The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification
The application of separation technology using ultrafiltration/nanofiltration membranes for protein purification and concentration has grown rapidly in the last decade. Innovations to synthesize membranes with properties and performance that suit the characteristics of the feed solution have been an...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/171871 |
| _version_ | 1826115844693819392 |
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| author | Arahman, Nasrul Sari, Widia Puspita Sari, Indah Maulana Rosnelly, Cut Meurah Mulyati, Sri Fahrina, Afrillia Bilad, Muhammad Roil Gunawan, Poernomo Pasaoglu, Mehmet Emin Teber, Oğuz Orhun Vatanpour, Vahid Koyuncu, Ismail Yusni, Yusni |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Arahman, Nasrul Sari, Widia Puspita Sari, Indah Maulana Rosnelly, Cut Meurah Mulyati, Sri Fahrina, Afrillia Bilad, Muhammad Roil Gunawan, Poernomo Pasaoglu, Mehmet Emin Teber, Oğuz Orhun Vatanpour, Vahid Koyuncu, Ismail Yusni, Yusni |
| author_sort | Arahman, Nasrul |
| collection | NTU |
| description | The application of separation technology using ultrafiltration/nanofiltration membranes for protein purification and concentration has grown rapidly in the last decade. Innovations to synthesize membranes with properties and performance that suit the characteristics of the feed solution have been and will keep developing. This study aims to examine the strategies to improve the performance of the Polyvinylidene Fluoride (PVDF) membrane for lactalbumin protein isolation. The PVDF polymer membrane was modified by adding Poloxamer 188 (Po1) copolymer and a combination of two types of nanocarbons, i.e., single-walled carbon nanotubes (S-CnT) and multi-walled carbon nanotubes (M-CnT). The following membrane characteristics were examined: mechanical properties, morphological structure, porosity, elemental composition and functional groups, and surface hydrophilicity. The membrane’s filtration performance was analyzed in terms of its ability to pass water (flux) and concentrate lactalbumin protein. The results showed that the changes in the membrane morphological structure were clearly visible in the SEM test, which exposed more open membrane pores after adding Pol and S-CnT/M-CnT additives. The mechanical properties of the membrane also increased, as indicated by the increase in the tensile strength from 12.1 MPa to 16.07 MPa. In general, it was found that the composition of the PVDF/Pol/S-CnT/NMP polymer solutions resulted in better filtration performance compared to the membranes made of only the PVDF/NMP polymer solution. |
| first_indexed | 2024-10-01T04:01:46Z |
| format | Journal Article |
| id | ntu-10356/171871 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:01:46Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1718712023-12-29T06:47:18Z The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification Arahman, Nasrul Sari, Widia Puspita Sari, Indah Maulana Rosnelly, Cut Meurah Mulyati, Sri Fahrina, Afrillia Bilad, Muhammad Roil Gunawan, Poernomo Pasaoglu, Mehmet Emin Teber, Oğuz Orhun Vatanpour, Vahid Koyuncu, Ismail Yusni, Yusni School of Chemical and Biomedical Engineering Engineering::Chemical engineering Ultrafiltration Membranes Carbon Nanotube The application of separation technology using ultrafiltration/nanofiltration membranes for protein purification and concentration has grown rapidly in the last decade. Innovations to synthesize membranes with properties and performance that suit the characteristics of the feed solution have been and will keep developing. This study aims to examine the strategies to improve the performance of the Polyvinylidene Fluoride (PVDF) membrane for lactalbumin protein isolation. The PVDF polymer membrane was modified by adding Poloxamer 188 (Po1) copolymer and a combination of two types of nanocarbons, i.e., single-walled carbon nanotubes (S-CnT) and multi-walled carbon nanotubes (M-CnT). The following membrane characteristics were examined: mechanical properties, morphological structure, porosity, elemental composition and functional groups, and surface hydrophilicity. The membrane’s filtration performance was analyzed in terms of its ability to pass water (flux) and concentrate lactalbumin protein. The results showed that the changes in the membrane morphological structure were clearly visible in the SEM test, which exposed more open membrane pores after adding Pol and S-CnT/M-CnT additives. The mechanical properties of the membrane also increased, as indicated by the increase in the tensile strength from 12.1 MPa to 16.07 MPa. In general, it was found that the composition of the PVDF/Pol/S-CnT/NMP polymer solutions resulted in better filtration performance compared to the membranes made of only the PVDF/NMP polymer solution. Published version This research was funded by the Indonesian Ministry of Education, Culture, Research, and Technology with grant contract number 46/UN11.2.1/PT.01.03/DRPM/2022 (Penelitian Dasar Unggulan Perguruan Tinggi). 2023-11-14T01:51:56Z 2023-11-14T01:51:56Z 2022 Journal Article Arahman, N., Sari, W. P., Sari, I. M., Rosnelly, C. M., Mulyati, S., Fahrina, A., Bilad, M. R., Gunawan, P., Pasaoglu, M. E., Teber, O. O., Vatanpour, V., Koyuncu, I. & Yusni, Y. (2022). The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification. ChemEngineering, 6(6), 88-. https://dx.doi.org/10.3390/chemengineering6060088 2305-7084 https://hdl.handle.net/10356/171871 10.3390/chemengineering6060088 2-s2.0-85144713356 6 6 88 en ChemEngineering © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Engineering::Chemical engineering Ultrafiltration Membranes Carbon Nanotube Arahman, Nasrul Sari, Widia Puspita Sari, Indah Maulana Rosnelly, Cut Meurah Mulyati, Sri Fahrina, Afrillia Bilad, Muhammad Roil Gunawan, Poernomo Pasaoglu, Mehmet Emin Teber, Oğuz Orhun Vatanpour, Vahid Koyuncu, Ismail Yusni, Yusni The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title | The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title_full | The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title_fullStr | The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title_full_unstemmed | The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title_short | The effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single/multi-walled carbon nanotubes for lactalbumin purification |
| title_sort | effectiveness of polyvinylidene fluoride membranes modified with poloxamer and single multi walled carbon nanotubes for lactalbumin purification |
| topic | Engineering::Chemical engineering Ultrafiltration Membranes Carbon Nanotube |
| url | https://hdl.handle.net/10356/171871 |
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