Influence of laser pulse shape and cleanliness on two-photon microscopy
Nonlinear microscopy, including two-photon microscopy, requires pulsed lasers as light source. Typically, when choosing the appropriate pulsed laser for two-photon microscopy, the pulse repetition rate, pulse width, total power output, and output beam diameter are among the critical parameters which...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/179283 |
| _version_ | 1826125974651011072 |
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| author | Chong, Shau Poh Török, Peter |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Chong, Shau Poh Török, Peter |
| author_sort | Chong, Shau Poh |
| collection | NTU |
| description | Nonlinear microscopy, including two-photon microscopy, requires pulsed lasers as light source. Typically, when choosing the appropriate pulsed laser for two-photon microscopy, the pulse repetition rate, pulse width, total power output, and output beam diameter are among the critical parameters which are often emphasised. Here, we demonstrate that the pulse shape, often overlooked, can have significant impact on the two-photon microscopy excitation efficiency and the effective signal brightness. We provide metrics to ease practical selection of pulsed laser sources for two-photon microscopy. |
| first_indexed | 2024-10-01T06:45:09Z |
| format | Journal Article |
| id | ntu-10356/179283 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:45:09Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1792832024-07-25T15:30:24Z Influence of laser pulse shape and cleanliness on two-photon microscopy Chong, Shau Poh Török, Peter Lee Kong Chian School of Medicine (LKCMedicine) School of Physical and Mathematical Sciences Singapore Centre for Environmental Life Sciences and Engineering Institute for Digital Molecular Analytics and Science Engineering Pulse repetition rate Photons Nonlinear microscopy, including two-photon microscopy, requires pulsed lasers as light source. Typically, when choosing the appropriate pulsed laser for two-photon microscopy, the pulse repetition rate, pulse width, total power output, and output beam diameter are among the critical parameters which are often emphasised. Here, we demonstrate that the pulse shape, often overlooked, can have significant impact on the two-photon microscopy excitation efficiency and the effective signal brightness. We provide metrics to ease practical selection of pulsed laser sources for two-photon microscopy. Nanyang Technological University Published version 2024-07-25T01:18:12Z 2024-07-25T01:18:12Z 2024 Journal Article Chong, S. P. & Török, P. (2024). Influence of laser pulse shape and cleanliness on two-photon microscopy. Optics Continuum, 3(4), 552-564. https://dx.doi.org/10.1364/OPTCON.515123 2770-0208 https://hdl.handle.net/10356/179283 10.1364/OPTCON.515123 2-s2.0-85191742717 4 3 552 564 en Optics Continuum © 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement. application/pdf |
| spellingShingle | Engineering Pulse repetition rate Photons Chong, Shau Poh Török, Peter Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title | Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title_full | Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title_fullStr | Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title_full_unstemmed | Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title_short | Influence of laser pulse shape and cleanliness on two-photon microscopy |
| title_sort | influence of laser pulse shape and cleanliness on two photon microscopy |
| topic | Engineering Pulse repetition rate Photons |
| url | https://hdl.handle.net/10356/179283 |
| work_keys_str_mv | AT chongshaupoh influenceoflaserpulseshapeandcleanlinessontwophotonmicroscopy AT torokpeter influenceoflaserpulseshapeandcleanlinessontwophotonmicroscopy |