Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites
We present the newly isolated Streptomyces sungeiensis SD3 strain as a promising microbial chassis for heterologous production of secondary metabolites. S. sungeiensis SD3 exhibits several advantageous traits as a microbial chassis, including genetic tractability, rapid growth, susceptibility to ant...
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/178869 |
| _version_ | 1811688133629050880 |
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| author | Lee, Sean Qiu En Ma, Guang-Lei Candra, Hartono Khandelwal, Srashti Pang, Li Mei Low, Zhen Jie Cheang, Qing Wei Liang, Zhao-Xun |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lee, Sean Qiu En Ma, Guang-Lei Candra, Hartono Khandelwal, Srashti Pang, Li Mei Low, Zhen Jie Cheang, Qing Wei Liang, Zhao-Xun |
| author_sort | Lee, Sean Qiu En |
| collection | NTU |
| description | We present the newly isolated Streptomyces sungeiensis SD3 strain as a promising microbial chassis for heterologous production of secondary metabolites. S. sungeiensis SD3 exhibits several advantageous traits as a microbial chassis, including genetic tractability, rapid growth, susceptibility to antibiotics, and metabolic capability supporting secondary metabolism. Genomic and transcriptomic sequencing unveiled the primary metabolic capabilities and secondary biosynthetic pathways of S. sungeiensis SD3, including a previously unknown pathway responsible for the biosynthesis of streptazone B1. The unique placement of S. sungeiensis SD3 in the phylogenetic tree designates it as a type strain, setting it apart from other frequently employed Streptomyces chassis. This distinction makes it the preferred chassis for expressing biosynthetic gene clusters (BGCs) derived from strains within the same phylogenetic or neighboring phylogenetic clade. The successful expression of secondary biosynthetic pathways from a closely related yet slow-growing strain underscores the utility of S. sungeiensis SD3 as a heterologous expression chassis. Validation of CRISPR/Cas9-assisted genetic tools for chromosomal deletion and insertion paved the way for further strain improvement and BGC refactoring through rational genome editing. The addition of S. sungeiensis SD3 to the heterologous chassis toolkit will facilitate the discovery and production of secondary metabolites. |
| first_indexed | 2024-10-01T05:27:22Z |
| format | Journal Article |
| id | ntu-10356/178869 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:27:22Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1788692024-07-15T15:32:28Z Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites Lee, Sean Qiu En Ma, Guang-Lei Candra, Hartono Khandelwal, Srashti Pang, Li Mei Low, Zhen Jie Cheang, Qing Wei Liang, Zhao-Xun School of Biological Sciences Medicine, Health and Life Sciences Streptomyces sungeiensis SD3 Microbial chassi We present the newly isolated Streptomyces sungeiensis SD3 strain as a promising microbial chassis for heterologous production of secondary metabolites. S. sungeiensis SD3 exhibits several advantageous traits as a microbial chassis, including genetic tractability, rapid growth, susceptibility to antibiotics, and metabolic capability supporting secondary metabolism. Genomic and transcriptomic sequencing unveiled the primary metabolic capabilities and secondary biosynthetic pathways of S. sungeiensis SD3, including a previously unknown pathway responsible for the biosynthesis of streptazone B1. The unique placement of S. sungeiensis SD3 in the phylogenetic tree designates it as a type strain, setting it apart from other frequently employed Streptomyces chassis. This distinction makes it the preferred chassis for expressing biosynthetic gene clusters (BGCs) derived from strains within the same phylogenetic or neighboring phylogenetic clade. The successful expression of secondary biosynthetic pathways from a closely related yet slow-growing strain underscores the utility of S. sungeiensis SD3 as a heterologous expression chassis. Validation of CRISPR/Cas9-assisted genetic tools for chromosomal deletion and insertion paved the way for further strain improvement and BGC refactoring through rational genome editing. The addition of S. sungeiensis SD3 to the heterologous chassis toolkit will facilitate the discovery and production of secondary metabolites. Ministry of Education (MOE) National Research Foundation (NRF) Submitted/Accepted version We are grateful for the generous financial support from NRF,Singapore (SBP-01 grant to Z.-X.L.) and MOE, Singapore(RG28/20 and MOE-T2EP30221-0010 to Z.-X.L.). 2024-07-10T05:28:53Z 2024-07-10T05:28:53Z 2024 Journal Article Lee, S. Q. E., Ma, G., Candra, H., Khandelwal, S., Pang, L. M., Low, Z. J., Cheang, Q. W. & Liang, Z. (2024). Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites. ACS Synthetic Biology, 13(4), 1259-1272. https://dx.doi.org/10.1021/acssynbio.3c00750 2161-5063 https://hdl.handle.net/10356/178869 10.1021/acssynbio.3c00750 38513222 2-s2.0-85188524859 4 13 1259 1272 en SBP-01 RG28/20 MOE-T2EP30221-0010 ACS Synthetic Biology 10.21979/N9/I4TNKA © 2024 American Chemical Society. All rights reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1021/acssynbio.3c00750. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Streptomyces sungeiensis SD3 Microbial chassi Lee, Sean Qiu En Ma, Guang-Lei Candra, Hartono Khandelwal, Srashti Pang, Li Mei Low, Zhen Jie Cheang, Qing Wei Liang, Zhao-Xun Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title | Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title_full | Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title_fullStr | Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title_full_unstemmed | Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title_short | Streptomyces sungeiensis SD3 as a microbial chassis for the heterologous production of secondary metabolites |
| title_sort | streptomyces sungeiensis sd3 as a microbial chassis for the heterologous production of secondary metabolites |
| topic | Medicine, Health and Life Sciences Streptomyces sungeiensis SD3 Microbial chassi |
| url | https://hdl.handle.net/10356/178869 |
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