Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic
In this study, we report the implementation of a comprehensive wastewater surveillance testing program at a university campus in Singapore to identify Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected individuals and the usage of pharmaceuticals and personal care products (PPCPs)...
| 主要な著者: | , , , , , , , , , , , , , , , |
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| その他の著者: | |
| フォーマット: | Journal Article |
| 言語: | English |
| 出版事項: |
2023
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| 主題: | |
| オンライン･アクセス: | https://hdl.handle.net/10356/172447 |
| _version_ | 1826120577550647296 |
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| author | Mohapatra, Sanjeeb Bhatia, Sumedha Senaratna, Kavindra Yohan Kuhatheva Jong, Mui-Choo Lim, Benjamin Chun Min Gangesh, G. Reuben Lee, Jia Xiong Goh, Shin Giek Cheung, Callie Lin, Yutao You, Luhua Ng, How Yong Lim, Cheh Peng Wong, Judith Chui Ching Ng, Lee Ching Gin, Karina Yew-Hoong |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Mohapatra, Sanjeeb Bhatia, Sumedha Senaratna, Kavindra Yohan Kuhatheva Jong, Mui-Choo Lim, Benjamin Chun Min Gangesh, G. Reuben Lee, Jia Xiong Goh, Shin Giek Cheung, Callie Lin, Yutao You, Luhua Ng, How Yong Lim, Cheh Peng Wong, Judith Chui Ching Ng, Lee Ching Gin, Karina Yew-Hoong |
| author_sort | Mohapatra, Sanjeeb |
| collection | NTU |
| description | In this study, we report the implementation of a comprehensive wastewater surveillance testing program at a university campus in Singapore to identify Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected individuals and the usage of pharmaceuticals and personal care products (PPCPs) as well as other emerging contaminants (ECs). This unique co-monitoring program simultaneously measured SARS-CoV-2 with chemical markers/contaminants as the COVID-19 situation evolved from pandemic to endemic stages, following a nationwide mass vaccination drive. SARS-CoV-2 RNA concentrations in wastewater from campus dormitories were measured using real-time reverse transcription-polymerase chain reaction (RT-qPCR) and corroborated with the number of symptomatic COVID-19 cases confirmed with the antigen rapid test (ART). Consistent results were observed where the concentrations of SARS-CoV-2 RNA detected in wastewater increased proportionately with the number of COVID-19 infected individuals residing on campus. Similarly, a wide range of ECs, including disinfectants and antibiotics, were detected through sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) techniques to establish PPCPs consumption patterns during various stages of the COVID-19 pandemic in Singapore. Statistical correlation of SARS-CoV-2 RNA was observed with few ECs belonging to disinfectants, PCPs and antibiotics. A high concentration of disinfectants and subsequent positive correlation with the number of reported cases on the university campus indicates that disinfectants could serve as a chemical marker during such unprecedented times. |
| first_indexed | 2024-10-01T05:18:45Z |
| format | Journal Article |
| id | ntu-10356/172447 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:18:45Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1724472023-12-11T15:31:59Z Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic Mohapatra, Sanjeeb Bhatia, Sumedha Senaratna, Kavindra Yohan Kuhatheva Jong, Mui-Choo Lim, Benjamin Chun Min Gangesh, G. Reuben Lee, Jia Xiong Goh, Shin Giek Cheung, Callie Lin, Yutao You, Luhua Ng, How Yong Lim, Cheh Peng Wong, Judith Chui Ching Ng, Lee Ching Gin, Karina Yew-Hoong School of Biological Sciences National Environment Agency Science::Biological sciences Wastewater-Based Epidemiology University Campus In this study, we report the implementation of a comprehensive wastewater surveillance testing program at a university campus in Singapore to identify Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected individuals and the usage of pharmaceuticals and personal care products (PPCPs) as well as other emerging contaminants (ECs). This unique co-monitoring program simultaneously measured SARS-CoV-2 with chemical markers/contaminants as the COVID-19 situation evolved from pandemic to endemic stages, following a nationwide mass vaccination drive. SARS-CoV-2 RNA concentrations in wastewater from campus dormitories were measured using real-time reverse transcription-polymerase chain reaction (RT-qPCR) and corroborated with the number of symptomatic COVID-19 cases confirmed with the antigen rapid test (ART). Consistent results were observed where the concentrations of SARS-CoV-2 RNA detected in wastewater increased proportionately with the number of COVID-19 infected individuals residing on campus. Similarly, a wide range of ECs, including disinfectants and antibiotics, were detected through sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) techniques to establish PPCPs consumption patterns during various stages of the COVID-19 pandemic in Singapore. Statistical correlation of SARS-CoV-2 RNA was observed with few ECs belonging to disinfectants, PCPs and antibiotics. A high concentration of disinfectants and subsequent positive correlation with the number of reported cases on the university campus indicates that disinfectants could serve as a chemical marker during such unprecedented times. National Environmental Agency (NEA) National Research Foundation (NRF) Published version This research is co-funded by the National University of Singapore (NUS), National Environment Agency (NEA) and the National Research Foundation (NRF), Prime Minister’s Office, Singapore under its Campus for Research Excellence and Technological Enterprise (CREATE) program (Intra-CREATE Thematic Project NRF2019-THE001-0003), administered by the NUS Environmental Research Institute (NERI), Singapore. 2023-12-11T02:18:22Z 2023-12-11T02:18:22Z 2023 Journal Article Mohapatra, S., Bhatia, S., Senaratna, K. Y. K., Jong, M., Lim, B. C. M., Gangesh, G. R., Lee, J. X., Goh, S. G., Cheung, C., Lin, Y., You, L., Ng, H. Y., Lim, C. P., Wong, J. C. C., Ng, L. C. & Gin, K. Y. (2023). Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic. Journal of Hazardous Materials, 446, 130690-. https://dx.doi.org/10.1016/j.jhazmat.2022.130690 0304-3894 https://hdl.handle.net/10356/172447 10.1016/j.jhazmat.2022.130690 36603423 2-s2.0-85146032350 446 130690 en NRF2019-THE001-0003 Journal of Hazardous Materials © 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Wastewater-Based Epidemiology University Campus Mohapatra, Sanjeeb Bhatia, Sumedha Senaratna, Kavindra Yohan Kuhatheva Jong, Mui-Choo Lim, Benjamin Chun Min Gangesh, G. Reuben Lee, Jia Xiong Goh, Shin Giek Cheung, Callie Lin, Yutao You, Luhua Ng, How Yong Lim, Cheh Peng Wong, Judith Chui Ching Ng, Lee Ching Gin, Karina Yew-Hoong Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title | Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title_full | Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title_fullStr | Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title_full_unstemmed | Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title_short | Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID - 19 pandemic |
| title_sort | wastewater surveillance of sars cov 2 and chemical markers in campus dormitories in an evolving covid 19 pandemic |
| topic | Science::Biological sciences Wastewater-Based Epidemiology University Campus |
| url | https://hdl.handle.net/10356/172447 |
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