Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy
Purpose: To assess intra- (repeatability) and inter-observer (reproducibility) variability of laser speckle flowgraphy (LSFG) for retinal blood flow (RBF) measurement in 20 eyes of wild type (C57BL/6J) mice and effect of intravitreal Aflibercept on RBF in optic nerve head (ONH) region of 10 eyes of...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/180128 |
| _version_ | 1826115743390892032 |
|---|---|
| author | Su, Steven Zheng Zhe Ang, Bryan Chin Hou Balne, Praveen Kumar Tun, Sai Bo Bo Htoon, Hla Myint Schmetterer, Leopold Barathi, Veluchamy Amutha Agrawal, Rupesh |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Su, Steven Zheng Zhe Ang, Bryan Chin Hou Balne, Praveen Kumar Tun, Sai Bo Bo Htoon, Hla Myint Schmetterer, Leopold Barathi, Veluchamy Amutha Agrawal, Rupesh |
| author_sort | Su, Steven Zheng Zhe |
| collection | NTU |
| description | Purpose: To assess intra- (repeatability) and inter-observer (reproducibility) variability of laser speckle flowgraphy (LSFG) for retinal blood flow (RBF) measurement in 20 eyes of wild type (C57BL/6J) mice and effect of intravitreal Aflibercept on RBF in optic nerve head (ONH) region of 10 eyes of Ins2 (Akita) diabetic mice. Methods: ‘Mean blur rate (MBR)’ was measured for all quadrants of tissue area (MT), vessel (MV) and total area (MA) of ONH region. Changes in MT were analysed at each timepoint. Repeatability was evaluated by measuring MBR variability without changing mouse head position, and reproducibility after resetting mouse head position by another operator. Coefficient of repeatability (CR) through Bland–Altman plot method coefficient of variation (COV) and Intraclass correlation coefficient (ICC) was calculated. Intravitreal Aflibercept (1 μg) was administered to Akita eyes and intraocular pressure (IOP) was measured using a tonometer at baseline, day 7, 14, 21 and 28 post-injection. Hurvich and Tsai's criterion was used. Results: Coefficient of repeatability values of repeatability and reproducibility for all quadrants were within limits of agreement. Reliability was excellent (ICC 0.98–0.99) and reproducibility was moderate to excellent (ICC 0.64–0.96). There was a non-significant IOP increase in all Akita eyes at Day 28 (p > 0.05), and significant increase in MT in all quadrants at Day 21 and superior, inferior and temporal quadrants at Day 28 (p < 0.05). Conclusion: Laser speckle flowgraphy demonstrates excellent repeatability and moderate to excellent reproducibility in measuring RBF. Intravitreal Aflibercept injection results in a significant increase in MT up to 28 days post-injection without significant increase in IOP. |
| first_indexed | 2024-10-01T04:00:29Z |
| format | Journal Article |
| id | ntu-10356/180128 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:00:29Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1801282024-09-22T15:38:17Z Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy Su, Steven Zheng Zhe Ang, Bryan Chin Hou Balne, Praveen Kumar Tun, Sai Bo Bo Htoon, Hla Myint Schmetterer, Leopold Barathi, Veluchamy Amutha Agrawal, Rupesh Lee Kong Chian School of Medicine (LKCMedicine) School of Chemistry, Chemical Engineering and Biotechnology Tan Tock Seng Hospital Ministry of Health Holdings Singapore National Eye Centre Yong Loo Lin School of Medicine, NUS Duke-NUS Medical School SERI-NTU Advanced Ocular Engineering Medicine, Health and Life Sciences Intraocular pressure Laser speckle flowgraphy Purpose: To assess intra- (repeatability) and inter-observer (reproducibility) variability of laser speckle flowgraphy (LSFG) for retinal blood flow (RBF) measurement in 20 eyes of wild type (C57BL/6J) mice and effect of intravitreal Aflibercept on RBF in optic nerve head (ONH) region of 10 eyes of Ins2 (Akita) diabetic mice. Methods: ‘Mean blur rate (MBR)’ was measured for all quadrants of tissue area (MT), vessel (MV) and total area (MA) of ONH region. Changes in MT were analysed at each timepoint. Repeatability was evaluated by measuring MBR variability without changing mouse head position, and reproducibility after resetting mouse head position by another operator. Coefficient of repeatability (CR) through Bland–Altman plot method coefficient of variation (COV) and Intraclass correlation coefficient (ICC) was calculated. Intravitreal Aflibercept (1 μg) was administered to Akita eyes and intraocular pressure (IOP) was measured using a tonometer at baseline, day 7, 14, 21 and 28 post-injection. Hurvich and Tsai's criterion was used. Results: Coefficient of repeatability values of repeatability and reproducibility for all quadrants were within limits of agreement. Reliability was excellent (ICC 0.98–0.99) and reproducibility was moderate to excellent (ICC 0.64–0.96). There was a non-significant IOP increase in all Akita eyes at Day 28 (p > 0.05), and significant increase in MT in all quadrants at Day 21 and superior, inferior and temporal quadrants at Day 28 (p < 0.05). Conclusion: Laser speckle flowgraphy demonstrates excellent repeatability and moderate to excellent reproducibility in measuring RBF. Intravitreal Aflibercept injection results in a significant increase in MT up to 28 days post-injection without significant increase in IOP. National Medical Research Council (NMRC) Published version This study was supported by NMRC/CNIG14NOV005and NMRC/CG-INCEPTOR/Pre-Clinical CorePlatform/2017_SERI. 2024-09-18T04:48:32Z 2024-09-18T04:48:32Z 2024 Journal Article Su, S. Z. Z., Ang, B. C. H., Balne, P. K., Tun, S. B. B., Htoon, H. M., Schmetterer, L., Barathi, V. A. & Agrawal, R. (2024). Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy. Acta Ophthalmologica, 102(6), e926-e934. https://dx.doi.org/10.1111/aos.16672 1755-375X https://hdl.handle.net/10356/180128 10.1111/aos.16672 38572815 2-s2.0-85189981589 6 102 e926 e934 en CNIG14NOV005 2017_SERI Acta Ophthalmologica © 2024 The Authors. Acta Ophthalmologica published by John Wiley & Sons Ltd on behalf of Acta Ophthalmologica Scandinavica Foundation. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences Intraocular pressure Laser speckle flowgraphy Su, Steven Zheng Zhe Ang, Bryan Chin Hou Balne, Praveen Kumar Tun, Sai Bo Bo Htoon, Hla Myint Schmetterer, Leopold Barathi, Veluchamy Amutha Agrawal, Rupesh Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title | Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title_full | Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title_fullStr | Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title_full_unstemmed | Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title_short | Effect of anti-VEGF on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| title_sort | effect of anti vegf on retinal blood flow in diabetic mice using laser speckle flowgraphy |
| topic | Medicine, Health and Life Sciences Intraocular pressure Laser speckle flowgraphy |
| url | https://hdl.handle.net/10356/180128 |
| work_keys_str_mv | AT sustevenzhengzhe effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT angbryanchinhou effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT balnepraveenkumar effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT tunsaibobo effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT htoonhlamyint effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT schmettererleopold effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT barathiveluchamyamutha effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy AT agrawalrupesh effectofantivegfonretinalbloodflowindiabeticmiceusinglaserspeckleflowgraphy |