Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass
Chitin is a major component of various wastes such as crustacean shells, filamentous fungi, and insects. Recently, food-safe biological and chemical processes converting chitin to glucosamine have been developed. Here, we studied microalgae that can uptake glucosamine as vital carbon and nitrogen so...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/178598 |
| _version_ | 1811676756475641856 |
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| author | Elhalis, Hosam Helmy, Mohamed Ho, Sherilyn Leow, Sharon Liu, Yan Selvarajoo, Kumar Chow, Yvonne |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Elhalis, Hosam Helmy, Mohamed Ho, Sherilyn Leow, Sharon Liu, Yan Selvarajoo, Kumar Chow, Yvonne |
| author_sort | Elhalis, Hosam |
| collection | NTU |
| description | Chitin is a major component of various wastes such as crustacean shells, filamentous fungi, and insects. Recently, food-safe biological and chemical processes converting chitin to glucosamine have been developed. Here, we studied microalgae that can uptake glucosamine as vital carbon and nitrogen sources for valuable alternative protein biomass. Utilizing data mining and bioinformatics analysis, we identified 29 species that contain the required enzymes for glucosamine to glucose conversion. The growth performance of the selected strains was examined, and glucosamine was used in different forms and concentrations. Glucose at a concentration of 2.5 g/L was required to initiate glucosamine metabolic degradation by Chlorella vulgaris and Chlorella sorokiniana. Glucosamine HCl and glucosamine phosphate showed maximum cell counts of about 8.5 and 9.0 log/mL for C. sorokiniana and C. vulgaris in 14 days, respectively. Enzymatic hydrolysis of glucosamine increased growth performance with C. sorokiniana by about 3 folds. The adapted strains were fast-growing and could double their dry biomasses during the same incubation time. In addition, adapted C. sorokiniana was able to tolerate three times glucosamine concentration in the medium. The study illustrated possible strategies for employing C. sorokiniana and C. vulgaris to convert glucosamine into valuable biomass in a more sustainable way. |
| first_indexed | 2024-10-01T02:26:32Z |
| format | Journal Article |
| id | ntu-10356/178598 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:26:32Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1785982024-07-01T15:32:05Z Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass Elhalis, Hosam Helmy, Mohamed Ho, Sherilyn Leow, Sharon Liu, Yan Selvarajoo, Kumar Chow, Yvonne School of Biological Sciences Medicine, Health and Life Sciences Microalgae Glucosamine-6-phosphate deaminase Chitin is a major component of various wastes such as crustacean shells, filamentous fungi, and insects. Recently, food-safe biological and chemical processes converting chitin to glucosamine have been developed. Here, we studied microalgae that can uptake glucosamine as vital carbon and nitrogen sources for valuable alternative protein biomass. Utilizing data mining and bioinformatics analysis, we identified 29 species that contain the required enzymes for glucosamine to glucose conversion. The growth performance of the selected strains was examined, and glucosamine was used in different forms and concentrations. Glucose at a concentration of 2.5 g/L was required to initiate glucosamine metabolic degradation by Chlorella vulgaris and Chlorella sorokiniana. Glucosamine HCl and glucosamine phosphate showed maximum cell counts of about 8.5 and 9.0 log/mL for C. sorokiniana and C. vulgaris in 14 days, respectively. Enzymatic hydrolysis of glucosamine increased growth performance with C. sorokiniana by about 3 folds. The adapted strains were fast-growing and could double their dry biomasses during the same incubation time. In addition, adapted C. sorokiniana was able to tolerate three times glucosamine concentration in the medium. The study illustrated possible strategies for employing C. sorokiniana and C. vulgaris to convert glucosamine into valuable biomass in a more sustainable way. Agency for Science, Technology and Research (A*STAR) Published version This project was supported by the Agency for Science, Technology and Research under the Singapore Food Story R&D Programme (Theme 2 – 1st Alternative Protein Seed Challenge; W20W2D0017). Any opinions, findings, and conclusions, or recommendations expressed in this material are those of the authors and do not reflect the views of the Agency for Science, Technology and Research.The authors gratefully acknowledge financial support from the Agency for Science, Technology and Research (A*STAR). 2024-06-28T02:10:03Z 2024-06-28T02:10:03Z 2024 Journal Article Elhalis, H., Helmy, M., Ho, S., Leow, S., Liu, Y., Selvarajoo, K. & Chow, Y. (2024). Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass. Biotechnology Notes, 5, 13-22. https://dx.doi.org/10.1016/j.biotno.2024.01.003 2665-9069 https://hdl.handle.net/10356/178598 10.1016/j.biotno.2024.01.003 2-s2.0-85182800949 5 13 22 en W20W2D0017 Biotechnology Notes © 2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Microalgae Glucosamine-6-phosphate deaminase Elhalis, Hosam Helmy, Mohamed Ho, Sherilyn Leow, Sharon Liu, Yan Selvarajoo, Kumar Chow, Yvonne Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title | Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title_full | Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title_fullStr | Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title_full_unstemmed | Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title_short | Identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| title_sort | identifying chlorella vulgaris and chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass |
| topic | Medicine, Health and Life Sciences Microalgae Glucosamine-6-phosphate deaminase |
| url | https://hdl.handle.net/10356/178598 |
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