Self-assembly of peptide nanocapsules by a solvent concentration gradient
Biological systems can create materials with intricate structures and specialized functions. In comparison, precise control of structures in human-made materials has been challenging. Here we report on insect cuticle peptides that spontaneously form nanocapsules through a single-step solvent exchang...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/179274 |
| _version_ | 1811682749002547200 |
|---|---|
| author | Li, Haopeng Qian, Xuliang Mohanram, Harini Han, Xiao Qi, Huitang Zou, Guijin Yuan, Fenghou Miserez, Ali Liu, Tian Yang, Qing Gao, Huajian Yu, Jing |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Li, Haopeng Qian, Xuliang Mohanram, Harini Han, Xiao Qi, Huitang Zou, Guijin Yuan, Fenghou Miserez, Ali Liu, Tian Yang, Qing Gao, Huajian Yu, Jing |
| author_sort | Li, Haopeng |
| collection | NTU |
| description | Biological systems can create materials with intricate structures and specialized functions. In comparison, precise control of structures in human-made materials has been challenging. Here we report on insect cuticle peptides that spontaneously form nanocapsules through a single-step solvent exchange process, where the concentration gradient resulting from the mixing of water and acetone drives the localization and self-assembly of the peptides into hollow nanocapsules. The underlying driving force is found to be the intrinsic affinity of the peptides for a particular solvent concentration, while the diffusion of water and acetone creates a gradient interface that triggers peptide localization and self-assembly. This gradient-mediated self-assembly offers a transformative pathway towards simple generation of drug delivery systems based on peptide nanocapsules. |
| first_indexed | 2024-10-01T04:01:47Z |
| format | Journal Article |
| id | ntu-10356/179274 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:01:47Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1792742024-07-24T05:56:49Z Self-assembly of peptide nanocapsules by a solvent concentration gradient Li, Haopeng Qian, Xuliang Mohanram, Harini Han, Xiao Qi, Huitang Zou, Guijin Yuan, Fenghou Miserez, Ali Liu, Tian Yang, Qing Gao, Huajian Yu, Jing School of Materials Science and Engineering School of Mechanical and Aerospace Engineering School of Biological Sciences Institute of High Performance Computing, A*STAR Biological & Biomimetic Material Laboratory @ NTU Center for Sustainable Materials Institute for Digital Molecular Analytics and Science Engineering Acetone Targeted drug delivery Biological systems can create materials with intricate structures and specialized functions. In comparison, precise control of structures in human-made materials has been challenging. Here we report on insect cuticle peptides that spontaneously form nanocapsules through a single-step solvent exchange process, where the concentration gradient resulting from the mixing of water and acetone drives the localization and self-assembly of the peptides into hollow nanocapsules. The underlying driving force is found to be the intrinsic affinity of the peptides for a particular solvent concentration, while the diffusion of water and acetone creates a gradient interface that triggers peptide localization and self-assembly. This gradient-mediated self-assembly offers a transformative pathway towards simple generation of drug delivery systems based on peptide nanocapsules. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) National Research Foundation (NRF) H.L., X.H. and J.Y acknowledge support from the Singapore National Research Fellowship (NRF-NRFF11-2019-0004) and the Singapore Ministry of Education (MOE) Tier 2 Grant (MOE-T2EP30220-0006). T.L. acknowledges support from the National Natural Science Foundation of China (31871959) and the National Key R&D Program of China (2022YFD1700200). Q.Y. acknowledges support from the National Natural Science Foundation of China (32161133010), the National Key R&D Program of China (2022YFD1700200) and the Shenzhen Science and Technology Program (KQTD20180411143628272). X.Q. and H.G. acknowledge support from the Singapore Ministry of Education (MOE) under its Academic Research Fund Tier 1 award no. RG138/20, no. RG135/22 and a start-up grant from Nanyang Technological University, Singapore and A*STAR, Singapore. G.Z and H.G. acknowledge funding support from the Ministry of Education in Singapore under grant MOE-MOET32022-0002. A.M. acknowledges support from the Singapore Ministry of Education (MOE) through an Academic Research (AcRF) Tier 3 grant (Grant No. MOE 2019-T3-1-012). We acknowledge the Facility for Analysis, Characterisation, Testing and Simulation (FACTS) and NTU Institute of Structural Biology (NISB), Nanyang Technological University, Singapore, for use of their HR-TEM, Cryo-TEM and NMR facilities. Molecular dynamics simulations reported were performed on resources provided by the High Performance Computing Centre at Nanyang Technological University, Singapore, and the National Supercomputing Centre, Singapore ( http://www.nscc.sg ). 2024-07-24T05:56:49Z 2024-07-24T05:56:49Z 2024 Journal Article Li, H., Qian, X., Mohanram, H., Han, X., Qi, H., Zou, G., Yuan, F., Miserez, A., Liu, T., Yang, Q., Gao, H. & Yu, J. (2024). Self-assembly of peptide nanocapsules by a solvent concentration gradient. Nature Nanotechnology. https://dx.doi.org/10.1038/s41565-024-01654-w 1748-3387 https://hdl.handle.net/10356/179274 10.1038/s41565-024-01654-w 38671050 2-s2.0-85191723843 en MOE-T2EP30220-0006 MOE 2019-T3-1-012 MOE-MOET32022-0002 NRFF11-2019-0004 RG138/20 RG135/22 Nature Nanotechnology © 2024 The Author(s), under exclusive licence to Springer Nature Limited. All rights reserved. |
| spellingShingle | Engineering Acetone Targeted drug delivery Li, Haopeng Qian, Xuliang Mohanram, Harini Han, Xiao Qi, Huitang Zou, Guijin Yuan, Fenghou Miserez, Ali Liu, Tian Yang, Qing Gao, Huajian Yu, Jing Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title | Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title_full | Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title_fullStr | Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title_full_unstemmed | Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title_short | Self-assembly of peptide nanocapsules by a solvent concentration gradient |
| title_sort | self assembly of peptide nanocapsules by a solvent concentration gradient |
| topic | Engineering Acetone Targeted drug delivery |
| url | https://hdl.handle.net/10356/179274 |
| work_keys_str_mv | AT lihaopeng selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT qianxuliang selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT mohanramharini selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT hanxiao selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT qihuitang selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT zouguijin selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT yuanfenghou selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT miserezali selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT liutian selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT yangqing selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT gaohuajian selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient AT yujing selfassemblyofpeptidenanocapsulesbyasolventconcentrationgradient |