Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor
A liquid crystal (LC)-based optofluidic whispering gallery mode (WGM) resonator has been applied as a biosensor to detect biotin. Immobilized streptavidin (SA) act as protein molecules and specifically bind to biotin through strong non-covalent interaction, which can interfere with the orientation o...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/171551 |
| _version_ | 1826114865442324480 |
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| author | Wang, Haonan Xu, Tianhua Wang, Ziyihui Liu, Yize Chen, Huaixu Jiang, Junfeng Liu, Tiegen |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Wang, Haonan Xu, Tianhua Wang, Ziyihui Liu, Yize Chen, Huaixu Jiang, Junfeng Liu, Tiegen |
| author_sort | Wang, Haonan |
| collection | NTU |
| description | A liquid crystal (LC)-based optofluidic whispering gallery mode (WGM) resonator has been applied as a biosensor to detect biotin. Immobilized streptavidin (SA) act as protein molecules and specifically bind to biotin through strong non-covalent interaction, which can interfere with the orientation of LCs by decreasing the vertical anchoring force of the alignment layer in which the WGM spectral wavelength shift is monitored as a sensing parameter. Due to the double magnification of the LC molecular orientation transition and the resonance of the WGM, the detection limit for SA can reach 1.25 fM (4.7 × 10-13 g/ml). The measurable concentration of biotin and the wavelength shift of the WGM spectrum have an excellent linearity in the range of 0 to 0.1 pg/ml, which can achieve ultra-low detection limit (0.4 fM), i.e., seven orders of magnitude improvement over conventional polarized optical microscope (POM) method. The proposed optofluidic biosensor is highly reproducible and can be used as an ultrasensitive real-time monitoring biosensor, which will open the door for applications to other receptor and ligand models. |
| first_indexed | 2024-10-01T03:46:10Z |
| format | Journal Article |
| id | ntu-10356/171551 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:46:10Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1715512023-11-03T15:40:29Z Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor Wang, Haonan Xu, Tianhua Wang, Ziyihui Liu, Yize Chen, Huaixu Jiang, Junfeng Liu, Tiegen School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Coenzymes Whispering Gallery Modes A liquid crystal (LC)-based optofluidic whispering gallery mode (WGM) resonator has been applied as a biosensor to detect biotin. Immobilized streptavidin (SA) act as protein molecules and specifically bind to biotin through strong non-covalent interaction, which can interfere with the orientation of LCs by decreasing the vertical anchoring force of the alignment layer in which the WGM spectral wavelength shift is monitored as a sensing parameter. Due to the double magnification of the LC molecular orientation transition and the resonance of the WGM, the detection limit for SA can reach 1.25 fM (4.7 × 10-13 g/ml). The measurable concentration of biotin and the wavelength shift of the WGM spectrum have an excellent linearity in the range of 0 to 0.1 pg/ml, which can achieve ultra-low detection limit (0.4 fM), i.e., seven orders of magnitude improvement over conventional polarized optical microscope (POM) method. The proposed optofluidic biosensor is highly reproducible and can be used as an ultrasensitive real-time monitoring biosensor, which will open the door for applications to other receptor and ligand models. Published version National Natural Science Foundation of China (61735011); Horizon 2020 Framework Programme (101008280); Royal Society (IES\R3\223068); China Scholarship Council (202006250152). 2023-10-30T07:16:04Z 2023-10-30T07:16:04Z 2023 Journal Article Wang, H., Xu, T., Wang, Z., Liu, Y., Chen, H., Jiang, J. & Liu, T. (2023). Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor. Biomedical Optics Express, 14(7), 3763-3774. https://dx.doi.org/10.1364/BOE.494783 2156-7085 https://hdl.handle.net/10356/171551 10.1364/BOE.494783 37497519 2-s2.0-85166476829 7 14 3763 3774 en Biomedical Optics Express © 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement. application/pdf |
| spellingShingle | Engineering::Electrical and electronic engineering Coenzymes Whispering Gallery Modes Wang, Haonan Xu, Tianhua Wang, Ziyihui Liu, Yize Chen, Huaixu Jiang, Junfeng Liu, Tiegen Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title | Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title_full | Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title_fullStr | Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title_full_unstemmed | Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title_short | Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor |
| title_sort | highly sensitive and label free detection of biotin using a liquid crystal based optofluidic biosensor |
| topic | Engineering::Electrical and electronic engineering Coenzymes Whispering Gallery Modes |
| url | https://hdl.handle.net/10356/171551 |
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