indirect rapid prototyping technique. Dissolvable molds that create porous scaffold with interconnected channel were fabricated using the rapid prototyping technique (Objet – Eden350V TM 3D inkjet printing process). The results obtained showed that after dissolving the dissolvable mold, it was able to produce gelatin scaffold with defined interconnected channels after freeze-drying and cross-linking using the dehydrothermal oven. However, from the SEM images of the gelatin scaffold, they indicated weak interconnectivities between the pores inside the resulted scaffold. Thus, further work need to be done to improve the interconnectivity between the pores. Lastly, from the cytotoxicity assessment of the scaffold using the direct and indirect methods, it clearly showed that the scaffold was free from toxicity. In conclusion, this project serves as a foundation for the research of tissue scaffold, specifically fabrication of gelatin scaffold using the indirect RP method of dissolvable mold. The project can be extended to a wider scope for practical application, and some suggestions on how the project can be extended were being discussed under future work. Bachelor of Engineering 2009-05-29T06:26:37Z 2009-05-29T06:26:37Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/17075 en Nanyang Technological University 73 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering::Prototyping DRNTU::Engineering::Bioengineering Ng, Zhen Yang. Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title | Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title_full | Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title_fullStr | Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title_full_unstemmed | Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title_short | Fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| title_sort | fabrication of naturally derived scaffolds using indirect rapid prototyping techniques |
| topic | DRNTU::Engineering::Mechanical engineering::Prototyping DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/17075 |
| work_keys_str_mv | AT ngzhenyang fabricationofnaturallyderivedscaffoldsusingindirectrapidprototypingtechniques |