Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance
This study investigates the diel cycle of airborne microorganisms indoors and its role as a proxy for indoor air quality. Using metagenomic analysis, 124 air samples were collected and analyzed on phylum, genus, and species level classifications. The study includes samples from two offices (Office 1...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/176039 |
| _version_ | 1811680138477174784 |
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| author | Luhung, Irvan Lim, Serene Boon Yuean Uchida, Akira Lim, Yee Hui Lau, Kenny J. X. Balakrishnan, Premkrishnan Schuster, Stephan Christoph |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Luhung, Irvan Lim, Serene Boon Yuean Uchida, Akira Lim, Yee Hui Lau, Kenny J. X. Balakrishnan, Premkrishnan Schuster, Stephan Christoph |
| author_sort | Luhung, Irvan |
| collection | NTU |
| description | This study investigates the diel cycle of airborne microorganisms indoors and its role as a proxy for indoor air quality. Using metagenomic analysis, 124 air samples were collected and analyzed on phylum, genus, and species level classifications. The study includes samples from two offices (Office 1 and Office 2) and an outdoor setting, each taken over six days and nights. Results show a clear day-night cycle for indoor bioaerosols in Office 1, distinct from the outdoor environment, with human-associated taxa dominating during the day. Office 2, considered polluted with mold, displays a divergent taxonomic profile lacking bacterial taxa. The study highlights the influence of mechanical ventilation on indoor bioaerosol dynamics, with night-time infiltration of outdoor air leading to the equilibration of indoor and outdoor air microbiomes. Furthermore, the study examines the effect of outdoor temperature on indoor air microbiome composition, observing disruptions in the outdoor diel cycle during a period of unusually low temperatures. Finally, the efficacy of mold removal and sanitation efforts is confirmed through follow-up air sampling, indicating significant reductions in Aspergillus versicolor and airborne fungal copy numbers. This comprehensive metagenomic survey sheds light on the role of indoor bioaerosol dynamics in instigating health related issues. Inclusion of such highly-resolved bioaerosol monitoring effort into the currently established air quality standards shall provide invaluable insights on improving indoor air quality through ventilation and sanitation efforts. |
| first_indexed | 2024-10-01T03:20:17Z |
| format | Journal Article |
| id | ntu-10356/176039 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:20:17Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1760392024-05-13T01:40:36Z Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance Luhung, Irvan Lim, Serene Boon Yuean Uchida, Akira Lim, Yee Hui Lau, Kenny J. X. Balakrishnan, Premkrishnan Schuster, Stephan Christoph Singapore Centre for Environmental Life Sciences and Engineering Engineering Bioaerosols Metagenomic surveillance This study investigates the diel cycle of airborne microorganisms indoors and its role as a proxy for indoor air quality. Using metagenomic analysis, 124 air samples were collected and analyzed on phylum, genus, and species level classifications. The study includes samples from two offices (Office 1 and Office 2) and an outdoor setting, each taken over six days and nights. Results show a clear day-night cycle for indoor bioaerosols in Office 1, distinct from the outdoor environment, with human-associated taxa dominating during the day. Office 2, considered polluted with mold, displays a divergent taxonomic profile lacking bacterial taxa. The study highlights the influence of mechanical ventilation on indoor bioaerosol dynamics, with night-time infiltration of outdoor air leading to the equilibration of indoor and outdoor air microbiomes. Furthermore, the study examines the effect of outdoor temperature on indoor air microbiome composition, observing disruptions in the outdoor diel cycle during a period of unusually low temperatures. Finally, the efficacy of mold removal and sanitation efforts is confirmed through follow-up air sampling, indicating significant reductions in Aspergillus versicolor and airborne fungal copy numbers. This comprehensive metagenomic survey sheds light on the role of indoor bioaerosol dynamics in instigating health related issues. Inclusion of such highly-resolved bioaerosol monitoring effort into the currently established air quality standards shall provide invaluable insights on improving indoor air quality through ventilation and sanitation efforts. Ministry of Education (MOE) This study was supported by Academic Research Fund Tier 3, Singapore Ministry of Education (Grant MOE2013-T3-1-013). The funder was not involved in study design, data collection, data analysis, writing of the study and decision on how the study will be published. 2024-05-13T01:40:36Z 2024-05-13T01:40:36Z 2024 Journal Article Luhung, I., Lim, S. B. Y., Uchida, A., Lim, Y. H., Lau, K. J. X., Balakrishnan, P. & Schuster, S. C. (2024). Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance. Building and Environment, 253, 111264-. https://dx.doi.org/10.1016/j.buildenv.2024.111264 0360-1323 https://hdl.handle.net/10356/176039 10.1016/j.buildenv.2024.111264 2-s2.0-85185196775 253 111264 en MOE2013-T3-1-013 Building and Environment © 2024 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering Bioaerosols Metagenomic surveillance Luhung, Irvan Lim, Serene Boon Yuean Uchida, Akira Lim, Yee Hui Lau, Kenny J. X. Balakrishnan, Premkrishnan Schuster, Stephan Christoph Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title | Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title_full | Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title_fullStr | Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title_full_unstemmed | Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title_short | Understanding diel bioaerosol patterns in mold-affected buildings through metagenomic surveillance |
| title_sort | understanding diel bioaerosol patterns in mold affected buildings through metagenomic surveillance |
| topic | Engineering Bioaerosols Metagenomic surveillance |
| url | https://hdl.handle.net/10356/176039 |
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