Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations
To address the threat posed to agriculture due to the rise in global temperatures and other environmental stressors, we used Arabidopsis thaliana as a model organism to study the consequences of combined and consecutive exposure to varying temperatures and light intensities along fixed time interval...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2024
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| Online Access: | https://hdl.handle.net/10356/177558 |
| _version_ | 1811688992528138240 |
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| author | Manickavasagam, Nithya |
| author2 | Marek Mutwil |
| author_facet | Marek Mutwil Manickavasagam, Nithya |
| author_sort | Manickavasagam, Nithya |
| collection | NTU |
| description | To address the threat posed to agriculture due to the rise in global temperatures and other environmental stressors, we used Arabidopsis thaliana as a model organism to study the consequences of combined and consecutive exposure to varying temperatures and light intensities along fixed time intervals. Gene expression patterns of Heat shock protein 1, Heat shock factor A1, and Early-light-induced proteins 1 and 2 were studied to gain deeper insights into the transcriptomic response to stress exposure. Under the combined treatment regimen, high heat and high light inflicted the most damage on root phenotype and chlorophyll content, demonstrating the clear additive effect of heat and light stress. In contrast, consecutive application of heat followed by high light had an additive effect whereas high light followed by heat was similar to heat stress under medium light conditions. HsfA1 was also identified as a reliable biomarker for temperature stress, while, the interaction between high heat and light seems to have an antagonistic effect on the expression of ELIP1 and ELIP2. The results suggest that there are specific regulatory mechanisms governing the specific gene modules and physiological responses, which are influenced by the order in which the stresses are applied. |
| first_indexed | 2024-10-01T05:41:01Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/177558 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:41:01Z |
| publishDate | 2024 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1775582024-06-03T15:32:33Z Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations Manickavasagam, Nithya Marek Mutwil School of Biological Sciences Koh Youwei, Eugene mutwil@ntu.edu.sg Medicine, Health and Life Sciences To address the threat posed to agriculture due to the rise in global temperatures and other environmental stressors, we used Arabidopsis thaliana as a model organism to study the consequences of combined and consecutive exposure to varying temperatures and light intensities along fixed time intervals. Gene expression patterns of Heat shock protein 1, Heat shock factor A1, and Early-light-induced proteins 1 and 2 were studied to gain deeper insights into the transcriptomic response to stress exposure. Under the combined treatment regimen, high heat and high light inflicted the most damage on root phenotype and chlorophyll content, demonstrating the clear additive effect of heat and light stress. In contrast, consecutive application of heat followed by high light had an additive effect whereas high light followed by heat was similar to heat stress under medium light conditions. HsfA1 was also identified as a reliable biomarker for temperature stress, while, the interaction between high heat and light seems to have an antagonistic effect on the expression of ELIP1 and ELIP2. The results suggest that there are specific regulatory mechanisms governing the specific gene modules and physiological responses, which are influenced by the order in which the stresses are applied. Bachelor's degree 2024-05-29T04:35:01Z 2024-05-29T04:35:01Z 2024 Final Year Project (FYP) Manickavasagam, N. (2024). Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/177558 https://hdl.handle.net/10356/177558 en application/pdf Nanyang Technological University |
| spellingShingle | Medicine, Health and Life Sciences Manickavasagam, Nithya Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title | Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title_full | Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title_fullStr | Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title_full_unstemmed | Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title_short | Decoding the genetic memory of plants: investigating how past events shape their responses to stressful situations |
| title_sort | decoding the genetic memory of plants investigating how past events shape their responses to stressful situations |
| topic | Medicine, Health and Life Sciences |
| url | https://hdl.handle.net/10356/177558 |
| work_keys_str_mv | AT manickavasagamnithya decodingthegeneticmemoryofplantsinvestigatinghowpasteventsshapetheirresponsestostressfulsituations |