Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia
Objectives: To explore the association of recent hospitalization and asymptomatic carriage of multidrug-resistant Enterobacterales (MDRE) and determine the prevailing strains and antibiotic resistance genes in Siem Reap, Cambodia using WGS. Methods: In this cross-sectional study, faecal samples were...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/173553 |
| _version_ | 1811676675799252992 |
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| author | Singh, Shweta R. Tang, Cheng Yee Mao, Bunsoth Soeng, Sona Ling, Clare L. Teo, Jocelyn Qi-Min Vonthanak, Saphonn Turner, Paul Hsu, Li Yang Ong, Rick Twee-Hee |
| author2 | Saw Swee Hock School of Public Health, NUS |
| author_facet | Saw Swee Hock School of Public Health, NUS Singh, Shweta R. Tang, Cheng Yee Mao, Bunsoth Soeng, Sona Ling, Clare L. Teo, Jocelyn Qi-Min Vonthanak, Saphonn Turner, Paul Hsu, Li Yang Ong, Rick Twee-Hee |
| author_sort | Singh, Shweta R. |
| collection | NTU |
| description | Objectives: To explore the association of recent hospitalization and asymptomatic carriage of multidrug-resistant Enterobacterales (MDRE) and determine the prevailing strains and antibiotic resistance genes in Siem Reap, Cambodia using WGS. Methods: In this cross-sectional study, faecal samples were collected from two arms: a hospital-associated arm consisted of recently hospitalized children (2–14 years), with their family members; and a community-associated arm comprising children in the matching age group and their family members with no recent hospitalization. Forty-two families in each study arm were recruited, with 376 enrolled participants (169 adults and 207 children) and 290 stool specimens collected from participants. The DNA of ESBL- and carbapenemase-producing Enterobacterales cultured from the faecal samples was subject to WGS on the Illumina NovaSeq platform. Results: Of the 290 stool specimens, 277 Escherichia coli isolates and 130 Klebsiella spp. were identified on CHROMagar ESBL and KPC plates. The DNA of 276 E. coli (one isolate failed quality control test), 89 Klebsiella pneumoniae, 40 Klebsiella quasipneumoniae and 1 Klebsiella variicola was sequenced. CTX-M-15 was the most common ESBL gene found in E. coli (n=104, 38%), K. pneumoniae (n=50, 56%) and K. quasipneumoniae (n=16, 40%). The prevalence of bacterial lineages and ESBL genes was not associated with any specific arm. Conclusions: Our results demonstrate that MDRE is likely to be endemic within the Siem Reap community. ESBL genes, specifically blaCTX-M, can be found in almost all E. coli commensals, indicating that these genes are continuously propagated in the community through various unknown channels at present. |
| first_indexed | 2024-10-01T02:25:15Z |
| format | Journal Article |
| id | ntu-10356/173553 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:25:15Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1735532024-02-15T15:30:21Z Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia Singh, Shweta R. Tang, Cheng Yee Mao, Bunsoth Soeng, Sona Ling, Clare L. Teo, Jocelyn Qi-Min Vonthanak, Saphonn Turner, Paul Hsu, Li Yang Ong, Rick Twee-Hee Saw Swee Hock School of Public Health, NUS Yong Loo Lin School of Medicine, NUS Singapore Centre for Environmental Life Sciences and Engineering Medicine, Health and Life Sciences Antibiotic Resistance Cambodia Objectives: To explore the association of recent hospitalization and asymptomatic carriage of multidrug-resistant Enterobacterales (MDRE) and determine the prevailing strains and antibiotic resistance genes in Siem Reap, Cambodia using WGS. Methods: In this cross-sectional study, faecal samples were collected from two arms: a hospital-associated arm consisted of recently hospitalized children (2–14 years), with their family members; and a community-associated arm comprising children in the matching age group and their family members with no recent hospitalization. Forty-two families in each study arm were recruited, with 376 enrolled participants (169 adults and 207 children) and 290 stool specimens collected from participants. The DNA of ESBL- and carbapenemase-producing Enterobacterales cultured from the faecal samples was subject to WGS on the Illumina NovaSeq platform. Results: Of the 290 stool specimens, 277 Escherichia coli isolates and 130 Klebsiella spp. were identified on CHROMagar ESBL and KPC plates. The DNA of 276 E. coli (one isolate failed quality control test), 89 Klebsiella pneumoniae, 40 Klebsiella quasipneumoniae and 1 Klebsiella variicola was sequenced. CTX-M-15 was the most common ESBL gene found in E. coli (n=104, 38%), K. pneumoniae (n=50, 56%) and K. quasipneumoniae (n=16, 40%). The prevalence of bacterial lineages and ESBL genes was not associated with any specific arm. Conclusions: Our results demonstrate that MDRE is likely to be endemic within the Siem Reap community. ESBL genes, specifically blaCTX-M, can be found in almost all E. coli commensals, indicating that these genes are continuously propagated in the community through various unknown channels at present. Ministry of Education (MOE) Ministry of Health (MOH) National Medical Research Council (NMRC) Published version This work was funded by the Ministry of Education, Singapore under the Academic Research Fund Tier 1 (FY2019) and supported by the Singapore Ministry of Health's National Medical Research Council under its Centre Grant Programme (MOH-001010-00). This research was funded in whole, or in part, by the Wellcome Trust (grant number 220211). 2024-02-14T00:54:29Z 2024-02-14T00:54:29Z 2023 Journal Article Singh, S. R., Tang, C. Y., Mao, B., Soeng, S., Ling, C. L., Teo, J. Q., Vonthanak, S., Turner, P., Hsu, L. Y. & Ong, R. T. (2023). Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia. JAC-Antimicrobial Resistance, 5(3). https://dx.doi.org/10.1093/jacamr/dlad067 2632-1823 https://hdl.handle.net/10356/173553 10.1093/jacamr/dlad067 37325250 2-s2.0-85163520083 3 5 en FY2019 MOH-001010-00 JAC-Antimicrobial Resistance © The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Antibiotic Resistance Cambodia Singh, Shweta R. Tang, Cheng Yee Mao, Bunsoth Soeng, Sona Ling, Clare L. Teo, Jocelyn Qi-Min Vonthanak, Saphonn Turner, Paul Hsu, Li Yang Ong, Rick Twee-Hee Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title | Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title_full | Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title_fullStr | Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title_full_unstemmed | Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title_short | Whole genome sequencing of multidrug resistant Enterobacterales identified in children and their household members within Siem Reap, Cambodia |
| title_sort | whole genome sequencing of multidrug resistant enterobacterales identified in children and their household members within siem reap cambodia |
| topic | Medicine, Health and Life Sciences Antibiotic Resistance Cambodia |
| url | https://hdl.handle.net/10356/173553 |
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