Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection
Evidence for a stable interaction between the respiratory syncytial virus (RSV) F and G proteins on the surface of virus filaments was provided using antibody immunoprecipitation studies on purified RSV particles, and by the in situ analysis on the surface of RSV-infected cells using the proximity l...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/177962 |
| _version_ | 1826130202854424576 |
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| author | Huong, Tra Nguyen Lee, Zhi Qi Lai, Soak Kuan Lee, Hsin Yee Tan, Boon Huan Sugrue, Richard J. |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Huong, Tra Nguyen Lee, Zhi Qi Lai, Soak Kuan Lee, Hsin Yee Tan, Boon Huan Sugrue, Richard J. |
| author_sort | Huong, Tra Nguyen |
| collection | NTU |
| description | Evidence for a stable interaction between the respiratory syncytial virus (RSV) F and G proteins on the surface of virus filaments was provided using antibody immunoprecipitation studies on purified RSV particles, and by the in situ analysis on the surface of RSV-infected cells using the proximity ligation assay. Imaging of the F and G protein distribution on virus filaments suggested that this protein complex was localised at the distal ends of the virus filaments, and suggested that this protein complex played a direct role in mediating efficient localised cell-to-cell virus transmission. G protein expression was required for efficient localised cell-to-cell transmission of RSV in cell monolayers which provided evidence that this protein complex mediates efficient multiple cycle infection. Collectively, these data provide evidence that F and G proteins form a complex on the surface of RSV particles, and that a role for this protein complex in promoting virus transmission is suggested. |
| first_indexed | 2024-10-01T07:52:39Z |
| format | Journal Article |
| id | ntu-10356/177962 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:52:39Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1779622024-06-03T07:42:31Z Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection Huong, Tra Nguyen Lee, Zhi Qi Lai, Soak Kuan Lee, Hsin Yee Tan, Boon Huan Sugrue, Richard J. Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences Medicine, Health and Life Sciences Respiratory syncytial virus Virus envelope Evidence for a stable interaction between the respiratory syncytial virus (RSV) F and G proteins on the surface of virus filaments was provided using antibody immunoprecipitation studies on purified RSV particles, and by the in situ analysis on the surface of RSV-infected cells using the proximity ligation assay. Imaging of the F and G protein distribution on virus filaments suggested that this protein complex was localised at the distal ends of the virus filaments, and suggested that this protein complex played a direct role in mediating efficient localised cell-to-cell virus transmission. G protein expression was required for efficient localised cell-to-cell transmission of RSV in cell monolayers which provided evidence that this protein complex mediates efficient multiple cycle infection. Collectively, these data provide evidence that F and G proteins form a complex on the surface of RSV particles, and that a role for this protein complex in promoting virus transmission is suggested. Ministry of Education (MOE) We thank the Ministry of Education, Republic of Singapore (RG59/12) for providing funding (awarded to RJS). 2024-06-03T07:42:31Z 2024-06-03T07:42:31Z 2024 Journal Article Huong, T. N., Lee, Z. Q., Lai, S. K., Lee, H. Y., Tan, B. H. & Sugrue, R. J. (2024). Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection. Virology, 591, 109985-. https://dx.doi.org/10.1016/j.virol.2024.109985 0042-6822 https://hdl.handle.net/10356/177962 10.1016/j.virol.2024.109985 38227992 2-s2.0-85183055128 591 109985 en RG59/12 Virology © 2024 Elsevier Inc. All rights reserved. |
| spellingShingle | Medicine, Health and Life Sciences Respiratory syncytial virus Virus envelope Huong, Tra Nguyen Lee, Zhi Qi Lai, Soak Kuan Lee, Hsin Yee Tan, Boon Huan Sugrue, Richard J. Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title | Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title_full | Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title_fullStr | Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title_full_unstemmed | Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title_short | Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| title_sort | evidence that an interaction between the respiratory syncytial virus f and g proteins at the distal ends of virus filaments mediates efficient multiple cycle infection |
| topic | Medicine, Health and Life Sciences Respiratory syncytial virus Virus envelope |
| url | https://hdl.handle.net/10356/177962 |
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