Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice
Naringin, a natural flavanone primarily found in citrus fruits, has garnered increased attention due to its recognized antioxidative, anti-inflammatory, and cardioprotective attributes. However, the functions of naringin in regulating energy expenditure are poorly understood. In the present study, w...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2024
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| Online Access: | https://hdl.handle.net/10356/175767 |
| _version_ | 1811691967599345664 |
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| author | Li, Xiaoping Yao, Zhao Qi, Xinyue Cui, Jinling Zhou, Yuliang Tan, Yihong Huang, Xiaojun Ye, Hui |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Li, Xiaoping Yao, Zhao Qi, Xinyue Cui, Jinling Zhou, Yuliang Tan, Yihong Huang, Xiaojun Ye, Hui |
| author_sort | Li, Xiaoping |
| collection | NTU |
| description | Naringin, a natural flavanone primarily found in citrus fruits, has garnered increased attention due to its recognized antioxidative, anti-inflammatory, and cardioprotective attributes. However, the functions of naringin in regulating energy expenditure are poorly understood. In the present study, we observed that twelve weeks of naringin supplementation substantially reshaped the metabolic profile of high-fat diet (HFD)-fed mice, by inhibiting body weight gain, reducing liver weight, and altering body compositions. Notably, naringin exhibited a remarkable capacity to augment whole-body energy expenditure of the tested mice by enhancing the thermogenic activity of brown adipose tissue (BAT) and stimulating browning of inguinal white adipose tissue (iWAT). Furthermore, our results showed naringin supplementation modified gut microbiota composition, specifically increasing the abundance of Bifidobacterium and Lachnospiraceae_bacterium_28-4, while reducing the abundance of Lachnospiraceae_bacterium_DW59 and Dubosiella_newyorkensis. Subsequently, we also found naringin supplementation altered fecal metabolite profile, by significantly promoting the production of taurine, tyrosol, and thymol, which act as potent activators of thermoregulation. Interestingly, the metabolic effects of naringin were abolished upon gut microbiota depletion through antibiotic intervention, concurrently leading the disappearance of naringin-induced thermogenesis and protective actions on diet-induced obesity. This discovery revealed a novel food-driven cross-sectional communication between gut bacteria and adipose tissues. Collectively, our data indicate that naringin supplementation stimulates BAT thermogenesis, alters fat distribution, promotes the browning process, and consequently inhibits body weight gain; importantly these metabolic effects require the participation of gut bacteria. |
| first_indexed | 2024-10-01T06:28:18Z |
| format | Journal Article |
| id | ntu-10356/175767 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:18Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1757672024-05-10T15:32:24Z Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice Li, Xiaoping Yao, Zhao Qi, Xinyue Cui, Jinling Zhou, Yuliang Tan, Yihong Huang, Xiaojun Ye, Hui School of Chemistry, Chemical Engineering and Biotechnology Medicine, Health and Life Sciences Naringin Obesity Naringin, a natural flavanone primarily found in citrus fruits, has garnered increased attention due to its recognized antioxidative, anti-inflammatory, and cardioprotective attributes. However, the functions of naringin in regulating energy expenditure are poorly understood. In the present study, we observed that twelve weeks of naringin supplementation substantially reshaped the metabolic profile of high-fat diet (HFD)-fed mice, by inhibiting body weight gain, reducing liver weight, and altering body compositions. Notably, naringin exhibited a remarkable capacity to augment whole-body energy expenditure of the tested mice by enhancing the thermogenic activity of brown adipose tissue (BAT) and stimulating browning of inguinal white adipose tissue (iWAT). Furthermore, our results showed naringin supplementation modified gut microbiota composition, specifically increasing the abundance of Bifidobacterium and Lachnospiraceae_bacterium_28-4, while reducing the abundance of Lachnospiraceae_bacterium_DW59 and Dubosiella_newyorkensis. Subsequently, we also found naringin supplementation altered fecal metabolite profile, by significantly promoting the production of taurine, tyrosol, and thymol, which act as potent activators of thermoregulation. Interestingly, the metabolic effects of naringin were abolished upon gut microbiota depletion through antibiotic intervention, concurrently leading the disappearance of naringin-induced thermogenesis and protective actions on diet-induced obesity. This discovery revealed a novel food-driven cross-sectional communication between gut bacteria and adipose tissues. Collectively, our data indicate that naringin supplementation stimulates BAT thermogenesis, alters fat distribution, promotes the browning process, and consequently inhibits body weight gain; importantly these metabolic effects require the participation of gut bacteria. Ministry of Education (MOE) Ministry of Health (MOH) Nanyang Technological University National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This work was supported by grants from National Research Foundation Singapore under its Young Individual Research Grant (023567- 00001) to HY and administered by Singapore Ministry of Health’s National Medical Research Council, Singapore MOE AcRF Tier 1 (RG90/ 23) to HY, NTU-Research Scholarship (R2301921) to XQ, Nature Science Foundation of Jiang Xi (General Program, 20224BAB205040) to XJ. 2024-05-06T06:00:52Z 2024-05-06T06:00:52Z 2024 Journal Article Li, X., Yao, Z., Qi, X., Cui, J., Zhou, Y., Tan, Y., Huang, X. & Ye, H. (2024). Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice. Current Research in Food Science, 8, 100683-. https://dx.doi.org/10.1016/j.crfs.2024.100683 2665-9271 https://hdl.handle.net/10356/175767 10.1016/j.crfs.2024.100683 38313225 2-s2.0-85183513135 8 100683 en 023567-00001 RG90/23 R2301921 Current Research in Food Science © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Naringin Obesity Li, Xiaoping Yao, Zhao Qi, Xinyue Cui, Jinling Zhou, Yuliang Tan, Yihong Huang, Xiaojun Ye, Hui Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title | Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title_full | Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title_fullStr | Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title_full_unstemmed | Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title_short | Naringin ameliorates obesity via stimulating adipose thermogenesis and browning, and modulating gut microbiota in diet-induced obese mice |
| title_sort | naringin ameliorates obesity via stimulating adipose thermogenesis and browning and modulating gut microbiota in diet induced obese mice |
| topic | Medicine, Health and Life Sciences Naringin Obesity |
| url | https://hdl.handle.net/10356/175767 |
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