High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner
There is a close relationship between the SARS-CoV-2 virus and lipoproteins, in particular high-density lipoprotein (HDL). The severity of the coronavirus disease 2019 (COVID-19) is inversely correlated with HDL plasma levels. It is known that the SARS-CoV-2 spike (S) protein binds the HDL particle,...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Online Access: | https://hdl.handle.net/10356/170890 |
| _version_ | 1824456515900145664 |
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| author | Correa, Yubexi Del Giudice, Rita Waldie, Sarah Thépaut, Michel Micciula, Samantha Gerelli, Yuri Moulin, Martine Delaunay, Clara Fieschi, Franck Pichler, Harald Haertlein, Michael Forsyth, V. Trevor Le Brun, Anton Moir, Michael Russell, Robert A. Darwish, Tamim Brinck, Jonas Wodaje, Tigist Jansen, Martin Martín, César Roosen-Runge, Felix Cárdenas, Marité |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Correa, Yubexi Del Giudice, Rita Waldie, Sarah Thépaut, Michel Micciula, Samantha Gerelli, Yuri Moulin, Martine Delaunay, Clara Fieschi, Franck Pichler, Harald Haertlein, Michael Forsyth, V. Trevor Le Brun, Anton Moir, Michael Russell, Robert A. Darwish, Tamim Brinck, Jonas Wodaje, Tigist Jansen, Martin Martín, César Roosen-Runge, Felix Cárdenas, Marité |
| author_sort | Correa, Yubexi |
| collection | NTU |
| description | There is a close relationship between the SARS-CoV-2 virus and lipoproteins, in particular high-density lipoprotein (HDL). The severity of the coronavirus disease 2019 (COVID-19) is inversely correlated with HDL plasma levels. It is known that the SARS-CoV-2 spike (S) protein binds the HDL particle, probably depleting it of lipids and altering HDL function. Based on neutron reflectometry (NR) and the ability of HDL to efflux cholesterol from macrophages, we confirm these observations and further identify the preference of the S protein for specific lipids and the consequent effects on HDL function on lipid exchange ability. Moreover, the effect of the S protein on HDL function differs depending on the individuals lipid serum profile. Contrasting trends were observed for individuals presenting low triglycerides/high cholesterol serum levels (LTHC) compared to high triglycerides/high cholesterol (HTHC) or low triglycerides/low cholesterol serum levels (LTLC). Collectively, these results suggest that the S protein interacts with the HDL particle and, depending on the lipid profile of the infected individual, it impairs its function during COVID-19 infection, causing an imbalance in lipid metabolism. |
| first_indexed | 2025-02-19T03:55:20Z |
| format | Journal Article |
| id | ntu-10356/170890 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:55:20Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1708902023-10-09T15:32:20Z High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner Correa, Yubexi Del Giudice, Rita Waldie, Sarah Thépaut, Michel Micciula, Samantha Gerelli, Yuri Moulin, Martine Delaunay, Clara Fieschi, Franck Pichler, Harald Haertlein, Michael Forsyth, V. Trevor Le Brun, Anton Moir, Michael Russell, Robert A. Darwish, Tamim Brinck, Jonas Wodaje, Tigist Jansen, Martin Martín, César Roosen-Runge, Felix Cárdenas, Marité School of Biological Sciences Science::Biological sciences COVID-19 Deuterated Cholesterol There is a close relationship between the SARS-CoV-2 virus and lipoproteins, in particular high-density lipoprotein (HDL). The severity of the coronavirus disease 2019 (COVID-19) is inversely correlated with HDL plasma levels. It is known that the SARS-CoV-2 spike (S) protein binds the HDL particle, probably depleting it of lipids and altering HDL function. Based on neutron reflectometry (NR) and the ability of HDL to efflux cholesterol from macrophages, we confirm these observations and further identify the preference of the S protein for specific lipids and the consequent effects on HDL function on lipid exchange ability. Moreover, the effect of the S protein on HDL function differs depending on the individuals lipid serum profile. Contrasting trends were observed for individuals presenting low triglycerides/high cholesterol serum levels (LTHC) compared to high triglycerides/high cholesterol (HTHC) or low triglycerides/low cholesterol serum levels (LTLC). Collectively, these results suggest that the S protein interacts with the HDL particle and, depending on the lipid profile of the infected individual, it impairs its function during COVID-19 infection, causing an imbalance in lipid metabolism. Published version MC, YC, and SW acknowledge economic support from the Swedish Research Council (grants number 2018-03990 and 2018-04833). MC thanks the Wennergren Foundation for funding (grant number SSh2020- 0005). IBS acknowledges integration into the Interdisciplinary Research Institute of Grenoble (IRIG, CEA). VTF acknowledges support from the UK Engineering and Physical Sciences Council (EPSRC) which funded the creation of the Deuteration Laboratory within the Life Sciences Group at the ILL [GR/R99393/01 and EP/C015452/1]. This work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). Operations for the Spatz neutron reflectometer and the National Deuteration Facility at ANSTO are supported by the National Characterisation Research Infrastructure Strategy (NCRIS), an Australian Government initiative. 2023-10-04T08:12:14Z 2023-10-04T08:12:14Z 2023 Journal Article Correa, Y., Del Giudice, R., Waldie, S., Thépaut, M., Micciula, S., Gerelli, Y., Moulin, M., Delaunay, C., Fieschi, F., Pichler, H., Haertlein, M., Forsyth, V. T., Le Brun, A., Moir, M., Russell, R. A., Darwish, T., Brinck, J., Wodaje, T., Jansen, M., ...Cárdenas, M. (2023). High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner. Journal of Colloid and Interface Science, 645, 627-638. https://dx.doi.org/10.1016/j.jcis.2023.04.137 0021-9797 https://hdl.handle.net/10356/170890 10.1016/j.jcis.2023.04.137 37167912 2-s2.0-85158888783 645 627 638 en Journal of Colloid and Interface Science © 2023 Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Science::Biological sciences COVID-19 Deuterated Cholesterol Correa, Yubexi Del Giudice, Rita Waldie, Sarah Thépaut, Michel Micciula, Samantha Gerelli, Yuri Moulin, Martine Delaunay, Clara Fieschi, Franck Pichler, Harald Haertlein, Michael Forsyth, V. Trevor Le Brun, Anton Moir, Michael Russell, Robert A. Darwish, Tamim Brinck, Jonas Wodaje, Tigist Jansen, Martin Martín, César Roosen-Runge, Felix Cárdenas, Marité High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title | High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title_full | High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title_fullStr | High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title_full_unstemmed | High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title_short | High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner |
| title_sort | high density lipoprotein function is modulated by the sars cov 2 spike protein in a lipid type dependent manner |
| topic | Science::Biological sciences COVID-19 Deuterated Cholesterol |
| url | https://hdl.handle.net/10356/170890 |
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