Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps
Vibrio parahaemolyticus is a severe pathogenic bacterium which causes significant mortality and economical loss in shrimp farming. To control vibriosis in whiteleg shrimps (Litopenaeus vannamei), we developed synbiotic beads by microencapsulating probiotic of Lactobacillus plantarum and prebiotic of...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/179099 |
| _version_ | 1811697560631377920 |
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| author | Wei, Guangmin Tay, Moon Yue Fen Kamaja, Vamshi Krishna Chan-Park, Mary B. |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Wei, Guangmin Tay, Moon Yue Fen Kamaja, Vamshi Krishna Chan-Park, Mary B. |
| author_sort | Wei, Guangmin |
| collection | NTU |
| description | Vibrio parahaemolyticus is a severe pathogenic bacterium which causes significant mortality and economical loss in shrimp farming. To control vibriosis in whiteleg shrimps (Litopenaeus vannamei), we developed synbiotic beads by microencapsulating probiotic of Lactobacillus plantarum and prebiotic of xanthan gum in agarose matrix through emulsion technique. Coculture method indicated that synbiotic beads reduce viability of Vibrio parahaemolyticus by 3 orders of magnitude. The high inhibitory activity of synbiotic beads against pathogen could result from the antibacterial substances secreted from encapsulated probiotics, and access blocking of pathogens to prebiotics as food source so that the encapsulated prebiotics solely stimulate nearby probiotic growth without boosting pathogen growth. Bacterial challenge test indicated that exposure to synbiotic beads in advance can delay mortality and increase shrimp survival from 10% to 57%. As a smart encapsulation platform, our design can be extended to various probiotics, prebiotics, and pathogens, thus providing an alternative strategy to the use of antibiotics for disease prevention and sustainable aquaculture. |
| first_indexed | 2024-10-01T07:57:12Z |
| format | Journal Article |
| id | ntu-10356/179099 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:57:12Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1790992024-07-17T08:04:51Z Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps Wei, Guangmin Tay, Moon Yue Fen Kamaja, Vamshi Krishna Chan-Park, Mary B. School of Chemistry, Chemical Engineering and Biotechnology NTU Food Technology Centre Centre for Antimicrobial Bioengineering Medicine, Health and Life Sciences Probiotic Prebiotic Vibrio parahaemolyticus is a severe pathogenic bacterium which causes significant mortality and economical loss in shrimp farming. To control vibriosis in whiteleg shrimps (Litopenaeus vannamei), we developed synbiotic beads by microencapsulating probiotic of Lactobacillus plantarum and prebiotic of xanthan gum in agarose matrix through emulsion technique. Coculture method indicated that synbiotic beads reduce viability of Vibrio parahaemolyticus by 3 orders of magnitude. The high inhibitory activity of synbiotic beads against pathogen could result from the antibacterial substances secreted from encapsulated probiotics, and access blocking of pathogens to prebiotics as food source so that the encapsulated prebiotics solely stimulate nearby probiotic growth without boosting pathogen growth. Bacterial challenge test indicated that exposure to synbiotic beads in advance can delay mortality and increase shrimp survival from 10% to 57%. As a smart encapsulation platform, our design can be extended to various probiotics, prebiotics, and pathogens, thus providing an alternative strategy to the use of antibiotics for disease prevention and sustainable aquaculture. Ministry of Education (MOE) This work was supported by the Singapore Ministry of Education (MOE) Tier 1 grant (RT08/20), MOE Tier 3 grant (MOE2018-T3–1-003), and NTU CARIE Funding. 2024-07-17T08:04:51Z 2024-07-17T08:04:51Z 2024 Journal Article Wei, G., Tay, M. Y. F., Kamaja, V. K. & Chan-Park, M. B. (2024). Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps. Aquaculture, 590, 741051-. https://dx.doi.org/10.1016/j.aquaculture.2024.741051 0044-8486 https://hdl.handle.net/10356/179099 10.1016/j.aquaculture.2024.741051 2-s2.0-85193588592 590 741051 en RT08/20 MOE2018-T3–1-003 Aquaculture © 2024 Published by Elsevier B.V. All rights reserved. |
| spellingShingle | Medicine, Health and Life Sciences Probiotic Prebiotic Wei, Guangmin Tay, Moon Yue Fen Kamaja, Vamshi Krishna Chan-Park, Mary B. Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title | Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title_full | Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title_fullStr | Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title_full_unstemmed | Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title_short | Synbiotic encapsulation against Vibrio parahaemolyticus infection in whiteleg shrimps |
| title_sort | synbiotic encapsulation against vibrio parahaemolyticus infection in whiteleg shrimps |
| topic | Medicine, Health and Life Sciences Probiotic Prebiotic |
| url | https://hdl.handle.net/10356/179099 |
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