Kinetic control of shape deformations and membrane phase separation inside giant vesicles
A variety of cellular processes use liquid-liquid phase separation (LLPS) to create functional levels of organization, but the kinetic pathways by which it proceeds remain incompletely understood. Here in real time, we monitor the dynamics of LLPS of mixtures of segregatively phase-separating polyme...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/171799 |
| _version_ | 1811679178925277184 |
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| author | Su, Wan-Chih Ho, James Chin Shing Gettel, Douglas L. Rowland, Andrew T. Keating, Christine D. Parikh, Atul N. |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Su, Wan-Chih Ho, James Chin Shing Gettel, Douglas L. Rowland, Andrew T. Keating, Christine D. Parikh, Atul N. |
| author_sort | Su, Wan-Chih |
| collection | NTU |
| description | A variety of cellular processes use liquid-liquid phase separation (LLPS) to create functional levels of organization, but the kinetic pathways by which it proceeds remain incompletely understood. Here in real time, we monitor the dynamics of LLPS of mixtures of segregatively phase-separating polymers inside all-synthetic, giant unilamellar vesicles. After dynamically triggering phase separation, we find that the ensuing relaxation-en route to the new equilibrium-is non-trivially modulated by a dynamic interplay between the coarsening of the evolving droplet phase and the interactive membrane boundary. The membrane boundary is preferentially wetted by one of the incipient phases, dynamically arresting the progression of coarsening and deforming the membrane. When the vesicles are composed of phase-separating mixtures of common lipids, LLPS within the vesicular interior becomes coupled to the membrane's compositional degrees of freedom, producing microphase-separated membrane textures. This coupling of bulk and surface phase-separation processes suggests a physical principle by which LLPS inside living cells might be dynamically regulated and communicated to the cellular boundaries. |
| first_indexed | 2024-10-01T03:05:02Z |
| format | Journal Article |
| id | ntu-10356/171799 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:02Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1717992023-11-08T03:02:42Z Kinetic control of shape deformations and membrane phase separation inside giant vesicles Su, Wan-Chih Ho, James Chin Shing Gettel, Douglas L. Rowland, Andrew T. Keating, Christine D. Parikh, Atul N. Singapore Centre for Environmental Life Sciences and Engineering Institute For Digital Molecular Analytics and Science Engineering::Chemical engineering Spinodal Decomposition Domain Growth A variety of cellular processes use liquid-liquid phase separation (LLPS) to create functional levels of organization, but the kinetic pathways by which it proceeds remain incompletely understood. Here in real time, we monitor the dynamics of LLPS of mixtures of segregatively phase-separating polymers inside all-synthetic, giant unilamellar vesicles. After dynamically triggering phase separation, we find that the ensuing relaxation-en route to the new equilibrium-is non-trivially modulated by a dynamic interplay between the coarsening of the evolving droplet phase and the interactive membrane boundary. The membrane boundary is preferentially wetted by one of the incipient phases, dynamically arresting the progression of coarsening and deforming the membrane. When the vesicles are composed of phase-separating mixtures of common lipids, LLPS within the vesicular interior becomes coupled to the membrane's compositional degrees of freedom, producing microphase-separated membrane textures. This coupling of bulk and surface phase-separation processes suggests a physical principle by which LLPS inside living cells might be dynamically regulated and communicated to the cellular boundaries. Nanyang Technological University W.-C.S., D.L.G. and A.N.P. acknowledge funding from the National Science Foundation (DMR-1810540). J.C.S.H. and A.N.P. acknowledge funding and support from the Singapore Centre for Environmental Life Sciences Engineering and the Institute for Digital Molecular Analytics and Science, Nanyang Technological University. C.D.K. and A.T.R. were supported by the US Department of Energy, Office of Science, Basic Energy Sciences under award no. DE-SC0008633. 2023-11-08T03:02:42Z 2023-11-08T03:02:42Z 2023 Journal Article Su, W., Ho, J. C. S., Gettel, D. L., Rowland, A. T., Keating, C. D. & Parikh, A. N. (2023). Kinetic control of shape deformations and membrane phase separation inside giant vesicles. Nature Chemistry. https://dx.doi.org/10.1038/s41557-023-01267-1 1755-4330 https://hdl.handle.net/10356/171799 10.1038/s41557-023-01267-1 37414881 2-s2.0-85164110116 en Nature Chemistry © 2023 The Author(s), under exclusive licence to Springer Nature Limited. All rights reserved |
| spellingShingle | Engineering::Chemical engineering Spinodal Decomposition Domain Growth Su, Wan-Chih Ho, James Chin Shing Gettel, Douglas L. Rowland, Andrew T. Keating, Christine D. Parikh, Atul N. Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title | Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title_full | Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title_fullStr | Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title_full_unstemmed | Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title_short | Kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| title_sort | kinetic control of shape deformations and membrane phase separation inside giant vesicles |
| topic | Engineering::Chemical engineering Spinodal Decomposition Domain Growth |
| url | https://hdl.handle.net/10356/171799 |
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