Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP
Aeromonas veronii can adhere to host cells through different adherence factors including outer-membrane proteins (OMPs), lipopolysaccharide (LPS), and pili, but its adherence mechanisms are still unclear. Here, we evaluated the effect of autoinducer-2 (AI-2) on adherence of A. veronii and its regula...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/174041 |
| _version_ | 1811683236149985280 |
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| author | Li, Yi Han, Shuo Wang, Yuqi Qin, Mengyuan Lu, Chengjin Ma, Yingke Yang, Wenqing Liu, Jiajia Xia, Xiaohua Wang, Hailei |
| author2 | Nanyang Environment and Water Research Institute |
| author_facet | Nanyang Environment and Water Research Institute Li, Yi Han, Shuo Wang, Yuqi Qin, Mengyuan Lu, Chengjin Ma, Yingke Yang, Wenqing Liu, Jiajia Xia, Xiaohua Wang, Hailei |
| author_sort | Li, Yi |
| collection | NTU |
| description | Aeromonas veronii can adhere to host cells through different adherence factors including outer-membrane proteins (OMPs), lipopolysaccharide (LPS), and pili, but its adherence mechanisms are still unclear. Here, we evaluated the effect of autoinducer-2 (AI-2) on adherence of A. veronii and its regulation mechanism. After determination of the promotion effect of AI-2 on adherence, we investigated which adherence factor was regulated by AI-2, and the results show that AI-2 only limits the formation of pili. Among the four distinct pili systems, only the mannose-sensitive hemagglutinin (MSHA) type IV pili genes were significantly downregulated after deficiency of AI-2. MshE, an ATPase belonged to MSHA type IV pilin, was confirmed as c-di-GMP receptor, that can bind with c-di-GMP which is positively regulated by AI-2, and the increase of c-di-GMP can promote the expression of MSHA type IV pili genes and adherence of A. veronii. Therefore, this study confirms that c-di-GMP positively regulated by AI-2 binds with MshE, then increases the expression of MSHA pili genes, finally promoting adherence of A. veronii, suggesting a multilevel positive regulatory adhesion mechanism that is responsible for A. veronii adherence. |
| first_indexed | 2024-10-01T04:09:31Z |
| format | Journal Article |
| id | ntu-10356/174041 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:09:31Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1740412024-03-13T15:34:36Z Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP Li, Yi Han, Shuo Wang, Yuqi Qin, Mengyuan Lu, Chengjin Ma, Yingke Yang, Wenqing Liu, Jiajia Xia, Xiaohua Wang, Hailei Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Center Earth and Environmental Sciences Aeromonas veronii Autoinducer-2 Aeromonas veronii can adhere to host cells through different adherence factors including outer-membrane proteins (OMPs), lipopolysaccharide (LPS), and pili, but its adherence mechanisms are still unclear. Here, we evaluated the effect of autoinducer-2 (AI-2) on adherence of A. veronii and its regulation mechanism. After determination of the promotion effect of AI-2 on adherence, we investigated which adherence factor was regulated by AI-2, and the results show that AI-2 only limits the formation of pili. Among the four distinct pili systems, only the mannose-sensitive hemagglutinin (MSHA) type IV pili genes were significantly downregulated after deficiency of AI-2. MshE, an ATPase belonged to MSHA type IV pilin, was confirmed as c-di-GMP receptor, that can bind with c-di-GMP which is positively regulated by AI-2, and the increase of c-di-GMP can promote the expression of MSHA type IV pili genes and adherence of A. veronii. Therefore, this study confirms that c-di-GMP positively regulated by AI-2 binds with MshE, then increases the expression of MSHA pili genes, finally promoting adherence of A. veronii, suggesting a multilevel positive regulatory adhesion mechanism that is responsible for A. veronii adherence. Published version This work is supported by the National Natural Science Foundation of China (32000073), the Key Scientific Research Programs of Henan Education Department (21A180011), and the Guangdong Basic and Applied Basic Research Foundation (2020B1515120012). 2024-03-12T07:38:15Z 2024-03-12T07:38:15Z 2023 Journal Article Li, Y., Han, S., Wang, Y., Qin, M., Lu, C., Ma, Y., Yang, W., Liu, J., Xia, X. & Wang, H. (2023). Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP. Applied and Environmental Microbiology, 89(11), e0081923-. https://dx.doi.org/10.1128/aem.00819-23 0099-2240 https://hdl.handle.net/10356/174041 10.1128/aem.00819-23 37902393 2-s2.0-85178500180 11 89 e0081923 en Applied and Environmental Microbiology © 2023 American Society for Microbiology. All Rights Reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1128/aem.00819-23. application/pdf |
| spellingShingle | Earth and Environmental Sciences Aeromonas veronii Autoinducer-2 Li, Yi Han, Shuo Wang, Yuqi Qin, Mengyuan Lu, Chengjin Ma, Yingke Yang, Wenqing Liu, Jiajia Xia, Xiaohua Wang, Hailei Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title | Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title_full | Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title_fullStr | Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title_full_unstemmed | Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title_short | Autoinducer-2 promotes adherence of Aeromonas veronii through facilitating the expression of MSHA type IV pili genes mediated by c-di-GMP |
| title_sort | autoinducer 2 promotes adherence of aeromonas veronii through facilitating the expression of msha type iv pili genes mediated by c di gmp |
| topic | Earth and Environmental Sciences Aeromonas veronii Autoinducer-2 |
| url | https://hdl.handle.net/10356/174041 |
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