Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
The malaria parasite Plasmodium vivax remains a major global public health challenge, and no vaccine is approved for use in humans. Here, we assessed whether P. vivax strain-transcendent immunity can be achieved by repeated infection in Aotus monkeys. Sterile immunity was achieved after two homologo...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/178486 |
| _version_ | 1826126403837362176 |
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| author | Obaldía, Nicanor Da Silva Filho, Joao Luiz Núñez, Marlon Glass, Katherine A. Oulton, Tate Achcar, Fiona Wirjanata, Grennady Duraisingh, Manoj Felgner, Philip Tetteh, Kevin Ka Bozdech, Zbynek Otto, Thomas D. Marti, Matthias |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Obaldía, Nicanor Da Silva Filho, Joao Luiz Núñez, Marlon Glass, Katherine A. Oulton, Tate Achcar, Fiona Wirjanata, Grennady Duraisingh, Manoj Felgner, Philip Tetteh, Kevin Ka Bozdech, Zbynek Otto, Thomas D. Marti, Matthias |
| author_sort | Obaldía, Nicanor |
| collection | NTU |
| description | The malaria parasite Plasmodium vivax remains a major global public health challenge, and no vaccine is approved for use in humans. Here, we assessed whether P. vivax strain-transcendent immunity can be achieved by repeated infection in Aotus monkeys. Sterile immunity was achieved after two homologous infections, whereas subsequent heterologous challenge provided only partial protection. IgG levels based on P. vivax lysate ELISA and protein microarray increased with repeated infections and correlated with the level of homologous protection. Parasite transcriptional profiles provided no evidence of major antigenic switching upon homologous or heterologous challenge. However, we observed significant sequence diversity and transcriptional differences in the P. vivax core gene repertoire between the two strains used in the study, suggesting that partial protection upon heterologous challenge is due to molecular differences between strains rather than immune evasion by antigenic switching. Our study demonstrates that sterile immunity against P. vivax can be achieved by repeated homologous blood stage infection in Aotus monkeys, thus providing a benchmark to test the efficacy of candidate blood stage P. vivax malaria vaccines. |
| first_indexed | 2024-10-01T06:52:06Z |
| format | Journal Article |
| id | ntu-10356/178486 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:52:06Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1784862024-06-24T15:32:04Z Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model Obaldía, Nicanor Da Silva Filho, Joao Luiz Núñez, Marlon Glass, Katherine A. Oulton, Tate Achcar, Fiona Wirjanata, Grennady Duraisingh, Manoj Felgner, Philip Tetteh, Kevin Ka Bozdech, Zbynek Otto, Thomas D. Marti, Matthias School of Biological Sciences Medicine, Health and Life Sciences Aotidae Malaria The malaria parasite Plasmodium vivax remains a major global public health challenge, and no vaccine is approved for use in humans. Here, we assessed whether P. vivax strain-transcendent immunity can be achieved by repeated infection in Aotus monkeys. Sterile immunity was achieved after two homologous infections, whereas subsequent heterologous challenge provided only partial protection. IgG levels based on P. vivax lysate ELISA and protein microarray increased with repeated infections and correlated with the level of homologous protection. Parasite transcriptional profiles provided no evidence of major antigenic switching upon homologous or heterologous challenge. However, we observed significant sequence diversity and transcriptional differences in the P. vivax core gene repertoire between the two strains used in the study, suggesting that partial protection upon heterologous challenge is due to molecular differences between strains rather than immune evasion by antigenic switching. Our study demonstrates that sterile immunity against P. vivax can be achieved by repeated homologous blood stage infection in Aotus monkeys, thus providing a benchmark to test the efficacy of candidate blood stage P. vivax malaria vaccines. Published version Funding for this study was provided in part by core funds from the Gorgas Memorial Institute of Health Studies and the Sistema Nacional de Investigación de Panamá, SENACYT-SNI awarded to N Obaldía, Panamá, Panamá. This work was also supported by Royal Society Wolfson Research Merit Award (to M Marti) and Wellcome Trust Center Award 104111 (to F Achcar, JL Da Silva Filho, TD Otto, and M Marti). 2024-06-24T04:56:42Z 2024-06-24T04:56:42Z 2024 Journal Article Obaldía, N., Da Silva Filho, J. L., Núñez, M., Glass, K. A., Oulton, T., Achcar, F., Wirjanata, G., Duraisingh, M., Felgner, P., Tetteh, K. K., Bozdech, Z., Otto, T. D. & Marti, M. (2024). Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model. Life Science Alliance, 7(3), e202302524-. https://dx.doi.org/10.26508/lsa.202302524 2575-1077 https://hdl.handle.net/10356/178486 10.26508/lsa.202302524 38158220 2-s2.0-85181205207 3 7 e202302524 en Life Science Alliance © 2023 Obaldía et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Aotidae Malaria Obaldía, Nicanor Da Silva Filho, Joao Luiz Núñez, Marlon Glass, Katherine A. Oulton, Tate Achcar, Fiona Wirjanata, Grennady Duraisingh, Manoj Felgner, Philip Tetteh, Kevin Ka Bozdech, Zbynek Otto, Thomas D. Marti, Matthias Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title | Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title_full | Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title_fullStr | Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title_full_unstemmed | Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title_short | Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model |
| title_sort | sterile protection against p vivax malaria by repeated blood stage infection in the aotus monkey model |
| topic | Medicine, Health and Life Sciences Aotidae Malaria |
| url | https://hdl.handle.net/10356/178486 |
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