Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting
Among the different bioprinting techniques, the drop-on-demand (DOD) jetting-based bioprinting approach facilitates contactless deposition of pico/nanoliter droplets of materials and cells for optimal cell‒matrix and cell‒cell interactions. Although bioinks play a critical role in the bioprinting pr...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2023
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| Online Access: | https://hdl.handle.net/10356/171737 |
| _version_ | 1811691380959870976 |
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| author | Ng, Wei Long Huang, Xi Shkolnikov, Viktor Suntornnond, Ratima Yeong, Wai Yee |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Ng, Wei Long Huang, Xi Shkolnikov, Viktor Suntornnond, Ratima Yeong, Wai Yee |
| author_sort | Ng, Wei Long |
| collection | NTU |
| description | Among the different bioprinting techniques, the drop-on-demand (DOD) jetting-based bioprinting approach facilitates contactless deposition of pico/nanoliter droplets of materials and cells for optimal cell‒matrix and cell‒cell interactions. Although bioinks play a critical role in the bioprinting process, there is a poor understanding of the influence of bioink properties on printing performance (such as filament elongation, formation of satellite droplets, and droplet splashing) and cell health (cell viability and proliferation) during the DOD jetting-based bioprinting process. An inert polyvinylpyrrolidone (PVP360, molecular weight=360 kDa) polymer was used in this study to manipulate the physical properties of the bioinks and investigate the influence of bioink properties on printing performance and cell health. Our experimental results showed that a higher bioink viscoelasticity helps to stabilize droplet filaments before rupturing from the nozzle orifice. The highly stretched droplet filament resulted in the formation of highly aligned “satellite droplets,” which minimized the displacement of the satellite droplets away from the predefined positions. Next, a significant increase in the bioink viscosity facilitated droplet deposition on the wetted substrate surface in the absence of splashing and significantly improved the accuracy of the deposited main droplet. Further analysis showed that cell-laden bioinks with higher viscosity exhibited higher measured average cell viability (%), as the presence of polymer within the printed droplets provides an additional cushioning effect (higher energy dissipation) for the encapsulated cells during droplet impact on the substrate surface, improves the measured average cell viability even at higher droplet impact velocity and retains the proliferation capability of the printed cells. Understanding the influence of bioink properties (e.g., bioink viscoelasticity and viscosity) on printing performance and cell proliferation is important for the formulation of new bioinks, and we have demonstrated precise DOD deposition of living cells and fabrication of tunable cell spheroids (nL‒µL range) using multiple types of cells in a facile manner. Graphic abstract: [Figure not available: see fulltext.]. |
| first_indexed | 2024-10-01T06:18:59Z |
| format | Journal Article |
| id | ntu-10356/171737 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:18:59Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1717372023-11-06T07:41:42Z Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting Ng, Wei Long Huang, Xi Shkolnikov, Viktor Suntornnond, Ratima Yeong, Wai Yee School of Mechanical and Aerospace Engineering HP-NTU Digital Manufacturing Corporate Lab Singapore Centre for 3D Printing Engineering::Mechanical engineering Biofabrication 3D Bioprinting Among the different bioprinting techniques, the drop-on-demand (DOD) jetting-based bioprinting approach facilitates contactless deposition of pico/nanoliter droplets of materials and cells for optimal cell‒matrix and cell‒cell interactions. Although bioinks play a critical role in the bioprinting process, there is a poor understanding of the influence of bioink properties on printing performance (such as filament elongation, formation of satellite droplets, and droplet splashing) and cell health (cell viability and proliferation) during the DOD jetting-based bioprinting process. An inert polyvinylpyrrolidone (PVP360, molecular weight=360 kDa) polymer was used in this study to manipulate the physical properties of the bioinks and investigate the influence of bioink properties on printing performance and cell health. Our experimental results showed that a higher bioink viscoelasticity helps to stabilize droplet filaments before rupturing from the nozzle orifice. The highly stretched droplet filament resulted in the formation of highly aligned “satellite droplets,” which minimized the displacement of the satellite droplets away from the predefined positions. Next, a significant increase in the bioink viscosity facilitated droplet deposition on the wetted substrate surface in the absence of splashing and significantly improved the accuracy of the deposited main droplet. Further analysis showed that cell-laden bioinks with higher viscosity exhibited higher measured average cell viability (%), as the presence of polymer within the printed droplets provides an additional cushioning effect (higher energy dissipation) for the encapsulated cells during droplet impact on the substrate surface, improves the measured average cell viability even at higher droplet impact velocity and retains the proliferation capability of the printed cells. Understanding the influence of bioink properties (e.g., bioink viscoelasticity and viscosity) on printing performance and cell proliferation is important for the formulation of new bioinks, and we have demonstrated precise DOD deposition of living cells and fabrication of tunable cell spheroids (nL‒µL range) using multiple types of cells in a facile manner. Graphic abstract: [Figure not available: see fulltext.]. This study was supported under the RIE2020 Industry Alignment Fund—Industry Collaboration Projects (IAF-ICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner, HP Inc., through the HP-NTU Digital Manufacturing Corporate Lab. 2023-11-06T07:41:41Z 2023-11-06T07:41:41Z 2023 Journal Article Ng, W. L., Huang, X., Shkolnikov, V., Suntornnond, R. & Yeong, W. Y. (2023). Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting. Bio-Design and Manufacturing. https://dx.doi.org/10.1007/s42242-023-00245-3 2096-5524 https://hdl.handle.net/10356/171737 10.1007/s42242-023-00245-3 2-s2.0-85162005156 en IAF-ICP Bio-Design and Manufacturing © 2023 Zhejiang University Press. All rights reserved. |
| spellingShingle | Engineering::Mechanical engineering Biofabrication 3D Bioprinting Ng, Wei Long Huang, Xi Shkolnikov, Viktor Suntornnond, Ratima Yeong, Wai Yee Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title | Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title_full | Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title_fullStr | Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title_full_unstemmed | Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title_short | Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting |
| title_sort | polyvinylpyrrolidone based bioink influence of bioink properties on printing performance and cell proliferation during inkjet based bioprinting |
| topic | Engineering::Mechanical engineering Biofabrication 3D Bioprinting |
| url | https://hdl.handle.net/10356/171737 |
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