Analysis of the plastic-degrading bacteria utilization
The environmentally friendly method of plastic degradation has always risen lately to be a topic of interest in society. This is due to the tendency of synthetic polymers to resist degradation in the natural environment and being left in the environment untreated in a continuously increasing volume....
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2024
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| Online Access: | https://hdl.handle.net/10356/177428 |
| _version_ | 1826119097899810816 |
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| author | Choi, Donghyun |
| author2 | Cao Bin |
| author_facet | Cao Bin Choi, Donghyun |
| author_sort | Choi, Donghyun |
| collection | NTU |
| description | The environmentally friendly method of plastic degradation has always risen lately to be a topic of interest in society. This is due to the tendency of synthetic polymers to resist degradation in the natural environment and being left in the environment untreated in a continuously increasing volume. Recently, in 2016, Japanese scientist, Kohei Oda, and his team identified a bacterium and named it Ideonella sakaiensis. The bacterium was initially separated from a mix of microorganisms in the sediment, comprising protozoa and yeast-like cells. It was demonstrated that the whole microbial population could convert 75% of the decomposed polyethylene terephthalate (PET) into carbon dioxide after it had been initially broken down and absorbed by Ideonella sakaiensis. This report will use Ideonella sakaiensis as a control and compare other bacteria growing on PET in a condition where nutrients are fed sufficiently. The comparison will be done through methods of DNA extraction and quantitative polymerase chain reaction (qPCR). From this comparison, bacteria that contain similarities with Ideonella sakaiensis could be obtained and possibly implemented as a PET degradation method. By using naturally growing bacteria, and environmentally friendly, plastic waste treatment methods, sustainability can be ensured. |
| first_indexed | 2024-10-01T04:54:30Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/177428 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:54:30Z |
| publishDate | 2024 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1774282024-05-31T15:35:25Z Analysis of the plastic-degrading bacteria utilization Choi, Donghyun Cao Bin School of Civil and Environmental Engineering BinCao@ntu.edu.sg Engineering The environmentally friendly method of plastic degradation has always risen lately to be a topic of interest in society. This is due to the tendency of synthetic polymers to resist degradation in the natural environment and being left in the environment untreated in a continuously increasing volume. Recently, in 2016, Japanese scientist, Kohei Oda, and his team identified a bacterium and named it Ideonella sakaiensis. The bacterium was initially separated from a mix of microorganisms in the sediment, comprising protozoa and yeast-like cells. It was demonstrated that the whole microbial population could convert 75% of the decomposed polyethylene terephthalate (PET) into carbon dioxide after it had been initially broken down and absorbed by Ideonella sakaiensis. This report will use Ideonella sakaiensis as a control and compare other bacteria growing on PET in a condition where nutrients are fed sufficiently. The comparison will be done through methods of DNA extraction and quantitative polymerase chain reaction (qPCR). From this comparison, bacteria that contain similarities with Ideonella sakaiensis could be obtained and possibly implemented as a PET degradation method. By using naturally growing bacteria, and environmentally friendly, plastic waste treatment methods, sustainability can be ensured. Bachelor's degree 2024-05-28T04:24:12Z 2024-05-28T04:24:12Z 2024 Final Year Project (FYP) Choi, D. (2024). Analysis of the plastic-degrading bacteria utilization. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/177428 https://hdl.handle.net/10356/177428 en application/pdf Nanyang Technological University |
| spellingShingle | Engineering Choi, Donghyun Analysis of the plastic-degrading bacteria utilization |
| title | Analysis of the plastic-degrading bacteria utilization |
| title_full | Analysis of the plastic-degrading bacteria utilization |
| title_fullStr | Analysis of the plastic-degrading bacteria utilization |
| title_full_unstemmed | Analysis of the plastic-degrading bacteria utilization |
| title_short | Analysis of the plastic-degrading bacteria utilization |
| title_sort | analysis of the plastic degrading bacteria utilization |
| topic | Engineering |
| url | https://hdl.handle.net/10356/177428 |
| work_keys_str_mv | AT choidonghyun analysisoftheplasticdegradingbacteriautilization |