Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients
The aim of the present study was to investigate retinal microcirculatory and functional metabolic changes in patients after they had recovered from a moderate to severe acute COVID-19 infection. Retinal perfusion was quantified using laser speckle flowgraphy. Oxygen saturation and retinal calibers w...
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| Format: | Journal Article |
| Jezik: | English |
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2024
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| Online pristup: | https://hdl.handle.net/10356/179618 |
| _version_ | 1826115221934047232 |
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| author | Pai, Viktoria Bileck, Andrea Hommer, Nikolaus Janku, Patrick Lindner, Theresa Kauer, Victoria Rumpf, Benedikt Haslacher, Helmuth Hagn, Gerhard Meier-Menches, Samuel M. Schmetterer, Leopold Schmidl, Doreen Gerner, Christopher Garhöfer, Gerhard |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Pai, Viktoria Bileck, Andrea Hommer, Nikolaus Janku, Patrick Lindner, Theresa Kauer, Victoria Rumpf, Benedikt Haslacher, Helmuth Hagn, Gerhard Meier-Menches, Samuel M. Schmetterer, Leopold Schmidl, Doreen Gerner, Christopher Garhöfer, Gerhard |
| author_sort | Pai, Viktoria |
| collection | NTU |
| description | The aim of the present study was to investigate retinal microcirculatory and functional metabolic changes in patients after they had recovered from a moderate to severe acute COVID-19 infection. Retinal perfusion was quantified using laser speckle flowgraphy. Oxygen saturation and retinal calibers were assessed with a dynamic vessel analyzer. Arterio-venous ratio (AVR) was calculated based on retinal vessel diameter data. Blood plasma samples underwent mass spectrometry-based multi-omics profiling, including proteomics, metabolomics and eicosadomics. A total of 40 subjects were included in the present study, of which 29 had recovered from moderate to severe COVID-19 within 2 to 23 weeks before inclusion and 11 had never had COVID-19, as confirmed by antibody testing. Perfusion in retinal vessels was significantly lower in patients (60.6 ± 16.0 a.u.) than in control subjects (76.2 ± 12.1 a.u., p = 0.006). Arterio-venous (AV) difference in oxygen saturation and AVR was significantly lower in patients compared to healthy controls (p = 0.021 for AVR and p = 0.023 for AV difference in oxygen saturation). Molecular profiles demonstrated down-regulation of cell adhesion molecules, NOTCH3 and fatty acids, and suggested a bisphasic dysregulation of nitric oxide synthesis after COVID-19 infection. The results of this study imply that retinal perfusion and oxygen metabolism is still significantly altered in patients well beyond the acute phase of COVID-19. This is also reflected in the molecular profiling analysis of blood plasma, indicating a down-regulation of nitric oxide-related endothelial and immunological cell functions. Trial Registration: ClinicalTrials.gov (https://clinicaltrials.gov) NCT05650905. |
| first_indexed | 2024-10-01T03:52:00Z |
| format | Journal Article |
| id | ntu-10356/179618 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:52:00Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1796182024-08-16T15:31:58Z Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients Pai, Viktoria Bileck, Andrea Hommer, Nikolaus Janku, Patrick Lindner, Theresa Kauer, Victoria Rumpf, Benedikt Haslacher, Helmuth Hagn, Gerhard Meier-Menches, Samuel M. Schmetterer, Leopold Schmidl, Doreen Gerner, Christopher Garhöfer, Gerhard School of Chemistry, Chemical Engineering and Biotechnology Singapore National Eye Centre Duke-NUS Medical School SERI-NTU Advanced Ocular Engineering (STANCE), Singapore Medicine, Health and Life Sciences Ocular blood flow Metabolomics The aim of the present study was to investigate retinal microcirculatory and functional metabolic changes in patients after they had recovered from a moderate to severe acute COVID-19 infection. Retinal perfusion was quantified using laser speckle flowgraphy. Oxygen saturation and retinal calibers were assessed with a dynamic vessel analyzer. Arterio-venous ratio (AVR) was calculated based on retinal vessel diameter data. Blood plasma samples underwent mass spectrometry-based multi-omics profiling, including proteomics, metabolomics and eicosadomics. A total of 40 subjects were included in the present study, of which 29 had recovered from moderate to severe COVID-19 within 2 to 23 weeks before inclusion and 11 had never had COVID-19, as confirmed by antibody testing. Perfusion in retinal vessels was significantly lower in patients (60.6 ± 16.0 a.u.) than in control subjects (76.2 ± 12.1 a.u., p = 0.006). Arterio-venous (AV) difference in oxygen saturation and AVR was significantly lower in patients compared to healthy controls (p = 0.021 for AVR and p = 0.023 for AV difference in oxygen saturation). Molecular profiles demonstrated down-regulation of cell adhesion molecules, NOTCH3 and fatty acids, and suggested a bisphasic dysregulation of nitric oxide synthesis after COVID-19 infection. The results of this study imply that retinal perfusion and oxygen metabolism is still significantly altered in patients well beyond the acute phase of COVID-19. This is also reflected in the molecular profiling analysis of blood plasma, indicating a down-regulation of nitric oxide-related endothelial and immunological cell functions. Trial Registration: ClinicalTrials.gov (https://clinicaltrials.gov) NCT05650905. Published version This research was funded in whole or in part by the Austrian Science Fund (FWF) [KLI721]. 2024-08-13T05:30:35Z 2024-08-13T05:30:35Z 2024 Journal Article Pai, V., Bileck, A., Hommer, N., Janku, P., Lindner, T., Kauer, V., Rumpf, B., Haslacher, H., Hagn, G., Meier-Menches, S. M., Schmetterer, L., Schmidl, D., Gerner, C. & Garhöfer, G. (2024). Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients. Scientific Reports, 14(1). https://dx.doi.org/10.1038/s41598-024-56834-4 2045-2322 https://hdl.handle.net/10356/179618 10.1038/s41598-024-56834-4 14 2-s2.0-85189981682 1 14 en Scientific Reports © 2024 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Ocular blood flow Metabolomics Pai, Viktoria Bileck, Andrea Hommer, Nikolaus Janku, Patrick Lindner, Theresa Kauer, Victoria Rumpf, Benedikt Haslacher, Helmuth Hagn, Gerhard Meier-Menches, Samuel M. Schmetterer, Leopold Schmidl, Doreen Gerner, Christopher Garhöfer, Gerhard Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title | Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title_full | Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title_fullStr | Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title_full_unstemmed | Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title_short | Impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered COVID-19 patients |
| title_sort | impaired retinal oxygen metabolism and perfusion are accompanied by plasma protein and lipid alterations in recovered covid 19 patients |
| topic | Medicine, Health and Life Sciences Ocular blood flow Metabolomics |
| url | https://hdl.handle.net/10356/179618 |
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