Manipulating fungal growth in engineered living materials through precise deposition of nutrients
One main challenge of emerging fungal-based engineered living materials (ELMs) lies in achieving localized multi-material properties in these structures. Although three-dimensional (3D) printing can efficiently vary local composition and properties, it has not yet been demonstrated in fungal-based E...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/180419 |
| _version_ | 1824456355611672576 |
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| author | Teoh, Jia Heng Soh, Eugene Le Ferrand, Hortense |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Teoh, Jia Heng Soh, Eugene Le Ferrand, Hortense |
| author_sort | Teoh, Jia Heng |
| collection | NTU |
| description | One main challenge of emerging fungal-based engineered living materials (ELMs) lies in achieving localized multi-material properties in these structures. Although three-dimensional (3D) printing can efficiently vary local composition and properties, it has not yet been demonstrated in fungal-based ELMs. This work thus explores the concept of using nutrients to manipulate fungal foraging behavior in 3D structures fabricated using direct ink writing (DIW) for the next generation of fungal-based ELMs. Using two fungal strains (Pleurotus ostreatus and Ganoderma lucidum), this study shows that the ink formulation used is suitable for both DIW and mycelium growth. Varying the nutrient content allows for either the inhibition or promotion of exploration and bridging of mycelium in different sections, the control of mycelium density in three dimensions and the fabrication of patterned surfaces. There is potential in fabricating patterned fungal-based ELMs and lab-on-a-chip systems to investigate the effects of other substances and microorganisms on the foraging behavior of mycelium. |
| first_indexed | 2025-02-19T03:52:47Z |
| format | Journal Article |
| id | ntu-10356/180419 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:52:47Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1804192024-10-12T16:48:52Z Manipulating fungal growth in engineered living materials through precise deposition of nutrients Teoh, Jia Heng Soh, Eugene Le Ferrand, Hortense School of Mechanical and Aerospace Engineering School of Materials Science and Engineering Singapore Centre for 3D Printing Engineering 3D printing Direct ink writting One main challenge of emerging fungal-based engineered living materials (ELMs) lies in achieving localized multi-material properties in these structures. Although three-dimensional (3D) printing can efficiently vary local composition and properties, it has not yet been demonstrated in fungal-based ELMs. This work thus explores the concept of using nutrients to manipulate fungal foraging behavior in 3D structures fabricated using direct ink writing (DIW) for the next generation of fungal-based ELMs. Using two fungal strains (Pleurotus ostreatus and Ganoderma lucidum), this study shows that the ink formulation used is suitable for both DIW and mycelium growth. Varying the nutrient content allows for either the inhibition or promotion of exploration and bridging of mycelium in different sections, the control of mycelium density in three dimensions and the fabrication of patterned surfaces. There is potential in fabricating patterned fungal-based ELMs and lab-on-a-chip systems to investigate the effects of other substances and microorganisms on the foraging behavior of mycelium. National Research Foundation (NRF) Published version The authors acknowledge funding from the National Research Foundation of Singapore and ETH Zurich, Switzerland, with the grant Future Cities Laboratory Global, Module A4: Mycelium digitalization. 2024-10-08T06:33:48Z 2024-10-08T06:33:48Z 2024 Journal Article Teoh, J. H., Soh, E. & Le Ferrand, H. (2024). Manipulating fungal growth in engineered living materials through precise deposition of nutrients. International Journal of Bioprinting. https://dx.doi.org/10.36922/ijb.3939 2424-7723 https://hdl.handle.net/10356/180419 10.36922/ijb.3939 en International Journal of Bioprinting © 2024 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Engineering 3D printing Direct ink writting Teoh, Jia Heng Soh, Eugene Le Ferrand, Hortense Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title | Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title_full | Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title_fullStr | Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title_full_unstemmed | Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title_short | Manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| title_sort | manipulating fungal growth in engineered living materials through precise deposition of nutrients |
| topic | Engineering 3D printing Direct ink writting |
| url | https://hdl.handle.net/10356/180419 |
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