Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells
Meniscus injury is one of the most common sports injuries within the knee joint, which is also a crucial pathogenic factor for osteoarthritis (OA). The current meniscus substitution products are far from able to restore meniscal biofunctions due to the inability to reconstruct the gradient heterogen...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/175603 |
| _version_ | 1826120893264297984 |
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| author | Du, Mingze Liu, Kangze Lai, Huinan Qian, Jin Ai, Liya Zhang, Jiying Yin, Jun Jiang, Dong |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Du, Mingze Liu, Kangze Lai, Huinan Qian, Jin Ai, Liya Zhang, Jiying Yin, Jun Jiang, Dong |
| author_sort | Du, Mingze |
| collection | NTU |
| description | Meniscus injury is one of the most common sports injuries within the knee joint, which is also a crucial pathogenic factor for osteoarthritis (OA). The current meniscus substitution products are far from able to restore meniscal biofunctions due to the inability to reconstruct the gradient heterogeneity of natural meniscus from biological and biomechanical perspectives. Here, inspired by the topology self-induced effect and native meniscus microstructure, we present an innovative tissue-engineered meniscus (TEM) with a unique gradient-sized diamond-pored microstructure (GSDP-TEM) through dual-stage temperature control 3D-printing system based on the mechanical/biocompatibility compatible high Mw poly(ε-caprolactone) (PCL). Biologically, the unique gradient microtopology allows the seeded mesenchymal stem cells with spatially heterogeneous differentiation, triggering gradient transition of the extracellular matrix (ECM) from the inside out. Biomechanically, GSDP-TEM presents excellent circumferential tensile modulus and load transmission ability similar to the natural meniscus. After implantation in rabbit knee, GSDP-TEM induces the regeneration of biomimetic heterogeneous neomeniscus and efficiently alleviates joint degeneration. This study provides an innovative strategy for functional meniscus reconstruction. Topological self-induced cell differentiation and biomechanical property also provides a simple and effective solution for other complex heterogeneous structure reconstructions in the human body and possesses high clinical translational potential. |
| first_indexed | 2024-10-01T05:23:53Z |
| format | Journal Article |
| id | ntu-10356/175603 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:23:53Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1756032024-05-03T15:31:51Z Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells Du, Mingze Liu, Kangze Lai, Huinan Qian, Jin Ai, Liya Zhang, Jiying Yin, Jun Jiang, Dong School of Chemistry, Chemical Engineering and Biotechnology Medicine, Health and Life Sciences Tissue-engineered meniscus Functional reconstruction Meniscus injury is one of the most common sports injuries within the knee joint, which is also a crucial pathogenic factor for osteoarthritis (OA). The current meniscus substitution products are far from able to restore meniscal biofunctions due to the inability to reconstruct the gradient heterogeneity of natural meniscus from biological and biomechanical perspectives. Here, inspired by the topology self-induced effect and native meniscus microstructure, we present an innovative tissue-engineered meniscus (TEM) with a unique gradient-sized diamond-pored microstructure (GSDP-TEM) through dual-stage temperature control 3D-printing system based on the mechanical/biocompatibility compatible high Mw poly(ε-caprolactone) (PCL). Biologically, the unique gradient microtopology allows the seeded mesenchymal stem cells with spatially heterogeneous differentiation, triggering gradient transition of the extracellular matrix (ECM) from the inside out. Biomechanically, GSDP-TEM presents excellent circumferential tensile modulus and load transmission ability similar to the natural meniscus. After implantation in rabbit knee, GSDP-TEM induces the regeneration of biomimetic heterogeneous neomeniscus and efficiently alleviates joint degeneration. This study provides an innovative strategy for functional meniscus reconstruction. Topological self-induced cell differentiation and biomechanical property also provides a simple and effective solution for other complex heterogeneous structure reconstructions in the human body and possesses high clinical translational potential. Published version This work was supported by National Key R&D Program of China (No. 2019YFB1706905), National Natural Science Foundation of China (82072428, 52075482) and Natural Science Foundation of Beijing, China (7212132). 2024-04-30T05:30:20Z 2024-04-30T05:30:20Z 2024 Journal Article Du, M., Liu, K., Lai, H., Qian, J., Ai, L., Zhang, J., Yin, J. & Jiang, D. (2024). Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells. Bioactive Materials, 36, 358-375. https://dx.doi.org/10.1016/j.bioactmat.2024.03.005 2452-199X https://hdl.handle.net/10356/175603 10.1016/j.bioactmat.2024.03.005 38496031 2-s2.0-85187534934 36 358 375 en Bioactive Materials © 2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Tissue-engineered meniscus Functional reconstruction Du, Mingze Liu, Kangze Lai, Huinan Qian, Jin Ai, Liya Zhang, Jiying Yin, Jun Jiang, Dong Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title | Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title_full | Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title_fullStr | Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title_full_unstemmed | Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title_short | Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells |
| title_sort | functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self induction of stem cells |
| topic | Medicine, Health and Life Sciences Tissue-engineered meniscus Functional reconstruction |
| url | https://hdl.handle.net/10356/175603 |
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