Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation"
Automated air-displacement pipettes have become a standard equipment for the transfer of liquids in laboratory settings. However, these tools fail to perform accurate and precise transfers of liquids with viscosities greater than 100 cP. In this study, we report a systematic protocol for optimizing...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/179819 |
| _version_ | 1811691986361516032 |
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| author | Velasco, Pablo Quijano Low, Andre Kai Yuan Leong, Chang Jie Ng, Wan Ting Qiu, Selina Jhunjhunwala, Shivam Li, Bryant Qian, Anne Hippalgaonkar, Kedar Cheng, Jayce Jian Wei |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Velasco, Pablo Quijano Low, Andre Kai Yuan Leong, Chang Jie Ng, Wan Ting Qiu, Selina Jhunjhunwala, Shivam Li, Bryant Qian, Anne Hippalgaonkar, Kedar Cheng, Jayce Jian Wei |
| author_sort | Velasco, Pablo Quijano |
| collection | NTU |
| description | Automated air-displacement pipettes have become a standard equipment for the transfer of liquids in laboratory settings. However, these tools fail to perform accurate and precise transfers of liquids with viscosities greater than 100 cP. In this study, we report a systematic protocol for optimizing the liquid-handling parameters of automated pipettes to achieve accurate and precise transfers of viscous liquids (within 5% of percentage transfer error) with viscosities as high as 1275 cP using minimal transfer times. The protocol is based on the iterative gravimetric testing of different combinations of aspiration and dispense rates obtained by a Multi-Objective Bayesian Optimization (MOBO) algorithm. We demonstrate that optimal solutions obtained through MOBO can match or outperform solutions derived from human intuition, showing a consistent performance even when different pipetting equipment and tip geometries are used. Finally, we demonstrate that the protocol can be performed in a fully automated, closed-loop fashion by integrating an automated mass balance, increasing the manpower efficiency of the method. This offers a valuable advancement in the accurate manipulation of highly viscous liquids, with broad applications for the automation of various laboratory experiments. |
| first_indexed | 2024-10-01T06:28:36Z |
| format | Journal Article |
| id | ntu-10356/179819 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:36Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1798192024-08-30T15:45:25Z Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" Velasco, Pablo Quijano Low, Andre Kai Yuan Leong, Chang Jie Ng, Wan Ting Qiu, Selina Jhunjhunwala, Shivam Li, Bryant Qian, Anne Hippalgaonkar, Kedar Cheng, Jayce Jian Wei School of Materials Science and Engineering Institute of Materials Research and Engineering, A*STAR Engineering Pipettes Viscous liquid transfers Automated air-displacement pipettes have become a standard equipment for the transfer of liquids in laboratory settings. However, these tools fail to perform accurate and precise transfers of liquids with viscosities greater than 100 cP. In this study, we report a systematic protocol for optimizing the liquid-handling parameters of automated pipettes to achieve accurate and precise transfers of viscous liquids (within 5% of percentage transfer error) with viscosities as high as 1275 cP using minimal transfer times. The protocol is based on the iterative gravimetric testing of different combinations of aspiration and dispense rates obtained by a Multi-Objective Bayesian Optimization (MOBO) algorithm. We demonstrate that optimal solutions obtained through MOBO can match or outperform solutions derived from human intuition, showing a consistent performance even when different pipetting equipment and tip geometries are used. Finally, we demonstrate that the protocol can be performed in a fully automated, closed-loop fashion by integrating an automated mass balance, increasing the manpower efficiency of the method. This offers a valuable advancement in the accurate manipulation of highly viscous liquids, with broad applications for the automation of various laboratory experiments. Agency for Science, Technology and Research (A*STAR) Published version This work was supported by A*STAR under an MTC AME Programmatic Grant (Grant number A20H3b0140) for Structural Power for portable and electrified transportation. 2024-08-26T07:09:36Z 2024-08-26T07:09:36Z 2024 Journal Article Velasco, P. Q., Low, A. K. Y., Leong, C. J., Ng, W. T., Qiu, S., Jhunjhunwala, S., Li, B., Qian, A., Hippalgaonkar, K. & Cheng, J. J. W. (2024). Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation". Digital Discovery, 3(5), 1011-1020. https://dx.doi.org/10.1039/d3dd00255a 2635-098X https://hdl.handle.net/10356/179819 10.1039/d3dd00255a 2-s2.0-85191311592 5 3 1011 1020 en A20H3b0140 Digital Discovery © 2024 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. application/pdf |
| spellingShingle | Engineering Pipettes Viscous liquid transfers Velasco, Pablo Quijano Low, Andre Kai Yuan Leong, Chang Jie Ng, Wan Ting Qiu, Selina Jhunjhunwala, Shivam Li, Bryant Qian, Anne Hippalgaonkar, Kedar Cheng, Jayce Jian Wei Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title | Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title_full | Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title_fullStr | Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title_full_unstemmed | Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title_short | Optimization of liquid handling parameters for viscous liquid transfers with pipetting robots, a "sticky situation" |
| title_sort | optimization of liquid handling parameters for viscous liquid transfers with pipetting robots a sticky situation |
| topic | Engineering Pipettes Viscous liquid transfers |
| url | https://hdl.handle.net/10356/179819 |
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