Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches
The rapid emergence of antibiotic resistance bacteria like Acinetobacter baumannii (A. baumannii) is becoming a global threat to public health calling for alternative therapeutic strategies. Consequently, there has been a rekindled interest in reviving phage therapy to prevent the misuse of antibiot...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2024
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| Online Access: | https://hdl.handle.net/10356/177197 |
| _version_ | 1826113829862375424 |
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| author | Palanivel Saravana Balaji |
| author2 | Duan Hongwei |
| author_facet | Duan Hongwei Palanivel Saravana Balaji |
| author_sort | Palanivel Saravana Balaji |
| collection | NTU |
| description | The rapid emergence of antibiotic resistance bacteria like Acinetobacter baumannii (A. baumannii) is becoming a global threat to public health calling for alternative therapeutic strategies. Consequently, there has been a rekindled interest in reviving phage therapy to prevent the misuse of antibiotics. Steering away from the traditional phage isolation methods, phages targeting A. baumannii were attempted to be discovered with targeted and non-targeted approaches from environmental metagenomics samples. While targeted approach focuses on
using conserve gene latching for phage characterisation with Polymerase Chain Reaction (PCR), the non-targeted Chromosome Conformation capture (Hi-C) approach employs proximity ligation to capture phage-host interactions in environmental samples. Despite the absence of A. baumannii phage from the Hi-C assembly reads, this methodology provides essential insights on the challenges faced in non-targeted phage discovery strategies highlighting the importance of optimising phage bioinformatics pipelines. In contrast, the targeted approach revealed the presence of a novel phage sequence discovered through the
application of Whole Genome Sequencing (WGS) providing a significant understanding the evolutionary dynamics existing between the phage and host. |
| first_indexed | 2024-10-01T03:29:19Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/177197 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:29:19Z |
| publishDate | 2024 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1771972024-05-31T15:32:10Z Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches Palanivel Saravana Balaji Duan Hongwei Yap Peng Huat, Eric School of Chemistry, Chemical Engineering and Biotechnology ericyap@ntu.edu.sg, hduan@ntu.edu.sg Medicine, Health and Life Sciences Bacteriophages Proximity ligation Antimicrobial resistance The rapid emergence of antibiotic resistance bacteria like Acinetobacter baumannii (A. baumannii) is becoming a global threat to public health calling for alternative therapeutic strategies. Consequently, there has been a rekindled interest in reviving phage therapy to prevent the misuse of antibiotics. Steering away from the traditional phage isolation methods, phages targeting A. baumannii were attempted to be discovered with targeted and non-targeted approaches from environmental metagenomics samples. While targeted approach focuses on using conserve gene latching for phage characterisation with Polymerase Chain Reaction (PCR), the non-targeted Chromosome Conformation capture (Hi-C) approach employs proximity ligation to capture phage-host interactions in environmental samples. Despite the absence of A. baumannii phage from the Hi-C assembly reads, this methodology provides essential insights on the challenges faced in non-targeted phage discovery strategies highlighting the importance of optimising phage bioinformatics pipelines. In contrast, the targeted approach revealed the presence of a novel phage sequence discovered through the application of Whole Genome Sequencing (WGS) providing a significant understanding the evolutionary dynamics existing between the phage and host. Bachelor's degree 2024-05-27T01:05:25Z 2024-05-27T01:05:25Z 2024 Final Year Project (FYP) Palanivel Saravana Balaji (2024). Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/177197 https://hdl.handle.net/10356/177197 en application/pdf Nanyang Technological University |
| spellingShingle | Medicine, Health and Life Sciences Bacteriophages Proximity ligation Antimicrobial resistance Palanivel Saravana Balaji Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title | Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title_full | Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title_fullStr | Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title_full_unstemmed | Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title_short | Discovery of bacteriophages in Acinetobacter baumannii: comparative analysis of targeted and non-targeted approaches |
| title_sort | discovery of bacteriophages in acinetobacter baumannii comparative analysis of targeted and non targeted approaches |
| topic | Medicine, Health and Life Sciences Bacteriophages Proximity ligation Antimicrobial resistance |
| url | https://hdl.handle.net/10356/177197 |
| work_keys_str_mv | AT palanivelsaravanabalaji discoveryofbacteriophagesinacinetobacterbaumanniicomparativeanalysisoftargetedandnontargetedapproaches |