Frozen enzyme EICP method for more effective soil improvement
Enzyme-induced calcite precipitation (EICP) is one of the emerging soil improvement methods. However, when plant-based enzyme is used, the urease enzyme harvested from plants cannot be stored long. This affects large-scale applications of this method. This paper presents a new method that not only e...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2024
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| Online Access: | https://hdl.handle.net/10356/179040 |
| _version_ | 1826112318787813376 |
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| author | Ng, Samuel Chu, Jian |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Ng, Samuel Chu, Jian |
| author_sort | Ng, Samuel |
| collection | NTU |
| description | Enzyme-induced calcite precipitation (EICP) is one of the emerging soil improvement methods. However, when plant-based enzyme is used, the urease enzyme harvested from plants cannot be stored long. This affects large-scale applications of this method. This paper presents a new method that not only enables urease enzyme to be stored for a long duration, but also improves significantly the effectiveness and efficiency of EICP for soil improvement. In this method, the storage duration of soybean derived urease enzyme is prolonged by storing it at negative 20 degrees. The experimental results indicated that the frozen-stored urease enzyme had an activity of 326% higher than that of fresh enzyme. The shear strength of a fine sand treated using the frozen-stored enzyme is 238.8% higher than that using a normal EICP method. Thus, the frozen method not only overcomes the enzyme storage problem, but also offers a much-improved EICP method. The reasons for the higher urease activity and improved strength enhancement are also explained in this paper. |
| first_indexed | 2024-10-01T03:05:03Z |
| format | Journal Article |
| id | ntu-10356/179040 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:03Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1790402024-07-19T15:33:31Z Frozen enzyme EICP method for more effective soil improvement Ng, Samuel Chu, Jian School of Civil and Environmental Engineering Earth and Environmental Sciences Biocement Soil improvement Enzyme-induced calcite precipitation (EICP) is one of the emerging soil improvement methods. However, when plant-based enzyme is used, the urease enzyme harvested from plants cannot be stored long. This affects large-scale applications of this method. This paper presents a new method that not only enables urease enzyme to be stored for a long duration, but also improves significantly the effectiveness and efficiency of EICP for soil improvement. In this method, the storage duration of soybean derived urease enzyme is prolonged by storing it at negative 20 degrees. The experimental results indicated that the frozen-stored urease enzyme had an activity of 326% higher than that of fresh enzyme. The shear strength of a fine sand treated using the frozen-stored enzyme is 238.8% higher than that using a normal EICP method. Thus, the frozen method not only overcomes the enzyme storage problem, but also offers a much-improved EICP method. The reasons for the higher urease activity and improved strength enhancement are also explained in this paper. Ministry of National Development (MND) Nanyang Technological University National Research Foundation (NRF) Submitted/Accepted version This research is supported by the Grant No. COT-V1-2020-4 provided by Ministry of National Development and National Research Foundation, Singapore under the Cities of Tomorrow R&D programme. The first author would also like to thank the financial support from NTU Research Scholarship awarded by Nanyang Technological University. 2024-07-16T08:37:45Z 2024-07-16T08:37:45Z 2024 Journal Article Ng, S. & Chu, J. (2024). Frozen enzyme EICP method for more effective soil improvement. Acta Geotechnica. https://dx.doi.org/10.1007/s11440-024-02348-2 1861-1125 https://hdl.handle.net/10356/179040 10.1007/s11440-024-02348-2 2-s2.0-85192994357 en COT-V1-2020-4 Acta Geotechnica © 2024 The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. All rights reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1007/s11440-024-02348-2. application/pdf |
| spellingShingle | Earth and Environmental Sciences Biocement Soil improvement Ng, Samuel Chu, Jian Frozen enzyme EICP method for more effective soil improvement |
| title | Frozen enzyme EICP method for more effective soil improvement |
| title_full | Frozen enzyme EICP method for more effective soil improvement |
| title_fullStr | Frozen enzyme EICP method for more effective soil improvement |
| title_full_unstemmed | Frozen enzyme EICP method for more effective soil improvement |
| title_short | Frozen enzyme EICP method for more effective soil improvement |
| title_sort | frozen enzyme eicp method for more effective soil improvement |
| topic | Earth and Environmental Sciences Biocement Soil improvement |
| url | https://hdl.handle.net/10356/179040 |
| work_keys_str_mv | AT ngsamuel frozenenzymeeicpmethodformoreeffectivesoilimprovement AT chujian frozenenzymeeicpmethodformoreeffectivesoilimprovement |