Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach
Childhood obesity carries an increased risk of metabolic complications, sleep disturbances, and cancer. Visceral adiposity is independently associated with inflammation and insulin resistance in obese children. However, the underlying pathogenic mechanisms are still unclear. We aimed to detect the g...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Online Access: | https://hdl.handle.net/10356/171013 |
| _version_ | 1811678893935951872 |
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| author | Roy, Dipayan Modi, Anupama Ghosh, Ritwik Ghosh, Raghumoy Benito-León, Julian |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Roy, Dipayan Modi, Anupama Ghosh, Ritwik Ghosh, Raghumoy Benito-León, Julian |
| author_sort | Roy, Dipayan |
| collection | NTU |
| description | Childhood obesity carries an increased risk of metabolic complications, sleep disturbances, and cancer. Visceral adiposity is independently associated with inflammation and insulin resistance in obese children. However, the underlying pathogenic mechanisms are still unclear. We aimed to detect the gene expression pattern and its regulatory network in the visceral adipose tissue of obese pediatric individuals. Using differentially-expressed genes (DEGs) identified from two publicly available datasets, GSE9624 and GSE88837, we performed functional enrichment, protein–protein interaction, and network analyses to identify pathways, targeting transcription factors (TFs), microRNA (miRNA), and regulatory networks. There were 184 overlapping DEGs with six significant clusters and 19 candidate hub genes. Furthermore, 24 TFs targeted these hub genes. The genes were regulated by miR-16-5p, miR-124-3p, miR-103a-3p, and miR-107, the top miRNA, according to a maximum number of miRNA–mRNA interaction pairs. The miRNA were significantly enriched in several pathways, including lipid metabolism, immune response, vascular inflammation, and brain development, and were associated with prediabetes, diabetic nephropathy, depression, solid tumors, and multiple sclerosis. The genes and miRNA detected in this study involve pathways and diseases related to obesity and obesity-associated complications. The results emphasize the importance of the TGF-β signaling pathway and its regulatory molecules, the immune system, and the adipocytic apoptotic pathway in pediatric obesity. The networks associated with this condition and the molecular mechanisms through which the potential regulators contribute to pathogenesis are open to investigation. |
| first_indexed | 2024-10-01T03:00:30Z |
| format | Journal Article |
| id | ntu-10356/171013 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:00:30Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1710132023-10-15T15:37:56Z Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach Roy, Dipayan Modi, Anupama Ghosh, Ritwik Ghosh, Raghumoy Benito-León, Julian Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Childhood Obesity in Silico Childhood obesity carries an increased risk of metabolic complications, sleep disturbances, and cancer. Visceral adiposity is independently associated with inflammation and insulin resistance in obese children. However, the underlying pathogenic mechanisms are still unclear. We aimed to detect the gene expression pattern and its regulatory network in the visceral adipose tissue of obese pediatric individuals. Using differentially-expressed genes (DEGs) identified from two publicly available datasets, GSE9624 and GSE88837, we performed functional enrichment, protein–protein interaction, and network analyses to identify pathways, targeting transcription factors (TFs), microRNA (miRNA), and regulatory networks. There were 184 overlapping DEGs with six significant clusters and 19 candidate hub genes. Furthermore, 24 TFs targeted these hub genes. The genes were regulated by miR-16-5p, miR-124-3p, miR-103a-3p, and miR-107, the top miRNA, according to a maximum number of miRNA–mRNA interaction pairs. The miRNA were significantly enriched in several pathways, including lipid metabolism, immune response, vascular inflammation, and brain development, and were associated with prediabetes, diabetic nephropathy, depression, solid tumors, and multiple sclerosis. The genes and miRNA detected in this study involve pathways and diseases related to obesity and obesity-associated complications. The results emphasize the importance of the TGF-β signaling pathway and its regulatory molecules, the immune system, and the adipocytic apoptotic pathway in pediatric obesity. The networks associated with this condition and the molecular mechanisms through which the potential regulators contribute to pathogenesis are open to investigation. Published version 2023-10-10T05:10:50Z 2023-10-10T05:10:50Z 2022 Journal Article Roy, D., Modi, A., Ghosh, R., Ghosh, R. & Benito-León, J. (2022). Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach. International Journal of Molecular Sciences, 23(19), 11036-. https://dx.doi.org/10.3390/ijms231911036 1661-6596 https://hdl.handle.net/10356/171013 10.3390/ijms231911036 23 2-s2.0-85139939451 19 23 11036 en International Journal of Molecular Sciences © 2022 The authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Medicine Childhood Obesity in Silico Roy, Dipayan Modi, Anupama Ghosh, Ritwik Ghosh, Raghumoy Benito-León, Julian Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title | Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title_full | Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title_fullStr | Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title_full_unstemmed | Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title_short | Visceral adipose tissue molecular networks and regulatory microRNA in pediatric obesity: an in silico approach |
| title_sort | visceral adipose tissue molecular networks and regulatory microrna in pediatric obesity an in silico approach |
| topic | Science::Medicine Childhood Obesity in Silico |
| url | https://hdl.handle.net/10356/171013 |
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