Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection
Flow cytometry is an essential technique in single particle analysis and cell sorting for further downstream diagnosis, exhibiting high-throughput and multiplexing capabilities for many biological and biomedical applications. Although many hydrodynamic focusing-based microfluidic cytometers have bee...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/173371 |
| _version_ | 1826120971868700672 |
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| author | Zhao, Wenhan Shang, Xiaopeng Zhang, Boran Yuan, Dan Nguyen, Binh Thi Thanh Wu, Wenshuai Zhang, Jing Bo Peng, Niancai Liu, Ai Qun Duan, Fei Chin, Lip Ket |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Zhao, Wenhan Shang, Xiaopeng Zhang, Boran Yuan, Dan Nguyen, Binh Thi Thanh Wu, Wenshuai Zhang, Jing Bo Peng, Niancai Liu, Ai Qun Duan, Fei Chin, Lip Ket |
| author_sort | Zhao, Wenhan |
| collection | NTU |
| description | Flow cytometry is an essential technique in single particle analysis and cell sorting for further downstream diagnosis, exhibiting high-throughput and multiplexing capabilities for many biological and biomedical applications. Although many hydrodynamic focusing-based microfluidic cytometers have been demonstrated with reduced size and cost to adapt to point-of-care settings, the operating conditions are not characterized systematically. This study presents the flow transition process in the hydrodynamic focusing mechanism when the flow rate or the Reynolds number increases. The characteristics of flow fields and mass transport were studied under various operating conditions, including flow rates and microchannel heights. A transition from the squeezed focusing state to the over-squeezed anti-focusing state in the hydrodynamic focusing regime was observed when the Reynolds number increased above 30. Parametric studies illustrated that the focusing width increased with the Reynolds number but decreased with the microchannel height in the over-squeezed state. The microfluidic cytometric analyses using microbeads and E. coli show that the recovery rate was maintained by limiting the Reynolds number to 30. The detailed analysis of the flow transition will provide new insight into microfluidic cytometric analyses with a broad range of applications in food safety, water monitoring and healthcare sectors. |
| first_indexed | 2024-10-01T05:25:09Z |
| format | Journal Article |
| id | ntu-10356/173371 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:25:09Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1733712024-01-30T04:32:50Z Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection Zhao, Wenhan Shang, Xiaopeng Zhang, Boran Yuan, Dan Nguyen, Binh Thi Thanh Wu, Wenshuai Zhang, Jing Bo Peng, Niancai Liu, Ai Qun Duan, Fei Chin, Lip Ket School of Electrical and Electronic Engineering School of Mechanical and Aerospace Engineering Engineering::Electrical and electronic engineering Engineering::Mechanical engineering Bacteria Detection Cytometric Analysis Flow cytometry is an essential technique in single particle analysis and cell sorting for further downstream diagnosis, exhibiting high-throughput and multiplexing capabilities for many biological and biomedical applications. Although many hydrodynamic focusing-based microfluidic cytometers have been demonstrated with reduced size and cost to adapt to point-of-care settings, the operating conditions are not characterized systematically. This study presents the flow transition process in the hydrodynamic focusing mechanism when the flow rate or the Reynolds number increases. The characteristics of flow fields and mass transport were studied under various operating conditions, including flow rates and microchannel heights. A transition from the squeezed focusing state to the over-squeezed anti-focusing state in the hydrodynamic focusing regime was observed when the Reynolds number increased above 30. Parametric studies illustrated that the focusing width increased with the Reynolds number but decreased with the microchannel height in the over-squeezed state. The microfluidic cytometric analyses using microbeads and E. coli show that the recovery rate was maintained by limiting the Reynolds number to 30. The detailed analysis of the flow transition will provide new insight into microfluidic cytometric analyses with a broad range of applications in food safety, water monitoring and healthcare sectors. Public Utilities Board (PUB) This work was supported by the Competitive Research Program Water Project of Singapore (A. Q. Liu, PUB-1804-0082) and the City University of Hong Kong (L. K. Chin, 9610572). 2024-01-30T04:32:50Z 2024-01-30T04:32:50Z 2023 Journal Article Zhao, W., Shang, X., Zhang, B., Yuan, D., Nguyen, B. T. T., Wu, W., Zhang, J. B., Peng, N., Liu, A. Q., Duan, F. & Chin, L. K. (2023). Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection. Lab On a Chip, 23(23), 5039-5046. https://dx.doi.org/10.1039/d3lc00434a 1473-0197 https://hdl.handle.net/10356/173371 10.1039/d3lc00434a 37909299 2-s2.0-85176137018 23 23 5039 5046 en PUB-1804-0082 Lab on a Chip © 2023 The Authors. All rights reserved. |
| spellingShingle | Engineering::Electrical and electronic engineering Engineering::Mechanical engineering Bacteria Detection Cytometric Analysis Zhao, Wenhan Shang, Xiaopeng Zhang, Boran Yuan, Dan Nguyen, Binh Thi Thanh Wu, Wenshuai Zhang, Jing Bo Peng, Niancai Liu, Ai Qun Duan, Fei Chin, Lip Ket Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title | Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title_full | Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title_fullStr | Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title_full_unstemmed | Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title_short | Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection |
| title_sort | squeezed state in the hydrodynamic focusing regime for escherichia coli bacteria detection |
| topic | Engineering::Electrical and electronic engineering Engineering::Mechanical engineering Bacteria Detection Cytometric Analysis |
| url | https://hdl.handle.net/10356/173371 |
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