Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens
Background: While particular strains within the Bacillus species, such as Bacillus subtilis, have been commercially utilised as probiotics, it is critical to implement screening assays and evaluate the safety to identify potential Bacillus probiotic strains before clinical trials. This is because so...
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| Muut tekijät: | |
| Aineistotyyppi: | Journal Article |
| Kieli: | English |
| Julkaistu: |
2024
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| Aiheet: | |
| Linkit: | https://hdl.handle.net/10356/178812 |
| _version_ | 1826113615861645312 |
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| author | Zhao, Zhongtian Li, Wenrui Tran, The Thien Loo, Joachim Say Chye |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Zhao, Zhongtian Li, Wenrui Tran, The Thien Loo, Joachim Say Chye |
| author_sort | Zhao, Zhongtian |
| collection | NTU |
| description | Background: While particular strains within the Bacillus species, such as Bacillus subtilis, have been commercially utilised as probiotics, it is critical to implement screening assays and evaluate the safety to identify potential Bacillus probiotic strains before clinical trials. This is because some Bacillus species, including B. cereus and B. anthracis, can produce toxins that are harmful to humans. Results: In this study, we implemented a funnel-shaped approach to isolate and evaluate prospective probiotics from homogenised food waste – sesame oil meal (SOM). Of nine isolated strains with antipathogenic properties, B. subtilis SOM8 displayed the most promising activities against five listed human enteropathogens and was selected for further comprehensive assessment. B. subtilis SOM8 exhibited good tolerance when exposed to adverse stressors including acidity, bile salts, simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and heat treatment. Additionally, B. subtilis SOM8 possesses host-associated benefits such as antioxidant and bile salt hydrolase (BSH) activity. Furthermore, B. subtilis SOM8 contains only haemolysin toxin genes but has been proved to display partial haemolysis in the test and low cytotoxicity in Caco-2 cell models for in vitro evaluation. Moreover, B. subtilis SOM8 intrinsically resists only streptomycin and lacks plasmids or other mobile genetic elements. Bioinformatic analyses also predicted B. subtilis SOM8 encodes various bioactives compound like fengycin and lichendicin that could enable further biomedical applications. Conclusions: Our comprehensive evaluation revealed the substantial potential of B. subtilis SOM8 as a probiotic for targeting human enteropathogens, attributable to its exceptional performance across selection assays. Furthermore, our safety assessment, encompassing both phenotypic and genotypic analyses, showed B. subtilis SOM8 has a favourable preclinical safety profile, without significant threats to human health. Collectively, these findings highlight the promising prospects of B. subtilis SOM8 as a potent probiotic candidate for additional clinical development. |
| first_indexed | 2024-10-01T03:26:07Z |
| format | Journal Article |
| id | ntu-10356/178812 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:26:07Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1788122024-07-14T15:37:35Z Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens Zhao, Zhongtian Li, Wenrui Tran, The Thien Loo, Joachim Say Chye Lee Kong Chian School of Medicine (LKCMedicine) School of Materials Science and Engineering Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Medicine, Health and Life Sciences Probiotics Enteropathogen inhibition Background: While particular strains within the Bacillus species, such as Bacillus subtilis, have been commercially utilised as probiotics, it is critical to implement screening assays and evaluate the safety to identify potential Bacillus probiotic strains before clinical trials. This is because some Bacillus species, including B. cereus and B. anthracis, can produce toxins that are harmful to humans. Results: In this study, we implemented a funnel-shaped approach to isolate and evaluate prospective probiotics from homogenised food waste – sesame oil meal (SOM). Of nine isolated strains with antipathogenic properties, B. subtilis SOM8 displayed the most promising activities against five listed human enteropathogens and was selected for further comprehensive assessment. B. subtilis SOM8 exhibited good tolerance when exposed to adverse stressors including acidity, bile salts, simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and heat treatment. Additionally, B. subtilis SOM8 possesses host-associated benefits such as antioxidant and bile salt hydrolase (BSH) activity. Furthermore, B. subtilis SOM8 contains only haemolysin toxin genes but has been proved to display partial haemolysis in the test and low cytotoxicity in Caco-2 cell models for in vitro evaluation. Moreover, B. subtilis SOM8 intrinsically resists only streptomycin and lacks plasmids or other mobile genetic elements. Bioinformatic analyses also predicted B. subtilis SOM8 encodes various bioactives compound like fengycin and lichendicin that could enable further biomedical applications. Conclusions: Our comprehensive evaluation revealed the substantial potential of B. subtilis SOM8 as a probiotic for targeting human enteropathogens, attributable to its exceptional performance across selection assays. Furthermore, our safety assessment, encompassing both phenotypic and genotypic analyses, showed B. subtilis SOM8 has a favourable preclinical safety profile, without significant threats to human health. Collectively, these findings highlight the promising prospects of B. subtilis SOM8 as a potent probiotic candidate for additional clinical development. Ministry of Education (MOE) Singapore Food Agency Published version This Research Project was supported by the Singapore Food Agency (SFS_ RND_SUFP_001_06) and the Ministry of Education (RG79/22 and RT03/21). 2024-07-08T02:28:25Z 2024-07-08T02:28:25Z 2024 Journal Article Zhao, Z., Li, W., Tran, T. T. & Loo, J. S. C. (2024). Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens. BMC Microbiology, 24(1), 104-. https://dx.doi.org/10.1186/s12866-024-03263-y 1471-2180 https://hdl.handle.net/10356/178812 10.1186/s12866-024-03263-y 38539071 2-s2.0-85188904100 1 24 104 en SFS_RND_SUFP_001_06 RG79/22 RT03/21 BMC Microbiology © 2024 The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Probiotics Enteropathogen inhibition Zhao, Zhongtian Li, Wenrui Tran, The Thien Loo, Joachim Say Chye Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title | Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title_full | Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title_fullStr | Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title_full_unstemmed | Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title_short | Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| title_sort | bacillus subtilis som8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens |
| topic | Medicine, Health and Life Sciences Probiotics Enteropathogen inhibition |
| url | https://hdl.handle.net/10356/178812 |
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