Blood microbial signatures associated with mortality in patients with sepsis: a pilot study
Sepsis is a life-threatening illness caused by the dysregulated host response to infection. Nevertheless, our current knowledge of the microbial landscape in the blood of septic patients is still limited. Next-generation sequencing (NGS) is a sensitive method to quantitatively characterize microbiom...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10356/179713 |
| _version_ | 1826122020139565056 |
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| author | Chen, Huarong Liu, Weixin Coker, Olabisi Oluwabukola Qin, Na Chen, Hongyan Wang, Yifei Liu, Xiaodong Zhang, Lin Choi, Gordon Y. S. Wong, Wai Tat Leung, Czarina C. H. Ling, Lowell Hui, Mamie Gin, Tony Wong, Sunny Hei Chan, Matthew Tak Vai Wu, William Ka Kei |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Chen, Huarong Liu, Weixin Coker, Olabisi Oluwabukola Qin, Na Chen, Hongyan Wang, Yifei Liu, Xiaodong Zhang, Lin Choi, Gordon Y. S. Wong, Wai Tat Leung, Czarina C. H. Ling, Lowell Hui, Mamie Gin, Tony Wong, Sunny Hei Chan, Matthew Tak Vai Wu, William Ka Kei |
| author_sort | Chen, Huarong |
| collection | NTU |
| description | Sepsis is a life-threatening illness caused by the dysregulated host response to infection. Nevertheless, our current knowledge of the microbial landscape in the blood of septic patients is still limited. Next-generation sequencing (NGS) is a sensitive method to quantitatively characterize microbiomes at various sites of the human body. In this study, we analyzed the blood microbial DNA of 22 adult patients with sepsis and 3 healthy subjects. The presence of non-human DNA was identified in both healthy and septic subjects. Septic patients had a markedly altered microbial DNA profile compared to healthy subjects over α- and β-diversity. Unexpectedly, the patients could be further divided into two subgroups (C1 and C2) based on β-diversity analysis. C1 patients showed much higher bacteria, viruses, fungi, and archaea abundance, and a higher level of α-diversity (Chao1, Observed and Shannon index) than both C2 patients and healthy subjects. The most striking difference was seen in the case of Streptomyces violaceusniger, Phenylobacterium sp. HYN0004, Caulobacter flavus, Streptomyces sp. 11-1-2, and Phenylobacterium zucineum, the abundance of which was the highest in the C1 group. Notably, C1 patients had a significantly poorer outcome than C2 patients. Moreover, by analyzing the patterns of microbe-microbe interactions in healthy and septic subjects, we revealed that C1 and C2 patients exhibited distinct co-occurrence and co-exclusion relationships. Together, our study uncovered two distinct microbial signatures in the blood of septic patients. Compositional and ecological analysis of blood microbial DNA may thus be useful in predicting mortality of septic patients. |
| first_indexed | 2024-10-01T05:41:40Z |
| format | Journal Article |
| id | ntu-10356/179713 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:41:40Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1797132024-08-25T15:38:09Z Blood microbial signatures associated with mortality in patients with sepsis: a pilot study Chen, Huarong Liu, Weixin Coker, Olabisi Oluwabukola Qin, Na Chen, Hongyan Wang, Yifei Liu, Xiaodong Zhang, Lin Choi, Gordon Y. S. Wong, Wai Tat Leung, Czarina C. H. Ling, Lowell Hui, Mamie Gin, Tony Wong, Sunny Hei Chan, Matthew Tak Vai Wu, William Ka Kei Lee Kong Chian School of Medicine (LKCMedicine) Tan Tock Seng Hospital Medicine, Health and Life Sciences Sepsis Microorganism Sepsis is a life-threatening illness caused by the dysregulated host response to infection. Nevertheless, our current knowledge of the microbial landscape in the blood of septic patients is still limited. Next-generation sequencing (NGS) is a sensitive method to quantitatively characterize microbiomes at various sites of the human body. In this study, we analyzed the blood microbial DNA of 22 adult patients with sepsis and 3 healthy subjects. The presence of non-human DNA was identified in both healthy and septic subjects. Septic patients had a markedly altered microbial DNA profile compared to healthy subjects over α- and β-diversity. Unexpectedly, the patients could be further divided into two subgroups (C1 and C2) based on β-diversity analysis. C1 patients showed much higher bacteria, viruses, fungi, and archaea abundance, and a higher level of α-diversity (Chao1, Observed and Shannon index) than both C2 patients and healthy subjects. The most striking difference was seen in the case of Streptomyces violaceusniger, Phenylobacterium sp. HYN0004, Caulobacter flavus, Streptomyces sp. 11-1-2, and Phenylobacterium zucineum, the abundance of which was the highest in the C1 group. Notably, C1 patients had a significantly poorer outcome than C2 patients. Moreover, by analyzing the patterns of microbe-microbe interactions in healthy and septic subjects, we revealed that C1 and C2 patients exhibited distinct co-occurrence and co-exclusion relationships. Together, our study uncovered two distinct microbial signatures in the blood of septic patients. Compositional and ecological analysis of blood microbial DNA may thus be useful in predicting mortality of septic patients. Published version This project was supported by Commissioned Research on Control of Infectious Diseases (Phase III) (CU-15-B2) of the Food and Health Bureau of Hong Kong; Heath and Medical Research Fund (HMRF) (22210032); RGC-GRF Hong Kong (14107321, 14101922); the National Natural Science Foundation of China (NSFC) (82272989); and CUHK Direct Grant for Research (2022.003).This project was supported by Commissioned Research on Control of Infectious Diseases (Phase III) (CU-15-B2) of the Food and Health Bureau of Hong Kong; Heath and Medical Research Fund (HMRF) (22210032); RGC-GRF Hong Kong (14107321, 14101922); the National Natural Science Foundation of China (NSFC) (82272989); and CUHK Direct Grant for Research (2022.003). 2024-08-19T06:39:16Z 2024-08-19T06:39:16Z 2024 Journal Article Chen, H., Liu, W., Coker, O. O., Qin, N., Chen, H., Wang, Y., Liu, X., Zhang, L., Choi, G. Y. S., Wong, W. T., Leung, C. C. H., Ling, L., Hui, M., Gin, T., Wong, S. H., Chan, M. T. V. & Wu, W. K. K. (2024). Blood microbial signatures associated with mortality in patients with sepsis: a pilot study. Heliyon, 10(8), e29572-. https://dx.doi.org/10.1016/j.heliyon.2024.e29572 2405-8440 https://hdl.handle.net/10356/179713 10.1016/j.heliyon.2024.e29572 38699748 2-s2.0-85190776829 8 10 e29572 en Heliyon © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/). application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Sepsis Microorganism Chen, Huarong Liu, Weixin Coker, Olabisi Oluwabukola Qin, Na Chen, Hongyan Wang, Yifei Liu, Xiaodong Zhang, Lin Choi, Gordon Y. S. Wong, Wai Tat Leung, Czarina C. H. Ling, Lowell Hui, Mamie Gin, Tony Wong, Sunny Hei Chan, Matthew Tak Vai Wu, William Ka Kei Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title | Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title_full | Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title_fullStr | Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title_full_unstemmed | Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title_short | Blood microbial signatures associated with mortality in patients with sepsis: a pilot study |
| title_sort | blood microbial signatures associated with mortality in patients with sepsis a pilot study |
| topic | Medicine, Health and Life Sciences Sepsis Microorganism |
| url | https://hdl.handle.net/10356/179713 |
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