Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer)
The Asian seabass is of importance both as a farmed and wild animal. With the emergence of infectious diseases, there is a need to understand and characterize the immune system. In humans, the highly polymorphic MHC class I (MHC-I) molecules play an important role in antigen presentation for the ada...
| Principais autores: | , , , , , |
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| Formato: | Journal Article |
| Idioma: | English |
| Publicado em: |
2023
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| Assuntos: | |
| Acesso em linha: | https://hdl.handle.net/10356/170997 |
| _version_ | 1826116363960188928 |
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| author | Loh, Zhixuan Huan, Xuelu Awate, Sunita Schrittwieser, Markus Renia, Laurent Ren, Ee Chee |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Loh, Zhixuan Huan, Xuelu Awate, Sunita Schrittwieser, Markus Renia, Laurent Ren, Ee Chee |
| author_sort | Loh, Zhixuan |
| collection | NTU |
| description | The Asian seabass is of importance both as a farmed and wild animal. With the emergence of infectious diseases, there is a need to understand and characterize the immune system. In humans, the highly polymorphic MHC class I (MHC-I) molecules play an important role in antigen presentation for the adaptive immune system. In the present study, we characterized a single MHC-I gene in Asian seabass (Lates calcarifer) by amplifying and sequencing the MHC-I alpha 1 and alpha 2 domains, followed by multi-sequence alignment analyses. The results indicated that the Asian seabass MHC-I α1 and α2 domain sequences showed an overall similarity within Asian seabass and retained the majority of the conserved binding residues of human leukocyte antigen-A2 (HLA-A2). Phylogenetic tree analysis revealed that the sequences belonged to the U lineage. Mapping the conserved binding residue positions on human HLA-A2 and grass carp crystal structure showed a high degree of similarity. In conclusion, the availability of MHC-I α1 and α2 sequences enhances the quality of MHC class I genetic information in Asian seabass, providing new tools to analyze fish immune responses to pathogen infections, and will be applicable in the study of the phylogeny and the evolution of antigen-specific receptors. |
| first_indexed | 2024-10-01T04:10:10Z |
| format | Journal Article |
| id | ntu-10356/170997 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:10:10Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1709972023-10-15T15:38:07Z Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) Loh, Zhixuan Huan, Xuelu Awate, Sunita Schrittwieser, Markus Renia, Laurent Ren, Ee Chee Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences Science::Biological sciences Asian Seabass Lates Calcarifer The Asian seabass is of importance both as a farmed and wild animal. With the emergence of infectious diseases, there is a need to understand and characterize the immune system. In humans, the highly polymorphic MHC class I (MHC-I) molecules play an important role in antigen presentation for the adaptive immune system. In the present study, we characterized a single MHC-I gene in Asian seabass (Lates calcarifer) by amplifying and sequencing the MHC-I alpha 1 and alpha 2 domains, followed by multi-sequence alignment analyses. The results indicated that the Asian seabass MHC-I α1 and α2 domain sequences showed an overall similarity within Asian seabass and retained the majority of the conserved binding residues of human leukocyte antigen-A2 (HLA-A2). Phylogenetic tree analysis revealed that the sequences belonged to the U lineage. Mapping the conserved binding residue positions on human HLA-A2 and grass carp crystal structure showed a high degree of similarity. In conclusion, the availability of MHC-I α1 and α2 sequences enhances the quality of MHC class I genetic information in Asian seabass, providing new tools to analyze fish immune responses to pathogen infections, and will be applicable in the study of the phylogeny and the evolution of antigen-specific receptors. Singapore Food Agency Published version This research is supported by the Singapore Food Agency under the Singapore Food Story ("SFS") R&D Programme 1st Grant Call (Theme 1 "Sustainable Urban Food Production") (SFS_RND_SUFP_001_01). 2023-10-10T02:56:23Z 2023-10-10T02:56:23Z 2022 Journal Article Loh, Z., Huan, X., Awate, S., Schrittwieser, M., Renia, L. & Ren, E. C. (2022). Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer). International Journal of Molecular Sciences, 23(18), 10688-. https://dx.doi.org/10.3390/ijms231810688 1661-6596 https://hdl.handle.net/10356/170997 10.3390/ijms231810688 36142628 2-s2.0-85138407886 18 23 10688 en SFS_RND_SUFP_001_01 International Journal of Molecular Sciences © 2022 The authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Asian Seabass Lates Calcarifer Loh, Zhixuan Huan, Xuelu Awate, Sunita Schrittwieser, Markus Renia, Laurent Ren, Ee Chee Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title | Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title_full | Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title_fullStr | Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title_full_unstemmed | Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title_short | Molecular characterization of MHC class I alpha 1 and 2 domains in Asian seabass (lates calcarifer) |
| title_sort | molecular characterization of mhc class i alpha 1 and 2 domains in asian seabass lates calcarifer |
| topic | Science::Biological sciences Asian Seabass Lates Calcarifer |
| url | https://hdl.handle.net/10356/170997 |
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