Deciphering the phenotypes of mammary gland macrophage subsets during lactation
The mammary gland is a dynamic tissue that undergoes extensive remodeling in preparation for pregnancy and lactation. Its function is also affected by pregnancy complications such as gestational diabetes mellitus (GDM). However, it is unclear how these changes affect the composition and functions of...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2024
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| Online Access: | https://hdl.handle.net/10356/177315 |
| _version_ | 1811691886882062336 |
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| author | Tan, Yi Jie |
| author2 | Loh Jia Tong |
| author_facet | Loh Jia Tong Tan, Yi Jie |
| author_sort | Tan, Yi Jie |
| collection | NTU |
| description | The mammary gland is a dynamic tissue that undergoes extensive remodeling in preparation for pregnancy and lactation. Its function is also affected by pregnancy complications such as gestational diabetes mellitus (GDM). However, it is unclear how these changes affect the composition and functions of its cells. With the recent interest in the characterization of tissue-resident macrophages (TRMs) in the mammary gland, we conducted a comprehensive phenotypic analysis of macrophages (MØs) in the lactating mammary gland of mice at post-parturition day 7, 14, and 21 (P7, P14, P21) via high dimension flow cytometry and found ductal MØs as the dominant immune cell type in P14 mice. We also identified potential markers for improved characterization of different mammary MØ subsets, and observed elevated levels of ductal MØs in mammary glands of mice models induced with GDM versus healthy controls. This suggests the crucial role of ductal MØs in lactation as well as in diseased states like GDM. Taken together, this data provides further insight towards the knowledge of mammary TRM characterization and the discovery of potential novel TRM surface markers as therapeutic targets for GDM and beyond. |
| first_indexed | 2024-10-01T06:27:01Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/177315 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:01Z |
| publishDate | 2024 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1773152024-05-27T15:32:53Z Deciphering the phenotypes of mammary gland macrophage subsets during lactation Tan, Yi Jie Loh Jia Tong School of Biological Sciences AStar Singapore Immunology Network Chong Shu Zhen jiatong.loh@ntu.edu.sg, Chong_Shu_Zhen@immunol.a-star.edu.sg Medicine, Health and Life Sciences The mammary gland is a dynamic tissue that undergoes extensive remodeling in preparation for pregnancy and lactation. Its function is also affected by pregnancy complications such as gestational diabetes mellitus (GDM). However, it is unclear how these changes affect the composition and functions of its cells. With the recent interest in the characterization of tissue-resident macrophages (TRMs) in the mammary gland, we conducted a comprehensive phenotypic analysis of macrophages (MØs) in the lactating mammary gland of mice at post-parturition day 7, 14, and 21 (P7, P14, P21) via high dimension flow cytometry and found ductal MØs as the dominant immune cell type in P14 mice. We also identified potential markers for improved characterization of different mammary MØ subsets, and observed elevated levels of ductal MØs in mammary glands of mice models induced with GDM versus healthy controls. This suggests the crucial role of ductal MØs in lactation as well as in diseased states like GDM. Taken together, this data provides further insight towards the knowledge of mammary TRM characterization and the discovery of potential novel TRM surface markers as therapeutic targets for GDM and beyond. Bachelor's degree 2024-05-24T02:46:38Z 2024-05-24T02:46:38Z 2024 Final Year Project (FYP) Tan, Y. J. (2024). Deciphering the phenotypes of mammary gland macrophage subsets during lactation. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/177315 https://hdl.handle.net/10356/177315 en application/pdf Nanyang Technological University |
| spellingShingle | Medicine, Health and Life Sciences Tan, Yi Jie Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title | Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title_full | Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title_fullStr | Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title_full_unstemmed | Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title_short | Deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| title_sort | deciphering the phenotypes of mammary gland macrophage subsets during lactation |
| topic | Medicine, Health and Life Sciences |
| url | https://hdl.handle.net/10356/177315 |
| work_keys_str_mv | AT tanyijie decipheringthephenotypesofmammaryglandmacrophagesubsetsduringlactation |