A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting
Endosomes are specialized organelles that function in the secretory and endocytic protein sorting pathways. Endocytosed cell surface receptors and transporters destined for lysosomal degradation are sorted into intraluminal vesicles (ILVs) at endosomes by endosomal sorting complexes required for tra...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/175617 |
| _version_ | 1811696672112115712 |
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| author | Suzuki, Sho W. West, Matthew Zhang, Yichen Fan, Jenny S. Roberts, Rachel T. Odorizzi, Greg Emr, Scott D. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Suzuki, Sho W. West, Matthew Zhang, Yichen Fan, Jenny S. Roberts, Rachel T. Odorizzi, Greg Emr, Scott D. |
| author_sort | Suzuki, Sho W. |
| collection | NTU |
| description | Endosomes are specialized organelles that function in the secretory and endocytic protein sorting pathways. Endocytosed cell surface receptors and transporters destined for lysosomal degradation are sorted into intraluminal vesicles (ILVs) at endosomes by endosomal sorting complexes required for transport (ESCRT) proteins. The endosomes (multivesicular bodies, MVBs) then fuse with the lysosome. During endosomal maturation, the number of ILVs increases, but the size of endosomes does not decrease despite the consumption of the limiting membrane during ILV formation. Vesicle-mediated trafficking is thought to provide lipids to support MVB biogenesis. However, we have uncovered an unexpected contribution of a large bridge-like lipid transfer protein, Vps13, in this process. Here, we reveal that Vps13-mediated lipid transfer at ER-endosome contact sites is required for the ESCRT pathway. We propose that Vps13 may play a critical role in supplying lipids to the endosome, ensuring continuous ESCRT-mediated sorting during MVB biogenesis. |
| first_indexed | 2024-10-01T07:43:05Z |
| format | Journal Article |
| id | ntu-10356/175617 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:43:05Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1756172024-05-06T15:32:09Z A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting Suzuki, Sho W. West, Matthew Zhang, Yichen Fan, Jenny S. Roberts, Rachel T. Odorizzi, Greg Emr, Scott D. School of Biological Sciences Medicine, Health and Life Sciences Endocytosis Endosome Endosomes are specialized organelles that function in the secretory and endocytic protein sorting pathways. Endocytosed cell surface receptors and transporters destined for lysosomal degradation are sorted into intraluminal vesicles (ILVs) at endosomes by endosomal sorting complexes required for transport (ESCRT) proteins. The endosomes (multivesicular bodies, MVBs) then fuse with the lysosome. During endosomal maturation, the number of ILVs increases, but the size of endosomes does not decrease despite the consumption of the limiting membrane during ILV formation. Vesicle-mediated trafficking is thought to provide lipids to support MVB biogenesis. However, we have uncovered an unexpected contribution of a large bridge-like lipid transfer protein, Vps13, in this process. Here, we reveal that Vps13-mediated lipid transfer at ER-endosome contact sites is required for the ESCRT pathway. We propose that Vps13 may play a critical role in supplying lipids to the endosome, ensuring continuous ESCRT-mediated sorting during MVB biogenesis. Published version S.W. Suzuki is supported by Osamu Hayaishi Memorial Scholarship for Study Abroad. This work was supported by a Cornell University Research Grant (CU563704) to S.D. 2024-04-30T07:16:05Z 2024-04-30T07:16:05Z 2024 Journal Article Suzuki, S. W., West, M., Zhang, Y., Fan, J. S., Roberts, R. T., Odorizzi, G. & Emr, S. D. (2024). A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting. Journal of Cell Biology, 223(4), e202307094-. https://dx.doi.org/10.1083/jcb.202307094 0021-9525 https://hdl.handle.net/10356/175617 10.1083/jcb.202307094 38319250 2-s2.0-85184456549 4 223 e202307094 en Journal of Cell Biology © 2024 Suzuki et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Endocytosis Endosome Suzuki, Sho W. West, Matthew Zhang, Yichen Fan, Jenny S. Roberts, Rachel T. Odorizzi, Greg Emr, Scott D. A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title | A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title_full | A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title_fullStr | A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title_full_unstemmed | A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title_short | A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting |
| title_sort | role for vps13 mediated lipid transfer at the er endosome contact site in escrt mediated sorting |
| topic | Medicine, Health and Life Sciences Endocytosis Endosome |
| url | https://hdl.handle.net/10356/175617 |
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