Bacterial attachment by crystal in MICP
Microbially induced calcium carbonate precipitation (MICP) is recognized as a promising technique for soil improvement. The morphological evolution of calcium carbonate (CaCO3) crystals during the MICP process significantly impacts the engineering properties of biocemented soils. However, the morpho...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/179904 |
| _version_ | 1826110327857610752 |
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| author | Liu, Hanlong Zhang, Jinxuan Xiao, Yang He, Xiang |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Liu, Hanlong Zhang, Jinxuan Xiao, Yang He, Xiang |
| author_sort | Liu, Hanlong |
| collection | NTU |
| description | Microbially induced calcium carbonate precipitation (MICP) is recognized as a promising technique for soil improvement. The morphological evolution of calcium carbonate (CaCO3) crystals during the MICP process significantly impacts the engineering properties of biocemented soils. However, the morphological changes of CaCO3 precipitates upon bacterial adsorption onto crystal surfaces have not been sufficiently studied. This study employs real-time laser scanning confocal microscopy (LSCM) to simultaneously monitor the dynamics of CaCO3 growth and bacterial attachment during the MICP process, while fluorescence staining is used to differentiate between living and dead bacteria. The results indicate that during the initial stage of the MICP process, the predominant morphology of the CaCO3 crystals was elliptical, with a minor fraction exhibiting a rhombohedral morphology. Over time, additional elliptical crystals gradually formed around the existing elliptical ones. As the crystals grew, certain bacteria in the vicinity of the crystals became adsorbed onto their surfaces, irrespective of bacterial viability. However, bacterial adsorption did not alter the morphology of the crystals. The study provides microscale insights into the mechanisms of bacterial attachment to CaCO3 crystals during biomineralization. |
| first_indexed | 2024-10-01T02:32:36Z |
| format | Journal Article |
| id | ntu-10356/179904 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:32:36Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1799042024-09-06T15:33:28Z Bacterial attachment by crystal in MICP Liu, Hanlong Zhang, Jinxuan Xiao, Yang He, Xiang School of Civil and Environmental Engineering Engineering Confocal microscopy Mineral morphology Microbially induced calcium carbonate precipitation (MICP) is recognized as a promising technique for soil improvement. The morphological evolution of calcium carbonate (CaCO3) crystals during the MICP process significantly impacts the engineering properties of biocemented soils. However, the morphological changes of CaCO3 precipitates upon bacterial adsorption onto crystal surfaces have not been sufficiently studied. This study employs real-time laser scanning confocal microscopy (LSCM) to simultaneously monitor the dynamics of CaCO3 growth and bacterial attachment during the MICP process, while fluorescence staining is used to differentiate between living and dead bacteria. The results indicate that during the initial stage of the MICP process, the predominant morphology of the CaCO3 crystals was elliptical, with a minor fraction exhibiting a rhombohedral morphology. Over time, additional elliptical crystals gradually formed around the existing elliptical ones. As the crystals grew, certain bacteria in the vicinity of the crystals became adsorbed onto their surfaces, irrespective of bacterial viability. However, bacterial adsorption did not alter the morphology of the crystals. The study provides microscale insights into the mechanisms of bacterial attachment to CaCO3 crystals during biomineralization. Published version 2024-09-02T05:06:55Z 2024-09-02T05:06:55Z 2024 Journal Article Liu, H., Zhang, J., Xiao, Y. & He, X. (2024). Bacterial attachment by crystal in MICP. Biogeotechnics, 2(4), 100109-. https://dx.doi.org/10.1016/j.bgtech.2024.100109 2949-9291 https://hdl.handle.net/10356/179904 10.1016/j.bgtech.2024.100109 2-s2.0-85195053544 4 2 100109 en Biogeotechnics © 2024 The Author(s). Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
| spellingShingle | Engineering Confocal microscopy Mineral morphology Liu, Hanlong Zhang, Jinxuan Xiao, Yang He, Xiang Bacterial attachment by crystal in MICP |
| title | Bacterial attachment by crystal in MICP |
| title_full | Bacterial attachment by crystal in MICP |
| title_fullStr | Bacterial attachment by crystal in MICP |
| title_full_unstemmed | Bacterial attachment by crystal in MICP |
| title_short | Bacterial attachment by crystal in MICP |
| title_sort | bacterial attachment by crystal in micp |
| topic | Engineering Confocal microscopy Mineral morphology |
| url | https://hdl.handle.net/10356/179904 |
| work_keys_str_mv | AT liuhanlong bacterialattachmentbycrystalinmicp AT zhangjinxuan bacterialattachmentbycrystalinmicp AT xiaoyang bacterialattachmentbycrystalinmicp AT hexiang bacterialattachmentbycrystalinmicp |