Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity
Recent studies indicate that carbon monoxide-releasing molecules (CORMs), a class of organometallic compounds, exert antibacterial activities through the delivery of carbon monoxide (CO) molecules. We developed a new-class CO-delivery system by conjugating classical low-molecular-weight CORMs (i.e.,...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2023
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| Online Access: | https://hdl.handle.net/10356/171324 |
| _version_ | 1811697700115054592 |
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| author | Wang, Liping Zhong, Wenbin Liu, Bo Pranantyo, Dicky Chan-Park, Mary B. |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Wang, Liping Zhong, Wenbin Liu, Bo Pranantyo, Dicky Chan-Park, Mary B. |
| author_sort | Wang, Liping |
| collection | NTU |
| description | Recent studies indicate that carbon monoxide-releasing molecules (CORMs), a class of organometallic compounds, exert antibacterial activities through the delivery of carbon monoxide (CO) molecules. We developed a new-class CO-delivery system by conjugating classical low-molecular-weight CORMs (i.e., [Ru(CO)3Cl2]2 and Mn(CO)5Br) onto a positively charged carrier, polyimidazolium (PIM), giving cationic CO-releasing polymers Ru@PIM and Mn@PIM, respectively. Compared with low-molecular-weight CORMs, our polymeric CO vehicles showed improved water solubility, reduced cytotoxicity, significantly extended CO-releasing duration, and enhanced antimicrobial ability against both planktonic and biofilm microorganisms. Ru@PIM and Mn@PIM inhibited the growth of a broad spectrum of free Gram-positive and Gram-negative bacteria as well as fungus with the lowest minimum inhibitory concentration (MIC) at 8 μg/mL. They were effective in preventing pathogenic Pseudomonas aeruginosa biofilm formation with biofilm reduction by more than 92% at 16 μg/mL and 99% at 32 μg/mL. They also demonstrated potent dispersal efficacy on recalcitrant well-established biofilms through a synergetic activity with a biofilm log10 reduction of 2.5-3.2 ≥ 64 μg/mL and nearly 2.0 at the concentration of as low as 16 μg/mL. This CO-releasing system may retain long-time antimicrobial ability after the complete release of CO molecules owing to the cationic structure. The novel CO-releasing polymers have great potential as antimicrobial and antibiofilm agents in biomedical applications. |
| first_indexed | 2024-10-01T07:59:25Z |
| format | Journal Article |
| id | ntu-10356/171324 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:59:25Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1713242023-10-19T02:32:39Z Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity Wang, Liping Zhong, Wenbin Liu, Bo Pranantyo, Dicky Chan-Park, Mary B. School of Chemistry, Chemical Engineering and Biotechnology Centre for Antimicrobial Bioengineering Engineering::Bioengineering Antimicrobial Antibiofilm Recent studies indicate that carbon monoxide-releasing molecules (CORMs), a class of organometallic compounds, exert antibacterial activities through the delivery of carbon monoxide (CO) molecules. We developed a new-class CO-delivery system by conjugating classical low-molecular-weight CORMs (i.e., [Ru(CO)3Cl2]2 and Mn(CO)5Br) onto a positively charged carrier, polyimidazolium (PIM), giving cationic CO-releasing polymers Ru@PIM and Mn@PIM, respectively. Compared with low-molecular-weight CORMs, our polymeric CO vehicles showed improved water solubility, reduced cytotoxicity, significantly extended CO-releasing duration, and enhanced antimicrobial ability against both planktonic and biofilm microorganisms. Ru@PIM and Mn@PIM inhibited the growth of a broad spectrum of free Gram-positive and Gram-negative bacteria as well as fungus with the lowest minimum inhibitory concentration (MIC) at 8 μg/mL. They were effective in preventing pathogenic Pseudomonas aeruginosa biofilm formation with biofilm reduction by more than 92% at 16 μg/mL and 99% at 32 μg/mL. They also demonstrated potent dispersal efficacy on recalcitrant well-established biofilms through a synergetic activity with a biofilm log10 reduction of 2.5-3.2 ≥ 64 μg/mL and nearly 2.0 at the concentration of as low as 16 μg/mL. This CO-releasing system may retain long-time antimicrobial ability after the complete release of CO molecules owing to the cationic structure. The novel CO-releasing polymers have great potential as antimicrobial and antibiofilm agents in biomedical applications. Ministry of Education (MOE) Nanyang Technological University The authors thank the funding support from Singapore Ministry of Education Tier 3 grants (MOE2013-T3-1-002, MOE2018-T3-1-003) and NTU. L.P.W. and W.B.Z. acknowledge the support of NTU Ph.D. scholarships. 2023-10-19T02:32:38Z 2023-10-19T02:32:38Z 2023 Journal Article Wang, L., Zhong, W., Liu, B., Pranantyo, D. & Chan-Park, M. B. (2023). Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity. ACS Applied Materials and Interfaces, 15(35), 41772-41782. https://dx.doi.org/10.1021/acsami.3c02898 1944-8244 https://hdl.handle.net/10356/171324 10.1021/acsami.3c02898 37609827 2-s2.0-85169846862 35 15 41772 41782 en MOE2013-T3-1-002 MOE2018-T3-1-003 ACS Applied Materials and Interfaces © 2023 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Antimicrobial Antibiofilm Wang, Liping Zhong, Wenbin Liu, Bo Pranantyo, Dicky Chan-Park, Mary B. Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title | Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title_full | Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title_fullStr | Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title_full_unstemmed | Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title_short | Cationic carbon monoxide-releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| title_sort | cationic carbon monoxide releasing polymers as antimicrobial and antibiofilm agents by the synergetic activity |
| topic | Engineering::Bioengineering Antimicrobial Antibiofilm |
| url | https://hdl.handle.net/10356/171324 |
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